Camk1d (NM_001290374) Mouse Untagged Clone
CAT#: MC227115
Camk1d (untagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d), transcript variant 2
CNY 3,230.00
货期*
3周
规格
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | A630059D12Rik; CaMKIdelta; CKLiK; E030025C11Rik; mCKLiK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC227115 representing NM_001290374
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGAGAACGGCGAGAGCAGCTCCTCCTGGAAAAAGCAAGCAGAAGACATTAAGAAGATCTTCG AGTTCAAGGAGACCCTCGGAACTGGGGCCTTTTCTGAAGTTGTTTTAGCCGAGGAGAAAGCTACTGGGAA GCTCTTCGCAGTGAAGTGCATCCCGAAGAAGGCGCTGAAGGGCAAGGAGAGCAGCATCGAGAACGAGATT GCCGTGCTTAGAAATGTGTCTGGTGGAGAACTCTTCGATCGGATAGTGGAGAAGGGGTTTTACACAGAGA AAGATGCCAGCACTCTCATCCGCCAGGTCCTGGATGCCGTATACTATCTCCACAGAATGGGCATTGTCCA CAGGGACCTCAAGCCGGAGAATCTCTTATACTACAGTCAAGACGAGGAGTCCAAAATAATGATCAGTGAC TTTGGCTTGTCGAAAATGGAGGGCAAAGGAGATGTGATGTCCACGGCCTGCGGGACCCCAGGCTATGTTG CTCCGGAAGTTCTCGCCCAGAAACCGTACAGCAAAGCTGTGGACTGCTGGTCCATCGGGGTGATCGCCTA TATCTTGCTCTGTGGTTACCCTCCTTTTTATGATGAAAATGACTCGAAGCTGTTTGAACAGATCCTCAAG GCAGAATATGAGTTTGATTCCCCCTACTGGGATGACATCTCCGACTCTGCCAAAGACTTCATTCGGAATC TGATGGAGAAAGACCCAAATAAAAGATACACTTGTGAGCAGGCAGCTCGACACCCATGGATTGCTGGTGA CACAGCCCTTAGCAAAAACATTCACGAATCTGTCAGTGCCCAGATCCGGAAGAATTTTGCAAAGAGCAAA TGGAGACAAGCGTTTAACGCCACGGCAGTCGTGAGACATATGCGGAGGCTCCAGCTTGGCAGCAGCCTGG ACAGTTCAAATGTCTGGCACCTTCCACGCTCTGTAGTTTCCTTTCTTCTTCGTCGGGGGTCGCAGGAGTC GGAGCTGAGAGGAGACCCAGGCCCACCACTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001290374 |
Insert Size | 1014 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001290374.1, NP_001277303.1 |
RefSeq Size | 7108 bp |
RefSeq ORF | 1014 bp |
Locus ID | 227541 |
UniProt ID | Q8BW96 |
Gene Summary | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta (By similarity). Isoform 1 but not isoform 2 activates CREB1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, and uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR229326 | Camk1d (myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase ID (Camk1d), transcript variant 2 |
CNY 3,330.00 |
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