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Trmt12 (NM_026642) Mouse Untagged Clone
CAT＃: MC203568
Trmt12 (untagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4632406N01Rik; AI449275; Tyw2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC055850
TGCAGGGAGAGAGCAGCCGGAGAATCTCGCTGATGGACGGCGACGGGGTACAAACACTAGCGACATGGAG AGAGAGTGTGAGGAGTCTGTAGTTGTCGCAGTTGTGACTGAGCCCCGGTTCACGCAGAGATACCGAGACT ACCTGGAGGAGCAGAAACTCTTGGACAGGCTGCACCGGGTGGCGAAGCTGCGGGATGGCGCGGTGGCTCT GCCGGTGCTGGCGGAAAGCCTTTCTGAGCAGCATCTGCAGGAGCTGAGGGATCGCGTGGCCCCAGGCAGC ACTTGTGTGCTGACGCGGCTCCCGGATCCTCTTCCTTCCAAAAAGGCCCGGGTTCGTTCACCTGCTCAGA TACTGTGTCTGGAGGTGAGGCGTTGGGTGGAGGACCGCGGAGTGACGTGGTCCGCTGAGTTGGAGGCAGA TTTGCCCCGGTCTTGGCAACGGCATGGTGACCTAATGCTGCTTAGTGAAGACTGCTTCCAAGCCACGCTG TGGAAAGGTCTGGAGCCAGAACTCTGGGAGACTGTTGCCTCGGCCCTGGGAGTTCAACGTTTGGCAAAAC GAGGGCGGGTGCTACCTGATGGGACTCGAACTCCGTCAGTGACTCTGCTGCTGGGTGACCATGGCTGGGT AGAGCATATGGATAATGGCATCCGGTATAAGTTTGATGTGACACAGTGCATGTTCTCCTTTGGAAACATC ACCGAGAAACTTCGAGTGGCGTCTCTGTCCTGTGCTGGAGAGGTGCTGGTGGATCTCTACGCAGGGATTG GTTATTTTACATTGCCCTTCCTAGTTCATGCTGGCGCTGCCTTCGTACATGCCTGTGAATGGAATCCTCA TGCTGTAGTTGCTTTAAGAAACAACTTGGAGATCAATGGAGTGGCAGACCGGTGCCAAATACATTTTGGG GACAACAGGAAACTGAAGCTCTCAGACATTGCAGATAGGGTGAACCTCGGGCTCATTCCCAGTTCTAAAG AAGGCTGGCCCGTTGCCTGCCAAGTGCTTCGGAAGGACGTTGGGGGTATTCTGCATATCCACCAAAATGT GGAGTCTTTCTCAGGAAAGACTCCACAGCCACCTGGAAGCAATAACGTGGAAAAAGAGCACTGGCCTCGT CCCCAGAAAATCACCACTGATACACAGGGAAATGGAACTACTGAGAATTTCAGGGGGGAAATATCATCGG CCAACAAACCAGAGTGGTGGAGATGGGCAGAATCTGCAGAAACTCAGATCGCCTCTCTTCTTCATCAAGT GCATGGGAAACCATGGAGGACACGAATCTTGCATGTCCACCCAGTGAAATCTTACGCCCCTCACGTGGAT CACATAGTCCTAGATCTAGAATGCCAGCCCCTGACCTCTAGTTGGCCAGAAGGAGTAGATTTACTGACAC AGTAATTGCCAGATGAGTGCCTCATCGAGGGCTGTGTTCTCACCCTTCTGTTTTCTGGTGACCTTTTAAA GTGATCTGTGGTCTGAAAATGAACTCTAGATCGATCCAAAGTTGTCTTCTGTTGATCACATTGAGAATGT ATTAAAGTATCAGAGGAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_026642 |
| Insert Size | 1341 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055850, AAH55850 |
| RefSeq Size | 1581 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 68260 |
| UniProt ID | Q8BG71 |
| Gene Summary | S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203250 | Trmt12 (Myc-DDK-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12) |
CNY 3,656.00 |
|
| MR203250L3 | Lenti ORF clone of Trmt12 (Myc-DDK-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12) |
CNY 5,230.00 |
|
| MR203250L4 | Lenti ORF clone of Trmt12 (mGFP-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (Trmt12) |
CNY 5,230.00 |
