Dis3 (NM_028315) Mouse Untagged Clone

CAT#: MC204173

Dis3 (untagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (Dis3), (10ug)



  "NM_028315" in other vectors (4)

CNY 6,328.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2810028N01Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC027357
GCGGGAATGCGCGTGGCCGCGCCTCGGTTTCTTAGCTGACGGCCGGGATCCCACGCCGCGAGGCGAGATG CTCAGGTCCAAGACGTTCTTGAAGAAGACCCGCGCGGGCGGCGTGGTGAAGATCGTGCGCGAGCACTACC TGCGGGATGACATCGGCTGCGGCGCGCCGGCTTGCTCGGCCTGCGGCGGGGCGCACGCGGGCCCGGCCCT GGAGCTGCAGCCCCGGGACCAGGCGAGCAGCCTCTGCCCGTGGCCGCACTACCTTCTGCCGGACACCAAT GTGCTGCTGCACCAGATTGATGTCCTCGAACACCCGGCCATCAAAAATGTCATTGTGCTACAAACAGTGA TGCAAGAAGTGAGAAACCGGAGCGCCCCCATCTACAAGCGAATCAGGGATGTGACCAATAACCAGGAAAA GCATTTCTATACCTTCACTAATGAGCACCATAAAGAAACCTACATCGAGCAAGAGCAGGGAGAGAATGCC AATGACAGGAATGACAGAGCCATCCGAGTCGCAGCGAAGTGGTACAACGAGCACCTGAAGAGGGTGGCAG CAGACAGTCAGCTGCAAGTTATCCTGATAACCAATGACAGGAAGAACAAAGAGAAAGCTGTGCAAGAGGG GATACCAGCCTTCACGTGTGAAGAATACGTAAAGAGCCTGACTGCTAACCCTGAACTTATAGACCGTCTT GCTTACTTGTCCGATGAAATGAATGAAATAGAAAGTGGGAAAATAATATTTTCAGAGCATCTTCCCTTAA GCAAGCTCCAACAAGGCATAAAATCTGGTTCCTATCTTCAAGGAACATTCAGAGCTAGCAGGGAAAATTA TTTGGAGGCTACAGTATGGATTCATGGAGACAAAGAAGAGGAAAAAGAGATACTTATACAGGGAATTAAG CATCTAAACAGAGCTGTGCATGAAGACATTGTGGCCGTGGAGCTACTGCCCAGGAGCCAGTGGGTGGCAC CGTCTTCCGTGGTTTTAGACGATGAAGGTCAAAATGAAGACGATGTGGAGAAAGATGAGGAGAGAGAACT CCTGCTTAAGACTGCTGTAAGTGAAAAAATGTTACGGCCTACAGGTCGAGTTGTGGGGATAATAAAAAGG AACTGGAGACCGTATTGTGGCATGCTTTCCAAGTCTGATATTAAGGAGTCAAGAAGACATCTCTTTACAC CCGCTGATAAGAGAATTCCACGAATTCGGATAGAAACCAGACAGGCTTCTGCGTTAGAAGGACGGAGAAT TATTGTCGCTATTGATGGTTGGCCTAGAAATTCTAGATATCCAAATGGACACTTTGTAAAGAATTTAGGC GATGTTGGAGAGAAGGAGACAGAAACGGAAGTGTTGCTGCTCGAGCACGATGTTCCTCATCAGCCCTTTT CCCAGGCTGTGCTTAGCTTCCTGCCCAGGATGCCCTGGAGCATTACTGAGGAGGACATGAAAAACCGAGA AGACCTGAGACATCTGTGTGTTTGCAGTGTGGACCCTCCAGGGTGCACTGACATAGATGACGCTCTGCAT TGTAGAGAGCTCAGCAATGGAAACTTGGAGGTTGGTGTTCATATTGCGGATGTTAGCCATTTTATCAGGC CAGGAAATGCGTTGGATCAAGAATCTGCAAGAAGAGGAACAACTGTTTATCTTTGTGAAAAGAGGATTGA CATGGTTCCAGAGTTGCTCAGCTCCAACCTCTGTTCCTTAAGATCCAACGTTGACAGGCTTCTCTTACGT ACGCGGAAGCACAGATGAGAATTGATTCGGCGGCTATGAATGATGATATTACCACTAGTCTCCGTGGACT CAATCAGCTGGCTAAAATTCTAAAAAAGGGAAGGATTGAAAAGGGGGCTTTGACTCTGTCTTCTCCAGAG ATCCGATTCCACATGGACAGTGAAACCCACGACCCAATCGACCTGCAGACGAAAGAGCTGAGAGAAACAA ATTCCATGGTGGAAGAATTTATGTTACTTGCTAATATTTCTGTCGCAAAAAAAATTCATGAAGAGTTTTC TGAACATGCTCTGCTTCGGAAACACCCAGCTCCGCCTCCCTCGAATTATGACATTCTTGTTAAGGCTGCA AAGTCCAAGAATTTGCAAATTAAGACTGATACAGCCAAATCTTTGGCCGACTCTTTGGACCGGGCTGAAT CTCCGGATTTCCCATACCTGAATACTCTATTAAGGATACTGGCCACTCGCTGTATGATGCAAGCTGTGTA CTTCTGCTCTGGGATGGATAATGATTTTCATCACTATGGCTTAGCCTCCCCCATATACACACATTTCACC TCTCCTATCAGAAGATATGCAGACATAATTGTCCATCGGCTATTAGCTGTGGCGATTGGGGCTGACTGTA CTTACCCTGAGTTGACAGACAAACACAAGCTTTCAGATATATGTAAAAACCTCAATTTCCGGCATAAAAT GGCTCAGTATGCCCAGCGTGCTTCGGTGGCTTTTCATACACAGTTGTTTTTCAAAAGCAAAGGAATAGTA AGTGAAGAAGCCTATATTCTCTTTGTAAGGAAAAATGCAATTGTGGTGTTAATTCCAAAGTATGGCTTAG AAGGTACAGTTTTCTTTGAAGAAAAAGATAAACCAAAGCCACGCCTTGCTTACGATGATGAGATCCCTTC CCTGAGAATAGAAGGTACAGTGTTCCATGTGTTTGATAAGGTTAAAGTGAAGATCACATTAGATTCATCA AATCTTCAACATCAGAAAATCCGCATGGCCCTTGTAGAACCACAGATCCCAGGAATAAATATTCCTCCTA ATGTTGCAGACAAGGCTCTTACTGCACCAGGGGGAAAGAAGAGGAAGCTTGAGAAGTAGCTACGTTCAAC AGAACATCCAAAGACTCAACAGCAACAAAATGGAGAGCATACTTACAGACTCCTGTGCGATAGCTACTTT TAAATACATTTCAATAATTTTAACAAATTTGAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_028315
Insert Size 1701 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC027357, AAH27357
RefSeq Size 2996 bp
RefSeq ORF 1701 bp
Locus ID 72662
UniProt ID Q9CSH3
Gene Summary Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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