Arhgap44 (NM_175003) Mouse Untagged Clone

CAT#: MC221458

Arhgap44 (untagged) - Mouse Rho GTPase activating protein 44 (Arhgap44), transcript variant 2, (10ug)



  "NM_175003" in other vectors (4)

CNY 9,880.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6330543G20; AI840762; AU040829; AW493732; Rich2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221458 representing NM_175003
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAAGCAGTTTAACCGCATGCGCCAGTTGGCCAACCAGACGGTGGGCAGGGCAGAAAAGACTGAAG
TTCTGAGCGAAGACCTTCTCCAGGTGGAGAAGCGCCTAGAGCTGGTGAAGCAGGTGTCCCACAGCACACA
CAAGAAGCTCACCGCATGTCTGCAGGGTCAGCAGGGAGCAGAGGCCGACAAGCGATCAAAAAAGTTGCCT
CTGACGACTCTGGCGCAGTGCTTGGTGGAGGGGTCCGCGATCCTGGGCGATGACACGCTTCTTGGGAAGA
TGCTGAAGCTCTGCGGAGAGACGGAGGACAAGCTAGCTCAGGAGCTGATCCACTTTGAGCTGCAAGTGGA
GAGGGATGTGATCGAGCCCCTGTTCCTGCTCGCTGAGGTGGAAATTCCAAATATTCAAAAGCAGAGGAAA
CATTTAGCCAAGCTGGTGCTTGACATGGACTCTTCCCGGACCAGGTGGCAGCAGACTTCCAAGTCTTCAG
GGCTTTCCAGCAGCCTTCAGCCTGCGGGTGCCAAAGCCGATGCCCTCAGGGAGGAGATGGAGGAGGCTGC
CAACAGAGTGGAGATTTGCCGGGACCAGCTTTCTGCTGACATGTACAGTTTTGTGGCCAAAGAAATTGAC
TATGCAAACTACTTTCAAACGCTAATAGAAGTGCAAGCTGAGTACCACAGGAAGTCGCTGACACTGCTGC
AGGCTGTGTTGCCTCAGATCAAAGCCCAGCAGGAGGCCTGGGTGGAGAAGCCTTCCTTTGGGAAGCCCCT
GGAGGAGCACCTGATGATCAGTGGCCGAGAGATCGCCTTCCCTATCGAGGCGTGTGTGACCATGCTGCTG
GAGTGTGGGATGCAGGAGGAGGGCCTCTTCCGGGTAGCCCCCTCAGCCTCCAAGCTGAAGAAGCTGAAAG
CTGCTCTGGACTGCTGTGTGGTGGATGTCCAGGAGTACTCGGCTGACCCCCACGCCATAGCAGGGGCTTT
GAAGTCTTATCTCCGAGAGTTGCCAGAGCCTCTTATGACCTTTGAACTCTATGACGAATGGATCCAGGCT
TCTAATATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCA
ACCATAACAACATCAAATACTTGATCAAATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGAT
GACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACC
GAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGT
TCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGC
CAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGG
CCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGGCATGGGTGTGAGGGTCA
TGGACACATCTTGGGTGGCACGAAGAGGCTCCTCTGCTGGTCGGAAAGCATCCTGCGCCCCGCCTTCCAT
GCAGCCTCCTGCCCCACCCTCCGAGCTGGCTGCACCCTTGCCTTCACCCCTGCCGGAGCAGGTCCCAGAC
AGCCCTGCCACTCCAGCTCCTGCGCTCTCTCCATCAGGTGCCAGCCTGCAGCCCACCCCCGAGCGCCCCA
GTGTGTCTAAAAGCAAGGAACTGTCGCCAGGGTCTGGGCAGAAAGGAAGTCCAGGTTCCATCCAGGGGAC
ACCCTGCCCAGGGACGCAACTGGGGCCACAGCCGGCTGCCAGCCCCAGCCAGCTTCCTGCAGACCAGAGT
CCTCACACCCTCCGGAAAGTTTCAAAGAAGGTGGCACCAATTCCTCCCAAGGTCCCCTTCGTGCAGCCAG
GGACTGTTTCAGATCAGCCCGTTGGCCAGCCATCCCCAGTCAGCCTGTCTCCTACTCCACCAAGCACCCC
ATCCCCCTATGGACTGAGCTACCCTCCAGGCTACTCCATGGCCTCTGGCCAGCTTTCTCCTGCCTCAGCT
CCTCCCCTGGCCTCTCCCTCTGTCTTTACAAGCACTTTAGCCAAATCTCGGCCCACCCCTAAGCCTCGAC
AGAGGCCCACCCTGCCGCCTCCTCAGCCTCCCAGTGTCAGCCTGTCGGCTTCAAGTCCACAGTCCACAGA
GCACCCCATGTTAGACGGCATGTCGCCGGGGGAAAGCATGTCTACAGCTGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175003
Insert Size 2295 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175003.3, NP_778168.2
RefSeq Size 4099 bp
RefSeq ORF 2295 bp
Locus ID 216831
UniProt ID Q5SSM3
Gene Summary GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1 (PubMed:24352656, PubMed:26969129). In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (PubMed:23739967, PubMed:24352656, PubMed:26969129). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (PubMed:23739967).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) contains an alternate exon and differs in the 3' UTR, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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