Gtf2b (NM_145546) Mouse Untagged Clone

CAT#: MC203695

Gtf2b (untagged) - Mouse general transcription factor IIB (Gtf2b), (10ug)



  "NM_145546" in other vectors (4)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms MGC6859
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC016637
CGGAGCTGTTGGCGGAGCCGCGAAGATGGCGTCGACCAGCCGTTTGGATGCTCTCCCAAGAGTCACATGT CCGAATCATCCAGATGCAATTTTAGTTGAAGACTACAGAGCTGGCGATATGATCTGTCCCGAATGTGGCC TAGTTGTAGGGGACCGAGTTATTGATGTGGGATCTGAATGGAGAACTTTCAGCAATGACAAAGCAACAAA AGACCCATCTAGAGTTGGAGACTCTCAGAATCCTCTTCTGAGTGATGGAGATTTGTCCACCATGATTGGC AAGGGTACAGGAGCTGCAAGTTTTGATGAGTTTGGCAATTCTAAGTATCAGAACCGGAGAACAATGAGTA GCTCTGATCGAGCAATGATGAACGCATTCAAGGAAATCACGACCATGGCGGACAGAATCAACCTCCCTCG CAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTGTATGAGCAGAAGAGCCTGAAGGGCAGAGCT AATGACGCGATAGCCTCTGCTTGTCTGTACATCGCCTGTAGACAAGAAGGCGTTCCCAGGACATTTAAAG AAATATGTGCTGTGTCTCGAATCTCTAAGAAAGAAATTGGCCGCTGTTTTAAACTGATTTTGAAAGCTCT GGAAACCAGCGTGGATCTGATCACAACTGGGGACTTCATGTCCAGGTTCTGCTCCAACCTTTGCCTCCCC AAGCAAGTGCAGATGGCAGCTACACACATAGCCCGCAAGGCAGTGGAGCTGGACTTGGTTCCTGGCAGGA GCCCGATCTCTGTGGCGGCAGCAGCTATTTACATGGCTTCCCAGGCTTCAGCTGAGAAGCGAACACAGAA AGAAATCGGGGATATTGCTGGTGTTGCTGATGTTACAATCCGACAGTCCTACAGACTGATCTACCCTCGG GCTCCGGATCTCTTCCCTTCAGACTTCAAGTTTGACACCCCAGTGGACAAATTACCCCAGCTATAAGCTG AGGCAACTAAGTGTCACACTCTTAATGTACATGGAATATGGAACTTTGTACATAGCCACACAGTGCTGGT TGAGCCTTTAATGAGGAAAAAGGAGATGGTACCCATTCCAGAGCTAAATATTATTGCTTAGTCTTCTGTA TGTATATACTAGTGCAACATATTTAATGACTTAAATTTCTTACTGAATCTGCTTTCTTTTTTAGTGATCT AGGAAACAGTATTTTGGAAGGTATTAAAATAATGTAATTCTCAAATAAAAATTTAAAACCAAAAAAAAAA AAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_145546
Insert Size 951 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC016637, AAH16637
RefSeq Size 1267 bp
RefSeq ORF 951 bp
Locus ID 229906
UniProt ID P62915
Gene Summary General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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