Marchf8 (NM_001302384) Mouse Untagged Clone
CAT#: MC226797
42071 (untagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8), transcript variant 3
CNY 2,850.00
货期*
3周
规格
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 1300017E09Rik; M; Marc; MARCH-VIII; March8; Mir |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC226797 representing NM_001302384
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATGCCATTGCACCAGATCTCTGCCATCCCTTCTCAGGATGCCATTTCTGCTAGAGTCTACAGAA GCAAGACCAAAGATAAGGAGCAGAATGAGAAGACTTTGGGACATTCCATGAGTCATCCAAGCAACATTTC TAAGGCTGGGAGTAGTCCTCCATCCACGACGGCTCCAGTGTCTGCCTTCTCTCGCACTTCTGTCACACCA TCCAACCAGGACATCTGCAGGATCTGCCACTGTGAAGGGGATGACGAGAGCCCTCTGATCACCCCCTGTC ACTGCACAGGGAGCCTCCATTTCGTGCATCAGGCTTGCCTGCAGCAGTGGATCAAGAGTTCTGACACACG CTGCTGTGAACTCTGCAAGTACGAGTTCATCATGGAGACCAAGCTGAAACCTTTGAGGAAATGGGAGAAG TTGCAGATGACTGCCAGTGAGCGCAGGAAGATCATGTGCTCAGTGACCTTCCATGTCATTGCTATCACCT GTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGCACAGCAGAGGAAATCAAGCAGGGTCAGGTAACAGG AATCCTAGAGTGGCCTTTCTGGACGAAGCTGGTAGTTGTGGCCATCGGCTTCACTGGAGGACTTCTCTTT ATGTATGTTCAGTGCAAGGTGTACCTACAGTTATGGAAAAGACTCAAGGCTTACAATAGAGTGATCTATG TTCAGAACTGTCCAGAAACAAGTAAAAAGAATATTTTTGAAAAGTCTGCACTTACAGAGCCCACCCTTGA AAATAAAGAAGGACATGGAATGTGTCATTCCACCACAAATTCTTCTTGCACAGAGCCTGAAGACACTGGA GCAGAAATTATTAACGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001302384 |
Insert Size | 861 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001302384.1, NP_001289313.1 |
RefSeq Size | 4379 bp |
RefSeq ORF | 861 bp |
Locus ID | 71779 |
UniProt ID | Q9DBD2 |
Gene Summary | The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1). |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR229008 | 42071 (myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 8 (March8), transcript variant 3 |
CNY 2,400.00 |
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