Snx27 (NM_029721) Mouse Untagged Clone

CAT#: MC217483

Snx27 (untagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2, (10ug)



  "NM_029721" in other vectors (4)

CNY 6,750.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
SNX27 Antibody - middle region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5730552M22Rik; ESTM45; ESTM47; R75405
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217483 representing NM_029721
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGAGGACGGGGAAGGTATTCACCCCTCAGCCCCGCACCGGAATGGAGGCGGCGGCGGGGGGT
CTGGGCTCCACTGCGCCGGGAACGGTGGCGGGGGAGGCGGCGGCCCGCGGGTCGTGCGCATTGTCAAGTC
CGAATCCGGCTACGGCTTCAACGTGCGGGGCCAAGTGAGCGAGGGCGGGCAACTGCGGAGCATCAACGGG
GAGCTGTACGCGCCGCTGCAGCATGTGAGCGCCGTGCTGCCCGGGGGGGCGGCCGACCGGGCCGGGGTGC
GCAAAGGGGACCGCATCCTGGAGGTGAACGGCGTGAATGTTGAGGGGGCGACACACAAGCAGGTGGTGGA
CCTGATCCGAGCAGGCGAGAAGGAATTGATCTTGACAGTGTTGTCTGTACCTCCTCATGAGGCTGATAAC
CTAGATCCCAGTGACGACTCCTTGGGACAATCATTTTATGATTACACAGAAAAGCAAGCAGTGCCCATAT
CGGTCCCCACATACAAACATGTGGAGCAGAATGGTGAGAAGTTTGTGGTATACAATGTTTACATGGCTGG
GAGGCAGCTGTGTTCTAAGCGGTACCGGGAGTTTGCCATACTGCACCAAAACCTGAAGAGAGAATTTGCC
AACTTTACATTTCCTCGACTTCCAGGAAAGTGGCCATTCTCATTATCAGAACAACAATTGGATGCCCGGC
GTCGTGGGTTGGAAGAATATCTAGAAAAGGTGTGTTCAATCCGAGTCATTGGTGAGAGTGACATCATGCA
GGAATTCCTGTCTGAGTCTGATGAGAATTACAATGGTGTGTCTGATGTAGAACTGAGAGTAGCACTACCG
GATGGAACAACGGTTACTGTCAGAGTTAAGAAGAACAGTACTACAGACCAAGTGTACCAGGCTATTGCAG
CAAAGGTTGGCATGGACAGTACAACAGTGAATTACTTTGCCTTATTTGAAGTGATCAATCATTCCTTTGT
ACGTAAGCTGGCACCTAATGAATTTCCTCATAAACTCTACGTCCAGAACTATACATCAGCTGTGCCAGGC
ACTTGCCTGACCATTCGCAAGTGGCTCTTTACAACAGAAGAAGAAGTCCTCTTAAATGACAATGACCTTG
CTGTTACGTACTTCTTTCATCAGGCTGTTGATGATGTAAAGAAAGGTTACATTAAAGCAGAAGAGAAATC
TTACCAGTTACAGAAGCTCCATGAACAAAGGAAGATGGTCATGTACCTCAACATGCTAAGGACCTGCGAG
GGCTACAATGAAATCATCTTCCCTCACTGTGCCTGTGACTCGAGGAGGAAGGGACATGTTATCACAGCCA
TCAGCATCACGCACTTTAAACTTCATGCATGCACTGAAGAAGGGCAGCTGGAGAACCAGGTAATAGCGTT
TGAATGGGATGAGATGCAGCGATGGGACACAGATGAAGAAGGAATGGCCTTCTGTTTTGAATATGCGCGA
GGAGAGAAGAAGCCTCGATGGGTTAAAATCTTCACACCATATTTCAATTACATGCATGAATGCTTTGAGA
GGGTGTTCTGCGAGCTCAAGTGGAGAAAAGAGGAATATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_029721
Insert Size 1581 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_029721.2, NP_083997.1
RefSeq Size 6273 bp
RefSeq ORF 1581 bp
Locus ID 76742
UniProt ID Q3UHD6
Gene Summary Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate 3' terminal exon which results in a frameshift compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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