Rasgrp1 (NM_011246) Mouse Untagged Clone

CAT#: MC221698

Rasgrp1 (untagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1), (10ug)



  "NM_011246" in other vectors (4)

CNY 10,260.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Rasgrp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221698 representing NM_011246
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTGCCATGGCTCCAGAGCTGGCCCCAAAG
CAAGACTAGAGGCCAAATCAACCAACAGCCCTCTCCCTGCCCAGCCCAGCTTGGCCCAGATCACCCAGTT
CCGAATGATGGTGTCCCTGGGACATTTGGCCAAAGGAGCCAGCCTGGATGATCTTATTGACAGCTGCATT
CAATCTTTTGATGCGGATGGAAACCTGTGTCGGAATAACCAACTGTTACAAGTCATGCTAACCATGCACC
GGATCATCATCTCCTCAGCCGAGCTGCTACAAAAAGTCATGAATCTATATAAGGATGCCCTGGAAAAGAA
TTCTCCAGGAGTTTGCCTGAAGATCTGCTATTTTGTCAGGTATTGGATAACAGAATTCTGGATCATGTTC
AAGATGGATGCCAGCTTGACCAGCACCATGGAAGAGTTCCAAGACCTGGTGAAAGCCAATGGTGAGGAGA
CCCACTGCCACCTCATCGACACGACCCAAATTAATTCTCGAGACTGGTCCAGGAAACTGACTCAGAGGAT
AAAATCGAATACTAGCAAGAAGCGCAAAGTGTCCCTGCTGTTTGACCATCTGGAACCTGAAGAACTGTCT
GAACACCTCACCTACCTTGAGTTCAAGTCCTTCCGACGGATATCTTTCTCTGATTACCAAAATTACCTTG
TAAATAGCTGCGTAAAAGAGAACCCCACCATGGAGAGGTCCATTGCCCTGTGCAATGGCATCTCCCAGTG
GGTACAACTGATGGTTCTCAGCCGTCCCACCCCGCAGCTCCGGGCAGAGGTCTTCATCAAGTTCATCCAT
GTGGCTCAGAAACTCCACCAGCTACAGAACTTCAACACGCTGATGGCTGTGATCGGGGGACTGTGTCACA
GCTCCATCTCCAGGCTTAAGGAAACAAGTTCACATGTCCCACATGAGATCAATAAGGTTCTGGGCGAGAT
GACTGAACTGCTGTCCTCCTGCAGAAACTATGACAACTACAGGCGAGCCTATGGGGAGTGCACCCACTTC
AAAATCCCCATACTGGGTGTGCACCTCAAGGACCTCATATCCCTGTATGAGGCTATGCCCGACTATCTGG
AAGATGGGAAGGTGAATGTCCAAAAGCTCCTGGCCCTTTACAATCATATCAATGAATTGGTCCAGCTGCA
GGAGATGGCCCCACCATTGGATGCCAACAAGGACTTGGTGCATCTGCTGACGTTATCCCTGGATCTATAC
TATACAGAAGATGAAATCTATGAGCTTTCCTATGCCCGGGAGCCGAGGAACCACAGAGCTCCGCCACTGA
CACCTTCGAAGCCACCAGTTGTAGTAGACTGGGCCTCTGGAGTGTCTCCCAAACCTGACCCAAAGACCAT
CAGCAAACACGTCCAAAGGATGGTGGATTCTGTCTTTAAGAACTATGATCTCGACCAGGATGGCTATATC
TCTCAGGAGGAGTTTGAGAAGATCGCTGCAAGCTTTCCATTTTCCTTCTGTGTGATGGACAAAGATAGGG
AAGGCCTCATCAGCAGAGACGAGATCACGGCCTACTTCATGAGGGCCAGCTCCATCTATTCCAAGCTGGG
CCTGGGCTTTCCACACAACTTTCAAGAAACCACTTACCTGAAGCCCACCTTCTGTGACAACTGTGCTGGC
TTTCTCTGGGGCGTGATCAAGCAAGGCTATCGCTGTAAAGACTGCGGGATGAACTGCCACAAACAGTGCA
AAGACCTGGTGGTGTTCGAGTGCAAGAAGCGAATCAAGAGCCCAGCAATATCCACAGAGAACATCAGCTC
TGTGGTACCAATGTCCACCCTTTGTCCACTGGGAACCAAAGATCTGCTCCATGCACCCGAAGAAGGATCT
TTCATTTTCCAAAACGGAGAGATTGTGGACCACAGTGAGGAGAGCAAGGATAGGACCATCATGCTCCTCG
GAGTGTCCTCCCAGAAAATTTCAGTTCGGCTGAAGAGGACTGTTGCCCACAAGAGCACCCAAACAGAATC
GTTCCCGTGGGTTGGTGGCGAGACGACCCCTGGTCACTTTGTGCTGTCTTCTCCAAGGAAGTCGGCGCAG
GGCGCTCTTTATGTGCACAGTCCAGCATCTCCATGCCCCAGCCCAGCACTGGTCCGAAAGCGGGCATTCG
TCAAGTGGGAGAACAAAGAGTCCCTTATAAAACCAAAACCAGAACTTCACCTCCGGCTCCGGACCTACCA
AGAACTGGAACAGGAAATAAATACCCTGAAAGCAGATAACGATGCTCTGAAGATCCAACTGAAGTACGCA
CAGAAGAAAATAGAATCCCTGCAGCTTGGGAAAAGCAATCATGTCTTAGCCCAGATGGACCACGGTGATA
GTGCTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_011246
Insert Size 2388 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011246.2, NP_035376.1
RefSeq Size 5155 bp
RefSeq ORF 2388 bp
Locus ID 19419
UniProt ID Q9Z1S3
Gene Summary Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (By similarity). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses (PubMed:17190838). May also function in differentiation of other cell types. Proto-oncogene, which promotes T-cell lymphomagenesis when its expression is deregulated (PubMed:15829980, PubMed:17210708).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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