Clcn4 (NM_001302386) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	clC-4; Clc4-2; Clcn4-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228674 representing NM_001302386 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACTCATTGGGCTGCTGGCAGGTACCTTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGG ACCTCAAGGAGGGGGTCTGTCTGTCCGCATTCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGA GACCACTTTTGAGGACAGGGACAAGTGTCCCCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCA GAGGGCGCCAGCGCTTACATTCTGAATTACTTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGG CTGTCTCCCTGGTACGTGTGTTCGCACCGTATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTT GAGTGGCTTTATCATCAGGGGATACTTGGGGAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTC GTCGTATCCTCTGGCCTGAGCCTTGGCAAAGAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACT TCTTCAGCAGCCTTTTCTCCAAGTATAGCAAGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGC TGCTGCTGGTGTCTCTGTGGCCTTTGGTGCTCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGT TACTACTTTCCCTTGAAAACCTTGTGGAGGTCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCT CCATCAACCCCTTTGGAAATAGCCGCCTGGTTCTCTTTTATGTGGAGTATCATACACCCTGGTACATGGC TGAACTCTTCCCTTTCATCCTGCTTGGAGTCTTTGGGGGTTTATGGGGAACCCTCTTCACACGCTGCAAC ATTGCTTGGTGCAGGAGGCGTAAGACCACCAGGCTGGGCAGGTACCCAGTGTTGGAGGTTATTGCGGTGA CAGCCGTCACCGCCATCGTGGCCTACCCCAATCCCTACACTCGCCAGAGCACCAGTGAGCTCATCTCTGA GCTCTTCAACGATTGTGGGGCTCTCGAGTCTTCTCAGCTCTGTGACTACATCAACGACCCCAACATGACT CGGCCTGTGGATGACATTCCGGACCGGCCGGCTGGGGTTGGAGTTTACACAGCCATGTGGCAGCTGGCCT TGGCACTGATCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATTCCCTCAGGTCTCTTCAT CCCCAGTATGGCTGTCGGAGCCATGGCAGGCCGGATGGTGGGAATCGGTGTGGAGCAGCTGGCCTACCAT CACCATGACTGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGTCACACCAGGGCTTTATG CGATGGTGGGAGCTGCAGCCTGTCTAGGTGGGGTGACTAGGATGACAGTGTCTCTAGTGGTCATTATGTT TGAACTGACTGGAGGTCTGGAGTATATTGTACCCCTGATGGCAGCTGCTGTCACCAGCAAGTGGGTGGCT GATGCCTTTGGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTACCCATTTCTTGATGTGA AGGATGAGTTCACCCACCGTACGCTGGCCACTGATGTGATGCGGCCCCGGAGGGGAGAACCGCCATTATC GGTACTAACCCAGGACAGCATGACTGTGGAGGACGTGGAGACTCTCATCAAGGAGACAGACTACAACGGC TTTCCTGTGCTCGTCTCCAGAGACTCGGAGCGTCTCATCGGGTTTGCCCAGAGGCGGGAGCTAATCTTGG CTATAAAAAATGCCAGGCAGAGGCAAGAGGGCATTGTGAGCAATTCCATCATGTACTTCACAGAGGAGCC TCCTGAGCTGCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTTTGAACCTGAGCCCTTTCACG GTCACAGATCACACCCCCATGGAGACGGTGGTGGACATTTTCCGGAAACTGGGGCTCCGACAATGCCTGG TGACACGGAGTGGGAGACTTCTTGGGATCATCACAAAAAAGGATGTTCTGAGACACATGGCCCAGATGGC AAACCAGGACCCTGAATCCATCATGTTTAATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302386
Insert Size	2064 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302386.1, NP_001289315.1
RefSeq Size	4169 bp
RefSeq ORF	2064 bp
Locus ID	12727
Gene Summary	Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) is shorter at the 5' end, lacks an alternate exon in the 5' coding region and uses a downstream start codon, compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.

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