Nedd4 (NM_010890) Mouse Untagged Clone

CAT#: MC222415

Nedd4 (untagged) - Mouse neural precursor cell expressed, developmentally down-regulated 4 (Nedd4), (10ug)



  "NM_010890" in other vectors (6)

CNY 9,752.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA959633; AL023035; AU019897; E430025J12Rik; mKIAA0093; Nedd4-1; Nedd4a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222415 representing NM_010890
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCGGACATGGCAGCCGACGAGTCGGAGGCCCCAGTACTCTCGGAGGACGAGGTATGGGAGTTTT
GCCTGGATAAGACAGAAGATGGTGGCGGATCCCCCGGAAGTGATGTTACAGATACTTGTGAGCCTCCATG
TGGATGCTGGGAGTTGAATCCGAATTCCCTGGAAGAGGAGCACGTGCTGTTCACTGCTGATCCGTACCTG
GAGCTCCACAACGATGACACACGAGTTGTGAGAGTGAAGGTTATAGCTGGCATAGGCCTGGCCAAGAAAG
ACATCTTGGGAGCCAGTGATCCTTACGTAAGAGTGACATTGTATGACCCGATGAGTGGAATCCTTACCAG
CGTGCAGACAAAAACTATCAAAAAGTCTTTGAATCCAAAATGGAATGAAGAAATACTGTTCAGGGTCCTT
CCACAGCGACACCGCATTCTTTTCGAAGTGTTTGATGAAAATCGTTTGACAAGAGATGATTTCCTAGGTC
AAGTGGATGTCCCTCTCTATCCTTTACCGACTGAAAACCCAAGAATGGAGAGACCATATACATTTAAGGA
TTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGATTAAAAATGACTTATTTA
CCTAAAAATGGCTCAGAAGATGAAAATGCAGACCAGGCTGAGGAGTTAGAGCCTGGCTGGGTTGTTTTGG
ACCAACCAGATGCTGCCACTCATTTGCCGCATCCACCAGAACCCTCTCCCCTACCTCCAGGATGGGAAGA
GAGGCAGGATGTCCTTGGAAGGACCTACTACGTAAACCATGAATCTAGAAGAACACAGTGGAAAAGGCCA
AGCCCTGACGATGACCTCACGGATGAAGACAATGATGATATGCAGCTGCAAGCGCAGCGAGCATTCACCA
CCAGGCGGCAGATATCGGAGGATGTGGATGGCCCTGACAACCGGGAGTCCCCTGAGAATTGGGAAATCGT
ACGAGAAGATGAAAACACCGAGTATAGTGGTCAGGCTGTCCAGTCACCTCCATCGGGTCACATTGATGTG
CAGACTCACCTTGCAGAAGAGTTTAATACCAGACTTGCTGTGTGTGGAAATCCAGCCACCAGCCAGCCGG
TTACCAGCTCAAATCATTCCAGCAGAGGAGGCAGCTTGCAGACCTGTATCTTTGAGGAACAGCCTACACT
TCCTGTGCTTTTGCCTACTTCATCTGGATTGCCACCAGGTTGGGAAGAAAAACAAGATGACAGAGGAAGA
TCATACTATGTAGACCACAACTCTAAAACCACCACATGGTCCAAGCCCACCATGCAGGATGATCCAAGAT
CGAAAATCCCTGCTCATCTGAGAGGAAAGACTGACTCCAATGACCTGGGACCCTTACCTCCAGGCTGGGA
AGAAAGAACCCACACAGATGGGCGAGTCTTCTTCATAAACCACAATATAAAGAAGACCCAGTGGGAAGAT
CCTCGCCTGCAGAACGTGGCAATCACTGGACCAGCAGTGCCCTACTCCAGAGATTACAAGAGAAAGTACG
AGTTCTTCAGAAGGAAGCTCAAGAAGCAGACTGACATTCCAAACAAATTTGAAATGAAGCTTCGCCGCGC
AAACATTCTGGAGGATTCTTACCGGAGGATTATGGGTGTGAAGAGAGCTGACTTGCTCAAGGCCAGACTC
TGGATTGAGTTTGATGGTGAAAAGGGCCTTGACTATGGAGGGGTTGCCAGAGAGTGGTTCTTCCTCATCT
CGAAGGAAATGTTCAACCCTTACTACGGCCTGTTTGAATATTCTGCTACGGATAATTACACCCTACAGAT
AAATCCTAACTCGGGCTTGTGTAATGAAGATCACCTCTCATACTTCAAGTTCATTGGCCGTGTGGCTGGG
ATGGCAGTTTATCATGGCAAGCTGTTGGATGGGTTTTTCATCCGTCCGTTTTACAAGATGATGCTTCAGA
AACTGATAACACTGCACGACATGGAGTCCGTGGATAGTGAATATTACAGTTCTCTGCGATGGATTCTTGA
AAATGACCCGACGGAGCTGGACCTGAGATTTATCATAGATGAAGAACTTTTTGGACAGACACATCAGCAC
GAACTGAAAACCGGAGGATCAGAGATTGTTGTCACCAATAAGAACAAAAAGGAGTATATCTACCTTGTAA
TACAATGGCGATTTGTGAACCGTATCCAGAAGCAAATGGCAGCTTTTAAAGAGGGATTTTTTGAACTGAT
ACCACAGGATCTCATCAAGATATTTGATGAAAATGAGCTAGAGCTTCTCATGTGTGGTCTGGGAGATGTG
GATGTGAACGACTGGCGGGAACACACAAAATACAAAAATGGCTACAGCATGAACCACCAGGTCATCCACT
GGTTCTGGAAGGCTGTTTGGATGATGGATTCGGAAAAAAGAATACGCTTACTTCAGTTTGTCACTGGCAC
ATCCCGTGTGCCGATGAATGGGTTTGCTGAACTCTATGGCTCGAATGGACCACAATCCTTCACAGTGGAA
CAATGGGGCACCCCTGATAAGCTGCCAAGAGCACACACCTGCTTCAATCGCCTGGACCTGCCACCCTACG
AATCCTTTGACGAACTCTGGGATAAACTTCAGATGGCAATTGAGAACACACAGGGCTTTGATGGCGTTGA
TTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_010890
Insert Size 2664 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010890.3, NP_035020.2
RefSeq Size 5494 bp
RefSeq ORF 2664 bp
Locus ID 17999
UniProt ID P46935
Gene Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (By similarity). Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Involved in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19193720). Predominantly involved in ubiquitination of membrane bound forms of ERBB4 rather than processed precursors and intermediate membrane-anchored 80 kDa fragments (m80HER4), with a lesser role in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19047365). Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (By similarity). Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...