Pitx2 (NM_001287048) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9430085M16Rik; Brx1; Brx1b; Munc30; Otlx2; Ptx2; Rieg
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226815 representing NM_001287048 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGTCCTAGCTCCTGGCCAGCCCCGCTCCTTGGACTCCTCCAAACATAGACTGGAGGTGCATA CAATCTCCGATACTTCCAGCCCTGAAGTCGCAGAGAAAGATAAGGGCCAGCAAGGAAAGAATGAGGATGT GGGCGCCGAGGACCCGTCCAAGAAGAAGCGGCAACGCCGGCAGAGGACTCATTTCACTAGCCAGCAGCTG CAGGAGCTGGAAGCCACTTTCCAGAGAAACCGCTACCCAGACATGTCCACTCGCGAAGAAATCGCCGTGT GGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGCCGGGCCAAATGGAGAAAGCGGGA ACGCAACCAGCAGGCCGAGCTGTGCAAGAATGGCTTTGGGCCGCAGTTCAACGGGCTCATGCAGCCCTAC GATGACATGTACCCCGGCTATTCGTACAACAATTGGGCTGCCAAGGGCCTCACGTCAGCGTCTCTGTCCA CCAAGAGCTTCCCCTTCTTCAACTCCATGAACGTCAATCCCCTGTCCTCTCAGAGTATGTTTTCCCCGCC CAACTCCATCTCATCTATGAGTATGTCGTCCAGCATGGTGCCCTCCGCGGTGACCGGCGTCCCGGGCTCC AGCCTCAATAGCCTGAATAACTTGAACAACCTGAGCAGCCCGTCGCTGAATTCCGCGGTGCCCACGCCCG CCTGTCCTTACGCGCCGCCGACTCCTCCGTACGTTTATAGGGACACATGTAACTCGAGCCTGGCCAGCCT GAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGCCTCCAACCTGAGT GCTTGCCAGTATGCAGTCGACCGGCCGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001287048
Insert Size	873 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287048.1, NP_001273977.1
RefSeq Size	2317 bp
RefSeq ORF	873 bp
Locus ID	18741
Gene Summary	Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform Ptx2c is involved in left-right asymmetry the developing embryo.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The encoded protein (isoform e, also known as PITX2Cbeta) has a distinct N-terminus and is longer than isoform a. Translation begins at a downstream in-frame start codon compared to the AUG used in this transcript to produce PITX2Calpha (PMID 18373856).

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