Dgki (NM_001081206) Mouse Untagged Clone

CAT#: MC223463

Dgki (untagged) - Mouse diacylglycerol kinase, iota (Dgki), (10ug)



  "NM_001081206" in other vectors (4)

CNY 13,780.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms C130010K08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223463 representing NM_001081206
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCCG
CCGCCTCCAGCGCCCTCAGCCCGACCGGCCTCTGCAGCGGCACCACCTCGGCTTCTTTCGCGGCCGCCGG
AGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAGAGGGGCGACGGGTGGCAGCAGCAGCAGC
GGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGCGGACCCGCGGGGTGCAGGGGCAGCGG
CTGCTGCTGCCTTGGAGGAGCCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCT
GAGGGACTTAACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG
GCACCTGCACATCCTCTTGGTCTTCCTGTGGCTAACGGTCCAGCGAAGGAGCCCAGAGCCACTCTGGACT
GGAGCGAGAATGCTGTGAATGGGGAGCACCTGTGGCTGGAGACCAACGTTTCAGGGGACCTCTGCTACCT
GGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCTCTCAGGAGGAAGTGTGCCGTCTGCAAAATC
GTGGTCCACACAGCCTGCATTGAGCAACTAGAGAAGATTAATTTCAGATGTAAACCAACATTTCGGGAAG
GAGGCTCCAGATCGCCAAGAGAAAACTTTGTGCGTCACCACTGGGTCCACAGGAGGCGGCAGGAGGGAAA
ATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC
TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCATATTGAGGAACCCT
GCTCCCTGGGGGCTCACGCCGCTGTTATTGTCCCGCCCACCTGGATCATTAAGGTGAAGAAACCTCAGAA
CTCCCTGAAGGCTTCCAACCGGAAGAAGAAGAGGACAAGTTTTAAAAGGAAAGCCAGTAAAAGAGGAACT
GAGCAGGAAACCAAAGGCCGTCCTTTTGTGATCAAGCCCATCTCCTCCCCTCTCATGAAGCCTTTGCTTG
TCTTTGTGAACCCAAAGAGTGGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAA
TCCACGGCAAGTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGATGTACAGGAAGGTACCA
AATCTGAGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTGGACGAGCTGC
AGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCACTGGGGACTGGGAATGACCTGGCTCGGACGCTCAA
CTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATCCTGTGCCAGGTGGAAGATGGGACCATTGTA
CAGTTGGATCGCTGGAACCTCCACGTAGAGAGAAACCCAGACCTGCCGCCCGAAGAACTTGAAGATGGCG
TGTGTAAGCTTCCTCTGAATGTCTTCAATAATTACTTCAGCCTTGGATTTGATGCTCACGTCACATTGGA
GTTCCATGAATCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCA
GGGGCAGCCTTTTCTGATTTCCTACAAAGAAGTTCTAGAGATCTATCCAAACACGTCAAAGTTGTCTGTG
ATGGGACAGACCTCACCCCAAAGATCCAGGACCTGAAGTTCCAGTGTATAGTATTTTTAAATATTCCCAG
ATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGATCATCATGACTTTGAACCCCAACGTCATGAT
GACGGCTACATTGAAGTCATTGGATTCACTATGGCCTCTTTGGCTGCCCTGCAAGTTGGGGGGCACGGAG
AAAGGCTGCACCAGTGTCGGGAAGTGATGCTTCTGACTTACAAGTCCATTCCCATGCAAGTGGACGGGGA
GCCATGTCGGTTGGCACCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC
AAGCGGAGAACCTCGATGCCTTTGCTCAATGACATCCATCAGGTGCAAGCTGCGGACCTGCGGCGAGTGT
CTGCTCCCCCCGGCTCCTTCACCATTCCCCAGTCTGTCCCAGATCGCCTGAGGATCCGTGTGAACAAAAT
CAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGACAAACTCCGGGAAGCTTCCATCCCTCTGGGT
ATTCTAGTTGTTCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGTCTACAGGAGGACT
TGCAGTCAGTTTCTTCTGGGTCACAGAGAGTTCATTACCAGGACCAAGAAACCTCCTTCCCCAGGGCACT
CTCAGCTCAGAGGCTGTCCCCACGGTGGTGCTTCCTAGATGCAACGTCTGCTGACCGCTTTTATCGAATA
GACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCACGATGAAATTTTTATTCTGGACCCAG
ATATGGTGGTGTCACAGCAGGCAGGGACCCCTCCAGGAATGCCTGACCTGGTGGTTGAACAGGCTTCTGG
GCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATGCTGAGTGACAGTGGGATGATCACACCCCAC
TATGAGGACTCAGATCTGAAAGACTTCAGCCACTCCAGAGTGCTACAGTCCCCAGTCTCTTCAGAAGATC
ATGCAATTTTGCAGGCAGTCCTCACCGGTGACCTTATGAAGCTCATGGAGAGCTATAAGAATGGAGGGAG
CTTACTAATTCAGGGACCAGGGCACTGTTCTCTCCTTCACTACGCAGCTAAGACCGGCAATGGGGACATT
GTGAAGTACATCCTTGACCACGGACCTGCAGAATTACTGGATATGGCAGACAGTGAAACGGGCGAGACTG
CACTGCACAAGGCTGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTGGTGGATGCAGGAGCATCTCT
GAGACAGACAGACTCCAAGGGCAAGACACCCCAGGAACGAGCACAGCAGGCCGGGGACCCAGATTTGGCT
GCTTACCTAGAAAGCCGACAGAACTATAAGATCATTGGCCATGAAGACCTGGAAACTGCTGTTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001081206
Insert Size 3216 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081206.1, NP_001074675.1
RefSeq Size 4598 bp
RefSeq ORF 3216 bp
Locus ID 320127
UniProt ID D3YWQ0
Gene Summary Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position (By similarity). By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway (PubMed:15894621). May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression (PubMed:21119615).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...