Pard3 (NM_001122850) Mouse Untagged Clone

CAT#: MC221245

Pard3 (untagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 4, (10ug)



  "NM_001122850" in other vectors (4)

CNY 9,600.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA960621; AI256638; Asip; D8Ertd580e; Par-3; Par3; Pard-3; Pard3a; Phip
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221245 representing NM_001122850
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGTGACCGTGTGCTTCGGGAGGACCCGGGTGGTCGTGCCGTGCGGAGATGGCCGCATGAAAGTTT
TCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCGTGGCCAAGGATCCAAACTACTGGATACA
GGTGCATCGCTTGGAGCATGGAGATGGAGGGATTCTAGACCTGGATGACATCCTCTGTGACGTTGCTGAT
GACAAAGACAGACTGGTAGCAGTATTTGATGAACAGGATCCCCACCATGGAGGAGATGGTACCAGCGCCA
GCTCCACGGGAACCCAGAGTCCAGAGATATTCGGCAGTGAGCTGGGCACCAACAATGTTTCTGCTTTTCA
GCCTTATCAAGCCACAAGTGAAATTGAGGTCACGCCTTCAGTTCTTCGGGCAAATATGCCTCTTCATGTC
CGCCGGAGCAGCGACCCAGCTTTAACTGGCCTTTCCACTTCTGTCAGTGATAACAACTTTTCCTCAGAGG
AGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCAGAACACCGCTGGAAG
TCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTTCCACGGGATCCCAGTAGCTGGTCC
AACCAGTTCCAGCGAGACAACGCCCGCTCCTCCCTGAGCGCCAGCCACCCAATGGTAGACCGGTGGCTGG
AGAAGCAAGAACAGGATGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCCGGTTGGACATGCTGA
TACCGGATTGGAGAACATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTACAAGTCCCCAACGAT
GGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACATTGGGGTTGTTAGTGA
AGCGGTTGGAGAAAGGCGGTAAGGCTGAGCAAGAAAACCTTTTCCATGAGAATGACTGCATTGTGAGGAT
TAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAACATATGTTCCGCCAAGCTATGCGTGCG
CGTGTCATTTGGTTCCATGTGGTCCCTGCAGCAAACAAGGAGCAATATGAACAACTGTCCCAACGCGAGA
AGAACAACTACTCCCCAGGCCGCTTCAGCCCTGACAGCCACTGTGTGGCCAACAGGAGTGTGGCCAACAA
TGCCCCTCAAGCATTGCCCAGAGCACCCAGACTGAGTCAGCCACCCGAGCAGCTGGATGCTCACCCCCGA
CTACCTCATAGTGCTCACGCCTCAACCAAACCACCCGCAGCCCCGGCCTTGGCTCCACCCAGTGTGCTTA
GTACCAACGTAGGCAGTGTGTACAACACGAAGAAAGTAGGCAAGAGGCTCAACATCCAGCTTAAGAAAGG
TACAGAAGGACTGGGATTCAGCATCACCTCCCGGGACGTCACCATAGGTGGCTCAGCTCCCATTTATGTC
AAGAATATCCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGACTCAAGGCAGGAGACCGGCTAATAGAGG
TCAATGGAGTAGATTTAGCAGGCAAATCCCAGGAGGAAGTTGTTTCCCTGTTGAGAAGCACCAAGATGGA
GGGGACTGTGAGCCTTCTGGTCTTTCGTCAGGAAGAGGCTTTCCACCCAAGGGAAATGAATGCTGAACCA
AGCCAGATGCAGACTCCAAAAGAAACGAAAGCTGAAGATGAGGACGTTGTTCTCACACCCGATGGTACCA
GGGAGTTTCTGACTTTTGAAGTTCCACTGAATGACTCAGGATCTGCAGGGCTTGGTGTCAGTGTCAAGGG
GAACCGTTCCAAAGAGAACCACGCAGATTTGGGGATCTTCGTTAAATCCATTATCAATGGTGGAGCTGCA
TCTAAAGATGGAAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAATGGAGAATCTCTACTGGGCAAAG
CCAACCAGGAAGCCATGGAGACTCTACGGAGGTCCATGTCCACCGAGGGCAACAAGCGTGGCATGATCCA
GCTCATTGTGGCGAGGCGGATCAGCAGATGTAACGAGCTGCGGTCTCCTGGGAGCCCTGCTGCACCTGAG
CTGCCCATCGAGACAGAACTGGATGACCGAGAACGCAGGATCTCACACTCCCTCTACAGTGGGATCGAGG
GGCTGGATGAGTCGCCCACCAGGAACGCAGCACTGAGCAGGATAATGGGAACATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001122850
Insert Size 2226 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001122850.1, NP_001116322.1
RefSeq Size 4178 bp
RefSeq ORF 2226 bp
Locus ID 93742
Gene Summary Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (4) differs in the 3' UTR and coding region compared to variant 5. The resulting isoform (4) has a shorter and distinct C-terminus compared to isoform 5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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