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Was (NM_009515) Mouse Untagged Clone
CAT＃: MC217446
Was (untagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was), (10ug)
CNY 3,880.00
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | U42471; Wasp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217446 representing NM_009515
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAGTGGCCCTGGCCCTGTAGGAGGCAGGCCTGGGGGCCGAGGGGGACCAGCCGTTCAGCAGAACA TTCCTTCCAACCTCCTCCAGGACCATGAAAACCAGAGACTCTTTGAGCTTCTTGGCCGAAAATGCTGGAC ACTGGCTACCACAGTTGTTCAGCTCTACCTGGCACTGCCCCCTGGAGCTGAGCACTGGACCATGGAACAC TGCGGGGCTGTGTGCTTCGTGAAGGATAACCCTCAGAAGTCCTACTTCATCCGCCTTTATGGCCTACAGG CTGGTCGGCTACTCTGGGAACAGGAGCTGTACTCTCAGCTGGTTTATCTCACTCCCACCCCGTTCTTCCA CACTTTTGCTGGAGATGACTGTCAAGTAGGACTGAACTTTGCGGATGAGAGTGAAGCCCAGGCCTTCCGG GCCTTGGTGCAGGAGAAGATACAAAAAAGGAATCAGAGGCAAAGCGGAGAAAGACGCCAGCTACCACCAC CACCAGCACCAATCAATGAGGAGAGAAGAGGAGGGCTCCCACCTGTGCCCCCACACCCGGGTGGAGATCA TGGGGGCCCATCAGGTGGTCCACTATCTCTAGGACTTGTGACGGTCGACATTCAGAACCCTGACATCACA AGTTCACGATACCGTGGGCTCCCTGCACCTGGCCCTGGCCCAACTGATAAGAAACGCTCAGGGAAAAAGA AGATCAGCAAAGCTGATATCGGAGCACCGAGTGGATTCAAACATGTCAGCCACGTGGGCTGGGATCCCCA GAATGGATTTGATGTGAACAACCTAGACCCGGATCTGCGGAGCTTGTTCTCCAGGGCAGGAATCAGCGAG GCCCAGCTCACTGACGCAGAGACCTCCAAGCTCATCTACGATTTTATTGAGGACCAGGGAGGTCTAGAGG CTGTCCGGCAGGAGATGAGGCGCCAAGAGCCACTCCCACCACCTCCGCCGCCATGCAGAGGAGGAGGAGG AGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCCAGCCTCTGAGACCTCCTGTTGTGGGG AGTAATAAGGGTCGCTCAGGTCCACTGCCCCCTGTACCTATGGGGGGTGCCCCACCTCCACCAACACCAC GAGGGCCCCCACCACCAGGCCGAGGGGGTCCTCCTCCACCACCCCCTCCAGCCACTGGACGATCTGGACC ACCACCTCCTCCACTCCCTGGAGCTGGGGGACCACCAGCACCGCCACCACCACCACCACCACCACCACCT CCACCCTGCCCTGGGAGTGGGCCCGCCCCTCCCCCGCTCCCTCCTACTCCAGTGTCTGGGGGGAGCCCAG CACCTGGTGGGGGCCGGGGTGCACTTTTGGACCAAATCCGGCAGGGAATTCAGCTGAACAAGACCCCTGG AGCTCTAGAGAACTCAGTACAGCAACCACCCGCGCAGCAGTCAGAAGGCCTAGTAGGTGCCCTGATGCAT GTCATGCAGAAGAGGAGTAGAGTCATCCATTCCTCAGATGAAGGGGAGGATCAGACCGGCGAGGATGAAG AGGATGATGAATGGGATGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009515 |
| Insert Size | 1563 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009515.2, NP_033541.1 |
| RefSeq Size | 2094 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 22376 |
| UniProt ID | P70315 |
| Gene Summary | Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226777 | Was (tGFP-tagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was), (10ug) |
CNY 5,040.00 |
|
| MR226777 | Was (Myc-DDK-tagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was) |
CNY 3,872.00 |
|
| MR226777L3 | Lenti ORF clone of Was (Myc-DDK-tagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was) |
CNY 6,460.00 |
|
| MR226777L4 | Lenti ORF clone of Was (mGFP-tagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was) |
CNY 6,460.00 |
