S1pr5 (NM_053190) Mouse Untagged Clone

CAT#: MC204585

S1pr5 (untagged) - Mouse sphingosine-1-phosphate receptor 5 (S1pr5), (10ug)



  "NM_053190" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Edg8; lpB4; S1P5
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC012232
CGATAACCTTGAAGTCCTGATCCTCCGCTGGCAATTGCTGGGGAACCGGTGTACACCAAATGCCCAGCTT ACTCCCCCCAGATTTCCAATAGCCGCTCTCCTGTGTCTGCTTCTGGAGTGCCGGTTACAGGAGACTTTTG CACAGTGCTCCAGTGGACGACCGGCAGGACACAGCAGCGACATCTCCGCGGCGGGCGGCGGGCAGCCCAT GGAGCCCGGGCTGCTGCGGCCGGCGCCGGTGAGTGAGGTTATTGTCCTTCACTACAACTACACCGGCAAG CTCCGCGGGGCGCGCTACCAGCCCGGCGCCGGCCTGCGCGCAGACGCCGCAGTGTGTCTGGCTGTGTGTG CCTTCATTGTGCTGGAGAACTTGGCTGTGCTCTTGGTGCTGGTCCGCCACCCTCGCTTCCATGCGCCCAT GTTTCTGCTCCTTGGCAGCCTCACTTTGTCGGACCTGCTCGCCGGGGCGGCCTACGCCACCAACATCCTA CTGTCCGGGCCGCTCACGCTGCGCCTGTCGCCTGCGCTTTGGTTTGCGCGTGAGGGGGGCGTCTTCGTGG CGCTCGCCGCGTCGGTGCTGAGCCTCTTGGCCATTGCTTTAGAGCGCCACCTTACCATGGCCCGTCGTGG ACCCGCACCCGCCGCCAGTCGCGCTCGCACGCTGGCAATGGCGGTGGCCGCCTGGGGCGCGTCGCTATTG CTCGGGCTGCTACCCGCGCTGGGCTGGAACTGCTTAGGACGCCTGGAAACCTGCTCCACCGTGCTGCCGC TCTACGCCAAGGCCTATGTGCTCTTCTGCGTGCTGGCCTTCCTGGGCATCCTGGCTGCCATCTGTGCGCT CTATGCAAGGATTTACTGTCAGGTGCGGGCCAACGCGCGTCGCCTGCGGGCGGGTCCTGGGTCCCGCAGG GCCACCTCGTCCTCGCGATCCCGGCACACGCCGCGGTCGCTGGCCCTGCTTCGTACCCTTAGCGTGGTGC TCCTGGCCTTCGTGGCCTGCTGGGGACCGCTGTTTCTCTTGCTATTACTGGATGTCGCGTGCCCAGCCCG CGCGTGTCCCGTGCTCCTGCAGGCCGATCCCTTCCTGGGTCTAGCCATGGCTAACTCGCTGCTGAATCCC ATCATCTACACCTTCACCAACCGAGATCTGCGCCACGCGCTCCTGCGGCTGCTCTGCTGTGGCCGCGGAC CCTGCAACCAAGACTCCTCCAACAGCTTGCAGCGATCCCCCAGTGCTGCCGGGCCTTCCGGTGGTGGCCT GCGACGCTGCCTGCCACCAACCTTGGATCGCAGCTCTAGCCCATCAGAACACTTGTCTCCCCAGCAGGAC GGGGTGGACACCAGCTGCTCCACCGGCAGTCCTGGAGTAGCAACCGCCAACCGGAGCCTGGTGCCTACTG CTACAGACTGACGCTTCCCAACCCTTTGCAAGAACAGACTGACTCGCCAAATAAGCGCATCATCTGTAGG AAATAGCCAGCGACGGAGGGGGACATACAAGAAAGAAGGGAAAGTGATTGATCACCCCATAACAAGAACA GACAAAAATGCACTCGGGATCTGAGATGGACAATAAGGGAAAGAAGAAAGTGATGAGAAGGTATCCAGGT AGTGGAACCTAAGCTGGGAAGCCCTGAAGGAGAGCCATTCCATATGGCTGCAGCAGAAATTGAGGAGCAG GAGATGAAAGGGCAGGTTGTGGGGAAGTCTTGTTGGTTCTGAGGAAGTCCTTAGGTTTGGCTCTGAGTGA AGTGGGAGCCATAGAGAATTCTAAACAAAGGAGGGACTCTCCTGGATTCAGACCTCCACAGGTACCCTGT GACTACCAAAGTTCAAGGGGAATGGCTAAAAGATGAAGGGATAAAGGAGAGCGGGGCTCCAAGAATGCTC AGAACCAGGTGATGTCTAGATAAGAGTGTGCTAAGGGCTGGCAGGAGTAGTGGCTGGCTACAGAGGAAAC AGAGCTGTGGACAAAGGTAAGCCTAGGTCTGGAAATTTGGCTCTGGAGACTGTCAGATATCAGCTGCCAA ACTTCCAGAGTGTGGACAGGTTCTTCATACAAATTACAACCACAATAAAAACAATTCAAGGAGTTGTCCT TACTGGGATCTTTCAGAATCTATTCATTCATCTATTGATTTTTTTTTTAATTTTCCCCCAGAAAGTTGTA ACTATCCTCTTTAAAAGTGTTTATTTATTGGGGTTGGAGAGATGGCTCAGCAGTTAAGAACACTTGTTAC CCTTGCAGGGGACCTGTGTTCCATTCCCAGCACCCAAGTGGTGATTCTGGCAACTTCAGTTCCAAGGTTA TCTGATGCCCTCATCTGACCTCTGAGGGCACCAGGCATGCACATGGTACAAAGATTGAATGCAGGCAAAA CTCTCATACAAACAAGCTAAATAAATCTTTTAAAATGTTAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_053190
Insert Size 1203 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC012232, AAH12232
RefSeq Size 2437 bp
RefSeq ORF 1203 bp
Locus ID 94226
UniProt ID Q91X56
Gene Summary Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). S1P activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival. S1P activation on O4-positive pre-oligodendrocytes induces process retraction via a Rho kinase/collapsin response-mediated protein signaling pathway. The S1P-induced survival of mature oligodendrocytes is mediated through a pertussis toxin-sensitive, Akt-dependent pathway. S1P activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival. These effects depend on the developmental stage of the cell.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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