Kcnj11 (NM_001204411) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Kir6.2; mBIR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226907 representing NM_001204411 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTGGTGGCTCATCGCCTTCGCCCACGGTGACCTGGCCCCCGGAGAGGGCACCAATGTGCCCTGCG TCACAAGCATCCACTCCTTTTCATCTGCCTTCCTTTTCTCCATCGAGGTCCAGGTGACCATTGGTTTCGG CGGGCGCATGGTGACAGAGGAATGTCCCCTGGCCATCCTCATTCTCATTGTGCAGAATATCGTCGGGCTG ATGATCAACGCCATCATGCTGGGCTGCATCTTCATGAAAACGGCCCAGGCCCATCGGCGGGCAGAAACCC TCATCTTCAGCAAGCATGCTGTGATCACCCTGCGCCATGGCCGCCTGTGCTTCATGCTGCGCGTAGGGGA CCTCCGAAAGAGCATGATCATTAGCGCCACCATCCACATGCAGGTGGTGCGCAAGACCACCAGCCCCGAG GGCGAAGTTGTGCCTCTCCACCAGGTAGACATCCCCATGGAGAATGGCGTGGGTGGTAACGGCATCTTCC TGGTGGCCCCACTCATCATCTACCACGTCATCGACTCCAACAGCCCGCTCTACGACCTGGCTCCTAGTGA CCTGCACCACCACCAGGACCTGGAGATCATTGTCATCTTGGAAGGCGTGGTAGAAACCACGGGCATCACC ACCCAGGCCCGCACCTCCTACCTAGCTGACGAGATTCTATGGGGGCAGCGCTTTGTCCCCATTGTGGCCG AGGAGGACGGCCGCTATTCTGTGGACTACTCCAAATTTGGTAACACCATTAAAGTGCCCACACCACTCTG CACAGCCCGCCAGCTTGATGAGGACCGCAGTCTGCTGGATGCCCTGACCCTCGCCTCGTCGCGGGGGCCC CTGCGCAAGCGCAGTGTGGCTGTGGCGAAGGCCAAGCCCAAGTTTAGCATCTCTCCAGATTCCTTGTCCT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204411
Insert Size	912 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204411.1, NP_001191340.1
RefSeq Size	2667 bp
RefSeq ORF	912 bp
Locus ID	16514
Gene Summary	This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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