Pus7 (NM_001289781) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C330017I15Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228598 representing NM_001289781 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATGACAAGCACGTCCCTGAAACGTGGGTGCCTTGTTGTGGAAGATAATGACAGTGTGACCCCAC ACGACGAAACAAAGAAACAGAAGGTGTCCGAGGGCTGTCTGACCAGCAGTCAGGATGGGGTAGAGAATGA CGGTCTACACCGCAGTGAGAACGAGCCTGGGCCTCCAGAAGCTGAAAGCACCGTGAAAGATGACGAGAAT TCCAGCGCTCAGGTGCAAGAGGAAGAGGAGGAGGAGGAGGAAGAAGACGGCCTTTCAGAAGCAGGGGAAG AGGAAGAAGCAGAGAGTTTTGCAGACATGATGAAACATGGGCTCACAGAACTTGATGTGGGCATCTGCAA GTTCGTGAGTTCCCATCACGGCTTCTCGGGGATCTTAAAAGAAAGATACTCTGACTTTGTCGTTCACGAA ATCGGGAAAGATGGACGGATCAGCCATTTGGATGACTTATCTGTTCCGGTGGATGAAGAGGACCCTCCAG AAGACGCTCTCACTGTCTTGACAGCTGAGGACAGGCAGCAGTTGGAGGAGCTCCAGCTTTTCAAAAATAA AGAAACCAGTGTTGCCATCGAGGTCATTGAGGACACCAAAGAGAAGCGAACAGTCATCCATCAAGCCATT AAATCTCTCTTCCCAGGACTAGAGACAAAGACAGAGGATAGAGAGGGGCGGAAATACATCGTAGCCTACC ATGCAGCTGGCAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCCCGGGGAAGTTACTG CCATTTTGTTCTCTACAAAGAAAACAAAGACACCATGGATGCCATCAATGTTCTATCCAAGTACCTGCGG GTAAAGCCAAACATATTCTCCTATATGGGAACCAAAGACAAAAGGGCAATCACAGTCCAGGAGATTGCTG TTCTCAAAATAAGTGCACAAAGACTTGCCCACCTCAATAAGTGCCTGATGAACTTGAAGCTCGGGAATTT CAGCTATCAGAAAACCCCTCTGAAGTTAGGAGCGCTCCAAGGAAACCACTTCACTGTTGTTCTCAGGAAT ATAACAGGAACTGATGAGCAGGTGCAGCAAGCCATGCAGTCTCTGAGGGAGACCGGCTTCATTAACTACT ATGGCATGCAAAGGTTCGGGACCACAGCTGTTCCAACGTACCAGGTTGGAAGAGCAATTCTACAGAATTC CTGGACAGAAGTCATGGATTTAATACTGAAACCCCGTTCTGGAGCTGAGAAGGGTTACTTGGTGAAATGC AGAGAAGAATGGGCCAAGACCAAAGACCCAGCCTCTGCTCTCAAAAAGCTGCCCGTCAAAAGGTGTGTGG AAGGGCAGCTGCTCCGAGGACTTTCCAGATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAATTCC AAGAAACAATCGCTTAATGTACATCCACAGTTATCAGAGCTACGTGTGGAACACCATGGTGAGCAGAAGA ATTGAGGAGTACGGGCTGAGGCCTGTGCCCGGGGACCTTGTTCTCAAAGGAGCCACGCCCACCTACATTG AGGAAGATGACGTTGACAACTACTCCATCCACGATGTGGTGATGCCTTTGCCTGGGTTTGATGTTATCTA CCCCAAGCATAAAATCAGCGAGGCCTACAGGGAAATGCTTGCTGCAGACAACCTTGATATTGACAACATG AGACACACGATTCGAGATTACTCCTTATCGGGGGCCTACCGAAGGATCATCATCAGGCCTCAGAGCGTCA GCTGGGAAGTTGTTGCATATGATGATCCTAAAATTCCACTTTTCAACACGGATGTGGACAACCTCGAGGG GAAACCACCACCAGTGTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAGATGGACTTTTCTCTTCCTCCT TCTACTTACGCCACCATGGCCATTCGAGAAGTGCTCAAAATGGACACCAGCATCAAGAACCAGACCCAGC TAAATACGAGCTGGCTGCGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289781
Insert Size	1983 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289781.1, NP_001276710.1
RefSeq Size	4258 bp
RefSeq ORF	1983 bp
Locus ID	78697
UniProt ID	Q91VU7
Gene Summary	Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which lacks an internal segment and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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