Il1rl1 (NM_001294171) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	DER4; Fit-1; Ly84; St2; St2-rs1; ST2L; T1; T1/ST2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227110 representing NM_001294171 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGACAGACAGAGAATGGGACTTTGGGCTTTGGCAATTCTGACACTTCCCATGTATTTGACAGTTA CGGAGGGCAGTAAATCGTCCTGGGGTCTGGAAAATGAGGCTTTAATTGTGAGATGCCCCCAAAGAGGACG CTCGACTTATCCTGTGGAATGGTATTACTCAGATACAAATGAAAGTATTCCTACTCAAAAAAGAAATCGG ATCTTTGTCTCAAGAGATCGTCTGAAGTTTCTACCAGCCAGAGTGGAAGACTCTGGGATTTATGCTTGTG TTATCAGAAGCCCCAACTTGAATAAGACTGGATACTTGAATGTCACCATACATAAAAAGCCGCCAAGCTG CAATATCCCTGATTATTTGATGTACTCGACAGTACGTGGATCAGATAAAAATTTCAAGATAACGTGTCCA ACAATTGACCTGTATAATTGGACAGCACCTGTTCAGTGGTTTAAGAACTGCAAAGCTCTCCAAGAGCCAA GGTTCAGGGCACACAGGTCCTACTTGTTCATTGACAACGTGACTCATGATGATGAAGGTGACTACACTTG TCAATTCACACACGCGGAGAATGGAACCAACTACATCGTGACGGCCACCAGATCATTCACAGTTGAAGAA AAAGGCTTTTCTATGTTTCCAGTAATTACAAATCCTCCATACAACCACACAATGGAAGTGGAAATAGGAA AACCAGCAAGTATTGCCTGTTCAGCTTGCTTTGGCAAAGGCTCTCACTTCTTGGCTGATGTCCTGTGGCA GATTAACAAAACAGTAGTTGGAAATTTTGGTGAAGCAAGAATTCAAGAAGAGGAAGGTCGAAATGAAAGT TCCAGCAATGACATGGATTGTTTAACCTCAGTGTTAAGGATAACTGGTGTGACAGAAAAGGACCTGTCCC TGGAATATGACTGTCTGGCCCTGAACCTTCATGGCATGATAAGGCACACCATAAGGCTGAGAAGGAAACA ACCAAGTAAGGAGTGTCCCTCACACATTGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001294171
Insert Size	1014 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001294171.1, NP_001281100.1
RefSeq Size	2904 bp
RefSeq ORF	1014 bp
Locus ID	17082
UniProt ID	P14719
Gene Summary	Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP (PubMed:18450470, PubMed:17675517). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate 5' terminal exon, lacks several exons, and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Variants 2 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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