Plxnd1 (NM_026376) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6230425C21Rik; b2b553Clo; b2b1863Clo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225047 representing NM_026376 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGTCGCGCCGCGGGCGGCGCACCCCCTAGCGCCCGGGCCGCCGCGGCCGTCCCCTTGCGTCCGC GCCCTCACTCGCGGGGCCCTGGTCTGCTGCCGCTGCCTCTGCTGCTGCTGCTCGGGGCGGCACGGGCCGG CGCCCTAGAGATCCAGCGCCGTTTCCCCTCGCCCACGCCCACCAACAACTTCGCCCTGGACGGCACGGCG GGCACCGTGTACTTGGCGGCAGTGAACCGCCTGTACCAACTGTCGAGTGCCAACTTGAGCCTGGAAGCCG AGGCGACCGTGGGTCCCGTGCCGGACAGCCCGCTGTGTCACGCCCCGCAGCTCCCGCAGGCCTCGTGCGA GCACCCGCGGCGCCTCACGGACAACTACAACAAAATCCTGCAGTTGGACCCGGGCCAGGGTCTGGTGGTC GCGTGCGGCTCCATCTACCAGGGTCTGTGCCAGCTGAGGCGCCGGGGCAACATCTCAGCCCTGGCCGTGA GCTTTCCGCCTGCCGCGCCGACCGCAGAACCGGTCACCGTGTTCCCCAGCATGCTCAACGTGGCCGCCAA CCACCCCAACGCGTCCACTGTGGGACTGGTGCTGCCGCCTACCTCGGGCACCGGGGGCAGCCGTCTGCTC GTGGGCGCCACGTACACCGGCTTCGGCAGCGCTTTCTTCCCGCGCAACCGTAGCCTAGAAGACCACCGCT TCGAGAACACGCCCGAGATCGCTATCCGCTCCCTGGACGCGCGTGGAGACTTGGCCAAGCTCTTCACCTT CGACCTTAACCCGTCGGACGATAACATCCTGAAGATCAAGCAGGGCGCCAAGGAGCAGCACAAGCTGGGC TTCGTGCGTGCCTTCTTGCACCCGGCGGTGCCACCGCACAGCGCGCAGCCCTACGCGTACCTGGCGCTCA ACAGCGAGGCGCGTGCGGGCGACAAGGACAGCCAGGCGCGCAGCCTGCTGGCGCGCATCTGCCTGCCCCG CGGCGCGGGTGGCGACGCCAAGAAGCTCACCGAGTCCTACATCCAACTGGGCTTGCAGTGCGCGGGCGGC GCGGGCCGCGGCGACCTCTACAGCCGCCTCGTGTCGGTTTTCCCCGCGCGCGAGCAGTTCTTCGCTGTCT TCGAGAGGCCCCAGGGCGCCCCAGGTGCCCGCAACGCCCCGGCCGCGCTTTGCGCCTTCCGCTTCGACGA CGTGCAGGCTGCCATTCGTGCAGCGCGCACCGCCTGCTTCGTGGAGCCGGCGCCCGACGTGGTGGCGGTG TTGGACAGTGTGGTGCAGGGCACCGGGCCGGCCTGCGAGAGCAAGCGCAACATACAGCTGCAGCCGGAGC AACTGGATTGCGGAGCGGCCCACCTGCAGCACCCACTGACCATCCTGCAGCCGCTGAGGGCATCGCCCGT GTTCCGTGCTCCAGGGCTCACGGCCGTGGCTGTGGCCAGTGCCAACAACTACACGGCCGTCTTTCTGGGC ACCGCCACAGGGAGGCTCCTCAAGATCAGCCTGAACGAGAGCATGCAGGTAGTAAGCAGGCGAGTGCTGA CTGTAGCCTATGGGGAGCCTGTGCATCACGTCATGCAGTTTGACCCCATGGATCCTGGTTACCTATACCT GATGACATCCCACCAGATGGCCCGAGTGAAGGTGGCAGCGTGTGAGGTACACTCCACCTGCGGGGACTGC GTGGGTGCGGCCGATGCCTACTGTGGTTGGTGCACTCTGGAGACCCGGTGCACACTCCAGCAGGATTGCA CCAACTCCAGCCAGCCACATTTCTGGACCAGTGCCAGTGAGGGCCCCAGCCGCTGCCCTGCCATGACAGT ACTGCCCTCGGAGATTGATGTGCACCGGGACTACACAGGTATGATCTTACAGATCTCAGGAAGCCTGCCC AGCCTCAGCGGCATGGAGATGGCTTGTGACTATGGAAATGGCGTTCGAACGGTGGCCCGGGTACCTGGCC CTGCCTATGATCATCAGATTGCCTACTGCAATCTCCTGCCCAGGGCCCAGTTTCCATCCTTTCCTGCTGG CCAGGACCACGTGACCGTTGAGATGTCTGTAAGGGTCAAAGGACACAACATTGTCTCAGCCAATTTCACC ATCTACGACTGCAGCCGAATTGGACAAGTCTACCCCCATACAGCCTGTACCAGCTGCCTGTCCACACAGT GGCCTTGCTCCTGGTGCATCCAGCTGCATTCATGTGTCTCCAACCAGTCTCAGTGCCAGGACTCGCCAAA CCCCACGAGTCCTCAGGACTGTCCCCAGATCCTGCCCTCGCCCCTAGCGCCCGTGCCCACAGGTGGCTCC CAAGACATCCTGGTGCCCCTGACTAAAGCCACCTTCTTCCATGGTTCCTCCCTCGAGTGCAGCTTTGGGC TGGAAGAGAGCTTTGAGGCTGTATGGGCGAATAACTCACTGGTCCGCTGCAACCAAGTGGTGCTGCACAC AACCCAGAAGAGCCAGGTATTTCCACTGAGTCTGAAGCTGAAGGGGCCGCCAGACCGATTCCTAGACAGC CCTAACCCCATGACAGTTGTGGTCTACAACTGTGCTATGGGCAGCCCTGACTGTTCCCAGTGCCTGGGCC GTGAGGACCTGGGTCACCTCTGTGTTTGGAATGATGGCTGTCGTCTAAGAGGGCCCCTGCAGCCACTCCC TGGCACCTGCCCAGCCCCTGAAATCCGAGCGATTGAGCCTCTGAGTGGCCCCTTGGACGGTGGGACTTTG CTGACCATCCGTGGCAGGAACTTGGGCCGTCGGCTCAGTGATGTGGCACATGGTGTGTGGATTGGCAGTG TGGCCTGTGAACCCCTGGCTGACAGATACACCGTTTCAGAGGAGATCGTGTGTGCCACAGGGCCTGCCGC AGGGGCCTTCTCAGACGTGGTAACGGTGAACGTCTCCAAAGAAGGCAGGTCTCGGGAACAGTTCTCCTAT GTGCTGCCCACAGTCCACTCACTGGAGCCTTCCATGGGCCCAAAGGCCGGGGGTACAAGGATCACCATTC ACGGCAGTGACCTCAACGTGGGCTCTATGCTCCAGGTCCTGGTGAATGACACGGACCCCTGCACAGATCT TACGCGCACAGCCACCAGCATCACCTGCACTGTGCCAGGGGGTACCCTGCCCTCTCCAGTGCCTGTGTGT GTGCGCTTCGAGAGCCGGGGCTGCGTGCACGGAAACCTCACCTTCTGGTACATGCAGAACCCAGTCATCA CAGCCATCAGCCCAGGCCGCAGCCCTGTCAGTGGCGGCAGGACCATCACTGTGGCTGGCGAACGCTTCCA CATGGTGCAGAATGTATCAATGGCTGTACACCACATTGGCCGGGAGCCCACGTTCTGCAAGGTTCTCAAC TCCACACTCATCACCTGCCCATCTCCTGGAGCCCTGAGCAATGCTTCGGCGCCTGTAGACTTCTTCATCA ATGGCCGGGCATATGCAGACGAGGCAGCCGAGGAGCTGCTGGACCCTGCAGAGGCACAGAGGGGCAGCCG GTTCCGCCTAGACTACCTCCCCAACCCACAGTTCTCCACAGCCAAGAGGGAGAAGTGGATCAAACATCAC CCAGGAGAGCCGCTCACCCTCGTCATCCATAAGGAGCAAGACAGCCTGGGGCTGGAGAGCCATGAGTACC ACATCAAGATTGGCCAGGTGTCCTGCGACATCCAAATCATCTCAGACAGAGTCATCCACTGCTCAGTCAA TGAGTCGCTGGGCACGGCTGAAGGACAGCTGCCCATCACAATCCAGGTGGGGAACTTCAACCAGACCATC GCCACACTGCAACTGGGGGGCAGCGAGACGGCCATTGTGGTGTCCATCGTCATCTGCAGTGTCCTGTTGC TGCTGTCTGTGGTTGCTCTGTTCGTCTTCTGCACCAAGAGCCGCCGTGCCGAGCGCTACTGGCAGAAGAC CCTGCTGCAGATGGAAGAGATGGAGTCTCAGATCCGAGAGGAGATCCGTAAAGGCTTTGCGGAGCTGCAG ACAGACATGACGGATCTCACCAAGGAGCTGAACCGCAGCCAGGGCATCCCCTTCTTGGAGTACAAGCACT TCGTGACTCGAACCTTCTTCCCCAAGTGCTCTTCCCTCTATGAAGAGCGGTATGTGCTGCCCTCGAAGAC CCTCAACTCCCAGGGTGGCTCCCCGCCACAGGAAACCCACCCACTGCTGGGAGAGTGGAACATCCCTGAA CACTGTCGGCCCAGCATGGAGGAGGGGATCAGCCTGTTCTCCTCACTGCTCAACAACAAGCACTTCCTCA TCGTCTTCGTCCATGCTCTGGAGCAGCAGAAGGACTTCGCAGTGCGTGACAGGTGCAGCCTGGCGTCCCT GCTGACCATCGCGCTGCACGGCAAGCTGGAGTACTATACGAGCATCATGAAGGAGCTGCTCGTGGACCTC ATCGACGCCTCGGCGGCCAAGAACCCCAAGCTCATGTTGCGGCGCACGGAGTCTGTGGTGGAGAAGATGC TTACCAACTGGATGTCCATCTGCATGTACGGCTGCCTGAGGGAGACAGTAGGTGAGCCGTTCTTCCTGCT GTTGTGTGCCATCAAGCAGCAGATCAACAAAGGCTCCATCGACGCCATCACAGGCAAAGCCCGCTACACA CTCAACGAGGAGTGGCTGCTGAGGGAGAACATTGAGGCCAAGCCCCGGAACTTGAACGTGTCCTTCCAGG GCTGTGGGATGGACTCCCTCAGCGTGCGGGCCATGGACACCGACACGCTGACGCAGGTGAAGGAGAAGAT CCTGGAAGCCTTCTGCAAGAACGTCCCCTACTCACAGTGGCCGCGGGCGGAGGACGTGGACCTTGAATGG TTTGCCTCGAGTACCCAGAGCTACGTCCTCCGGGACCTGGATGACACATCAGTGGTGGAGGACGGCCGTA AGAAACTGAACACACTGGCCCACTACAAGATACCTGAGGGCGCCTCCCTAGCCATGAGCCTCACAGACAA GAAGGACAGTACCCTGGGCAGAGTGAAAGACTTGGACACAGAAAAGTATTTCCATTTGGTGCTACCTACG GATGAGCTGGTAGAGCCTAAGAAATCTCACCGGCAGAGCCACCGCAAGAAAGTATTGCCAGAGATCTACC TGACCCGCCTGCTGTCCACCAAGGGCACGCTGCAGAAGTTCCTAGATGACCTGTTCAAGGCTATCCTGAG CATCCGAGAGGACAAGCCCCCGCTGGCTGTCAAGTATTTCTTTGACTTCCTAGAGGAACAGGCGGAGAAG AGAGGCATCTCCGACCCTGACACCCTGCATATCTGGAAGACCAACAGCCTTCCCCTGCGCTTCTGGGTGA ACATCTTAAAAAATCCCCAGTTTGTCTTCGACATAGAGAAGACGGACCACATCGACGCCTGCCTGTCTGT CATCGCACAGGCCTTCATCGATGCCTGCTCCATCTCTGACCTGCAGCTGGGCAAGGACTCACCCACCAAC AAGCTTCTGTACGCGAAGGAGATCCCTGAGTACCGGAAGACCGTACAGCGCTATTATAAACAGATCCAAG ACATGACGCCGCTCAGCGAGCAGGAAATGAACGCACACCTGGCCGAGGAGTCTCGGAAATACCAGAATGA GTTCAACACAAACGTGGCCATGGCTGAGATTTATAAATATGCTAAGAGGTATCGACCACAGATCATGGCT GCCCTGGAGGCCAACCCCACAGCCCGCAGGACCCAGCTACAGCACAAGTTTGAACAGGTGGTGGCTCTGA TGGAAAACAATATCTATGAGTGTTACAGCGAGGCCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_026376
Insert Size	5778 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_026376.3, NP_080652.2
RefSeq Size	6913 bp
RefSeq ORF	5778 bp
Locus ID	67784
UniProt ID	Q3UH93
Gene Summary	Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Mediates anti-angiogenic signaling in response to SEMA3E. Required for normal development of the heart and vasculature.[UniProtKB/Swiss-Prot Function]

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