Tet3 (NM_183138) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B430006D22Rik; BC037432; D230004J03Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224802 representing NM_183138 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCAGGGCCAGTGTACCATGGAGATTCAAGGCAGCTAAGCACCTCAGGGGCGCCGGTCAATGGTG CTAGAGAGCCCGCCGGACCCGGTCTTCTGGGAGCTGCGGGTCCTTGGCGGGTAGACCAGAAGCCCGACTG GGAGGCTGCCTCAGGCCCCACTCACGCTGCTCGTCTGGAAGATGCCCACGACCTGGTGGCCTTTTCGGCC GTGGCCGAAGCTGTGTCATCTTACGGGGCCCTTAGTACCCGGCTCTATGAAACCTTCAACCGTGAGATGA GTCGTGAGGCTGGGAGCAACGGCAGGGGCCCCCGGCCTGAGAGCTGCTCTGAGGGCAGTGAAGACCTGGA CACGCTGCAGACAGCCCTGGCCCTTGCAAGGCATGGCATGAAACCACCCAACTGCACCTGCGATGGCCCA GAGTGCCCCGACTTCCTCGAGTGGCTGGAGGGCAAGATCAAGTCTATGGCCATGGAGGGAGGGCAGGGGC GGCCTAGGCTCCCAGGCGCTCTGCCTCCCAGTGAGGCTGGCCTCCCAGCCCCTAGCACCAGACCGCCACT CCTTAGCTCTGAGGTCCCCCAGGTACCTCCCCTGGAGGGCCTGCCTCTGTCCCAGAGCGCGCTGAGCATT GCCAAGGAAAAAAACATCAGCCTGCAGACAGCCATCGCCATCGAGGCCCTCACACAGCTCTCCTCCGCCC TCCCTCAGCCTTCTCATTCCACCTCCCAGGCTTCTTGTCCACTCCCTGAGGCCTTGTCCCCTTCTGCCCC TTTCAGGTCTCCCCAGTCCTACCTCCGGGCCCCCTCATGGCCTGTGGTTCCCCCAGAGGAACATCCATCC TTTGCTCCTGACAGCCCAGCCTTCCCTCCAGCAACCCCAAGACCTGAGTTTTCTGAAGCGTGGGGCACTG ACACCCCCCCAGCGACACCCCGGAACTCCTGGCCTGTACCTCGCCCAAGCCCTGACCCTATGGCAGAACT GGAGCAGCTATTGGGCAGCGCCAGTGATTACATCCAGTCAGTATTCAAGCGGCCTGAGGCCCTGCCCACC AAGCCCAAGGTCAAGGTTGAGGCCCCCTCTTCTTCCCCTGCTCCGGTACCATCTCCTATTTCTCAGAGGG AGGCTCCCCTGCTGTCTTCAGAGCCTGACACCCACCAGAAGGCCCAGACAGCCCTTCAGCAACATCTTCA TCACAAGCGCAACCTATTCTTGGAACAGGCCCAAGATGCCTCCTTCCCTACTTCCACAGAGCCTCAGGCT CCTGGTTGGTGGGCCCCTCCCGGCTCACCTGCCCCAAGGCCTCCTGACAAACCACCCAAGGAAAAGAAAA AGAAGCCCCCCACCCCTGCTGGAGGTCCCGTGGGAGCAGAGAAAACCACCCCTGGGATCAAGACCAGTGT CCGAAAGCCCATTCAGATCAAGAAATCCAGGTCCAGGGACATGCAGCCCCTCTTCCTGCCTGTTAGGCAG ATTGTTCTGGAAGGGCTAAAACCCCAAGCCTCAGAAGGACAGGCACCGTTACCCGCCCAGCTCTCTGTCC CACCTCCTGCCTCCCAGGGTGCTGCATCCCAGAGCTGTGCCACCCCTCTAACCCCAGAACCTTCTCTTGC GCTATTTGCACCTAGTCCCTCCGGGGACAGCCTGCTGCCCCCTACTCAGGAAATGAGATCCCCCAGCCCC ATGGTAGCCCTGCAGTCAGGCTCCACTGGTGGCCCCCTTCCCCCTGCCGATGACAAGCTGGAGGAGCTCA TCCGGCAATTTGAGGCTGAATTTGGGGATAGCTTTGGGCTTCCCGGCCCACCTTCGGTGCCCATTCAAGA ACCTGAAAACCAATCAACATGTCTCCCAGCTCCGGAGAGCCCTTTTGCCACCCGCTCCCCCAAGAAGATC AAGATCGAGTCCTCAGGGGCCGTGACTGTGCTCTCAACTACCTGCTTCCATTCAGAAGAGGGGGGACAGG AGGCCACGCCCACCAAGGCTGAGAACCCACTCACACCAACCCTCAGTGGCTTCTTGGAGTCACCTCTAAA GTACCTAGACACACCTACTAAGAGTCTGCTGGACACACCGGCCAAGAAGGCTCAGTCCGAGTTCCCTACC TGCGATTGTGTCGAACAAATAGTGGAGAAAGATGAAGGCCCATATTACACTCACCTGGGATCTGGCCCCA CAGTAGCTTCTATCCGGGAACTCATGGAGGATCGGTATGGAGAAAAGGGGAAAGCTATCCGGATTGAGAA GGTCATCTACACGGGCAAGGAGGGGAAGAGTTCTCGAGGCTGTCCCATCGCCAAGTGGGTGATCCGAAGA CACACACTGGAGGAGAAGCTGCTGTGCCTGGTGCGGCATCGGGCAGGCCACCATTGTCAGAACGCCGTGA TTGTTATCTTGATCCTGGCCTGGGAGGGCATCCCTCGAAGCCTTGGGGACACCCTCTACCAGGAGCTTAC TGATACCCTCCGGAAGTATGGCAACCCTACCAGCCGGAGATGTGGCCTCAATGATGACCGGACCTGTGCT TGCCAAGGCAAAGACCCTAACACCTGCGGTGCCTCCTTCTCCTTCGGCTGTTCCTGGAGCATGTACTTCA ACGGCTGCAAATATGCTCGGAGCAAGACGCCACGAAAGTTCCGCCTCACGGGAGACAATCCGAAGGAGGA GGAGGTGCTCCGGAATAGCTTTCAGGATCTGGCCACTGAAGTTGCTCCCCTCTACAAGCGGCTCGCACCC CAGGCCTATCAGAACCAGGTGACCAATGAGGATGTGGCGATCGACTGCCGCCTGGGGCTGAAGGAAGGGA GACCCTTCTCAGGGGTCACAGCCTGCATGGACTTCTGTGCCCACGCCCACAAGGACCAACATAACCTCTA CAATGGGTGCACTGTGGTCTGCACCCTGACCAAGGAAGACAATCGCTGCGTGGGCCAGATCCCTGAGGAC GAGCAACTGCACGTGCTGCCCCTCTACAAGATGGCCAGCACGGATGAGTTTGGCAGCGAGGAAAACCAGA ACGCCAAGGTCAGTAGTGGGGCCATCCAGGTGCTCACAGCATTCCCCAGAGAGGTCCGGCGGCTGCCTGA GCCTGCCAAGTCCTGCCGCCAACGGCAGCTGGAAGCCAGGAAGGCGGCGGCCGAGAAGAAGAAGCTGCAG AAGGAGAAACTGAGCACGCCAGAGAAGATCAAGCAGGAGGCCCTGGAGTTGGCTGGAGTCACCACTGACC CAGGCCTGTCTCTGAAGGGTGGATTGTCCCAGCAAAGCCTGAAGCCCTCCCTCAAGGTGGAGCCTCAGAA CCACTTTAGCTCCTTTAAGTACAGTGGCAATGCGGTGGTGGAAAGCTACTCGGTGCTGGGCAGCTGCCGG CCCTCCGACCCCTACAGCATGAGCAGTGTGTATTCCTACCATTCGCGCTATGCACAGCCTGGCCTGGCCT CTGTCAACGGCTTCCACTCCAAGTACACACTTCCCTCCTTTGGCTACTATGGCTTTCCATCAAGCAACCC TGTCTTCCCCTCCCAGTTCCTGGGTCCCAGTGCCTGGGGGCATGGGGGCAGTGGAGGCAGTTTTGAGAAG AAGCCAGACCTCCATGCTCTACACAACAGCCTGAACCCAGCCTACGGTGGTGCTGAGTTTGCCGAGCTGC CAGGTCAGGCTGTTGCCACAGACAACCACCACCCCATCCCTCACCACCAGCAGCCTGCTTACCCAGGCCC CAAGGAATATCTGCTACCCAAGGTCCCCCAGCTCCACCCAGCATCCAGGGACCCCTCTCCCTTTGCTCAG AGTTCCAGTTGCTACAACAGATCCATCAAGCAAGAGCCAATAGACCCTCTGACCCAGGCTGAGTCCATTC CCAGAGACTCTGCTAAGATGAGTAGAACACCCTTGCCGGAAGCATCTCAGAATGGGGGACCCAGTCATCT GTGGGGACAGTACTCAGGAGGCCCAAGCATGTCCCCGAAGAGGACTAACAGTGTAGGTGGCAACTGGGGC GTGTTCCCTCCGGGGGAGAGCCCTACCATTGTTCCCGACAAGCTCAATTCTTTTGGGGCCAGCTGTCTCA CTCCTTCACACTTCCCAGAAAGCCAGTGGGGACTGTTCACTGGTGAAGGCCAGCAGTCGGCCCCCCATGC TGGAGCACGGCTTCGAGGCAAGCCATGGAGCCCCTGCAAGTTTGGGAACGGCACCTCTGCCTTGACTGGT CCCAGCCTAACTGAGAAGCCATGGGGGATGGGAACCGGGGATTTCAACCCCGCCCTGAAAGGTGGACCTG GGTTCCAAGACAAGTTGTGGAATCCTGTGAAGGTGGAGGAGGGCAGGATTCCCACACCGGGGGCCAACCC GCTAGACAAAGCCTGGCAAGCCTTTGGCATGCCCTTGAGCTCCAACGAGAAGCTATTTGGGGCCCTGAAG TCAGAGGAGAAACTGTGGGATCCCTTCAGCCTGGAGGAGGGGACAGCTGAGGAGCCCCCCAGCAAGGGGG TGGTGAAGGAAGAGAAGAGTGGACCCACAGTGGAAGAGGACGAGGAGGAACTGTGGTCGGACAGTGAACA CAACTTCCTGGATGAGAACATAGGCGGGGTGGCCGTGGCCCCCGCCCATTGCTCCATCCTCATCGAGTGT GCCCGGCGAGAGCTGCATGCCACCACTCCACTCAAAAAACCCAACCGCTGCCACCCCACCCGCATCTCGC TGGTCTTCTACCAACACAAGAACCTCAACCAGCCCAACCACGGGCTGGCGCTCTGGGAGGCCAAGATGAA GCAGCTGGCGGAACGGGCGCGGCAGCGGCAAGAGGAGGCCGCACGCCTGGGCCTGGGCCAGCAGGAGGCC AAGCTCTACGGGAAGAAGCGAAAATGGGGGGGTGCTATGGTGGCTGAGCCCCAGCACAAAGAAAAGAAGG GGGCTATCCCTACCCGGCAGGCGCTGGCCATGCCCACAGACTCCGCGGTCACCGTGTCCTCTTACGCCTA CACAAAGGTCACTGGCCCCTACAGCCGCTGGATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_183138
Insert Size	5007 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_183138.2, NP_898961.2
RefSeq Size	10907 bp
RefSeq ORF	5007 bp
Locus ID	194388
Gene Summary	Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks multiple exons in the coding region, and uses an in-frame downstream start codon, compared to variant 1. Variant 2 encodes a protein (isoform 2) with a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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