Agap1 (NM_001037136) Mouse Untagged Clone

CAT#: MC221789

Agap1 (untagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 2, (10ug)



  "NM_001037136" in other vectors (4)

CNY 10,360.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Centg2; Ggap1; mKIAA1099; R75364
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221789 representing NM_001037136
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTCGAGTCGGTCC
ACCCCAACATCTACTCCATCTACGAGCTGCTGGAGCGCGTGGAGGAGCCGGTGCTGCAGAACCAGATTCG
GGAGCACGTCATCGCCATCGAAGATGCTTTCGTGAACAGCCAGGAATGGACACTCAGCCGATCGGTACCG
GAGCTCAAAGTGGGAATTGTGGGTAACCTGGCCAGTGGCAAGTCTGCGCTGGTGCACCGGTACCTGACAG
GCACGTACGTGCAGGAGGAATCTCCAGAAGGTGGCAGGTTCAAGAAGGAGATTGTTGTTGATGGACAGAG
TTATCTGCTGCTGATTAGGGATGAAGGGGGCCCCCCGGAGGCACAGTTCGCCATGTGGGTGGACGCGGTC
ATCTTTGTCTTCAGCTTGGAGGATGAGATCAGTTTCCAGACTGTCTACCATTACTACAGCCGAATGGCCA
ACTACAGGAACACCAGTGAGATCCCATTAGTGCTGGTGGGGACCCAGGATGCCATAAGTTCCACCAACCC
GAGGGTCATCGATGACGTCAGGGCTCGGAAGCTCTCCAATGACCTGAAGAGGTGCACGTACTATGAGACG
TGCGCCACCTATGGGCTCAACGTGGAGCGGGTCTTCCAGGACGTTGCCCAGAAGATCGTTGCCACAAGGA
AGAAGCAGCAGCTATCCATAGGCCCCTGCAAGTCCTTACCCAACTCTCCAAGCCACTCGTCTGTGTGTTC
TGCCCAAGTGTCCGCGGTACACATCAGCCAGACAAGTAACGGAGGTGGGAGTTTAAGTGACTACTCATCC
TCTGTTCCTTCGACCCCAAGCACCAGCCAGAAAGAGCTTCGGATCGACGTCCCTCCCACTGCCAACACTC
CAACGCCTGTCCGGAAGCAGTCCAAGCGCAGGTCCAACCTATTCACATCTAGGAAAGGGAGCGACCCAGA
CAAAGAGAAGAAAGGCCTGGAGAGCCGTGCGGACAGCATCGGGAGCGGGCGAGCCATCCCAATTAAACAG
GGCATGCTGCTGAAGCGAAGTGGCAAGTCACTGAACAAAGAGTGGAAAAAGAAGTACGTCACCCTGTGCG
ACAACGGTGTGCTGACCTACCACCCCAGTTTACATGATTACATGCAGAATGTCCACGGCAAAGAGATAGA
CCTCTTGAGGACCACTGTGAAGGTGCCGGGGAAGAGGCCGCCCCGAGCCACGTCTGCCTGTGCACCGATC
TCCAGTCCCAAAACCAACGGCCTGGCCAAGGACATGAGCAGTTTACACATCTCACCAAATTCAGACACGG
GGCTGGGTGACTCGGTGTGTTCCAGCCCAAGTATCTCCAGCAGCACCAGCCCCAAGCTCGACCCACCCCC
CTCCCCCCACGCCAACAGAAAGAAGCACCGGAGGAAGAAAAGTACCAGCAACTTCAAGGCTGACGGGCTC
TCAGGCACTGCTGAAGAGCAAGAAGAAAACTTGGAGTTTATCATTGTGTCCCTCACTGGCCAGACGTGGC
ACTTTGAAGCCACCACATACGAGGAGCGAGACGCATGGGTCCAAGCCATCGAGAGCCAGATCCTCGCCAG
CCTGCAGTCGTGCGAGAGCAGCAAGAATAAGTCCCGACTAACCAGCCAGAGCGAAGCCATGGCCCTGCAG
TCCATCCGTAACATGAGAGGGAACTCGCACTGCGTGGACTGTGACACCCAAAACCCTAACTGGGCCAGTT
TGAACTTGGGAGCCCTCATGTGTATCGAGTGCTCGGGGATCCACCGGAATCTCGGCACCCACCTCTCTCG
GGTCAGATCGCTAGACCTGGACGACTGGCCCATGGAGCTGATCAAGGTGATGTCATCCATCGGGAATGAG
CTGGCCAACAGCGTGTGGGAAGAGGGCAGCCAGGGACGGACAAAGCCCTCACTGGACTCCACCAGGGAAG
AAAAGGAAAGGTGGATCCGGGCCAAGTATGAGCAGAAACTCTTCCTGGCCCCACTGCCATGCACAGAGTT
CTCCCTGGGCCAGCAGCTGCTGCGTGCCACTGCCGAGGAAGACCTGCGGACTGTCATCCTACTTCTGGCA
CATGGGTCCCGGGACGAGGTGAATGAGACCTGTGGGGAAGGAGACGGTCGCACGGCGCTGCACCTGGCCT
GTCGCAAGGGCAACGTGGTCCTGGCTCAGCTGCTGATCTGGTACGGAGTGGACGTCATGGCCCGAGACGC
CCACGGGAACACAGCACTGGCCTATGCCCGGCAGGCCTCCAGCCAGGAGTGTATTGACGTGCTTCTACAG
TATGGCTGCCCCGACGAGCGCTTTGTGCTCATGGCTACCCCTAACCTGTCCAGGAAGAGCAACAGCAGGA
ACAACAGTAGCGGGAGGGCGCCCAGTGTCATCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001037136
Insert Size 2415 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001037136.1, NP_001032213.1
RefSeq Size 9422 bp
RefSeq ORF 2415 bp
Locus ID 347722
UniProt ID Q8BXK8
Gene Summary GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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