Clcn4 (NM_001302387) Mouse Untagged Clone

CAT#: MC228753

Clcn4 (untagged) - Mouse chloride channel 4-2 (Clcn4-2), transcript variant 3



  "NM_001302387" in other vectors (1)

CNY 6,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Clc4-2; Clcn4-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228753 representing NM_001302387
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTCCTCGAGGAGCCCTTCCCTGACGTGGGGACCTACGAGGACTTCCACACCATAGACTGGCTGA
GGGAAAAGTCCCGGGATACCGACAGACATAGGAAGATCACCAGCAAAAGTAAGGAGTCTATTTGGGAGTT
CATCAAGAGCCTGCTGGACGCGTGGTCGGGATGGGTGGTGATGCTACTCATTGGGCTGCTGGCAGGTACC
TTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGGACCTCAAGGAGGGGGTCTGTCTGTCCGCAT
TCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGAGACCACTTTTGAGGACAGGGACAAGTGTCC
CCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCAGAGGGCGCCAGCGCTTACATTCTGAATTAC
TTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGGCTGTCTCCCTGGTACGTGTGTTCGCACCGT
ATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTTGAGTGGCTTTATCATCAGGGGATACTTGGG
GAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTCGTCGTATCCTCTGGCCTGAGCCTTGGCAAA
GAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTATAGCA
AGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGCTGCTGCTGGTGTCTCTGTGGCCTTTGGTGC
TCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGTTACTACTTTCCCTTGAAAACCTTGTGGAGG
TCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCTCCATCAACCCCTTTGGAAATAGCCGCCTGG
TTCTCTTTTATGTGGAGTATCATACACCCTGGAGGCGTAAGACCACCAGGCTGGGCAGGTACCCAGTGTT
GGAGGTTATTGCGGTGACAGCCGTCACCGCCATCGTGGCCTACCCCAATCCCTACACTCGCCAGAGCACC
AGTGAGCTCATCTCTGAGCTCTTCAACGATTGTGGGGCTCTCGAGTCTTCTCAGCTCTGTGACTACATCA
ACGACCCCAACATGACTCGGCCTGTGGATGACATTCCGGACCGGCCGGCTGGGGTTGGAGTTTACACAGC
CATGTGGCAGCTGGCCTTGGCACTGATCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATT
CCCTCAGGTCTCTTCATCCCCAGTATGGCTGTCGGAGCCATGGCAGGCCGGATGGTGGGAATCGGTGTGG
AGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGT
CACACCAGGGCTTTATGCGATGGTGGGAGCTGCAGCCTGTCTAGGTGGGGTGACTAGGATGACAGTGTCT
CTAGTGGTCATTATGTTTGAACTGACTGGAGGTCTGGAGTATATTGTACCCCTGATGGCAGCTGCTGTCA
CCAGCAAGTGGGTGGCTGATGCCTTTGGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTA
CCCATTTCTTGATGTGAAGGATGAGTTCACCCACCGTACGCTGGCCACTGATGTGATGCGGCCCCGGAGG
GGAGAACCGCCATTATCGGTACTAACCCAGGACAGCATGACTGTGGAGGACGTGGAGACTCTCATCAAGG
AGACAGACTACAACGGCTTTCCTGTGCTCGTCTCCAGAGACTCGGAGCGTCTCATCGGGTTTGCCCAGAG
GCGGGAGCTAATCTTGGCTATAAAAAATGCCAGGCAGAGGCAAGAGGGCATTGTGAGCAATTCCATCATG
TACTTCACAGAGGAGCCTCCTGAGCTGCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTTTGA
ACCTGAGCCCTTTCACGGTCACAGATCACACCCCCATGGAGACGGTGGTGGACATTTTCCGGAAACTGGG
GCTCCGACAATGCCTGGTGACACGGAGTGGGAGACTTCTTGGGATCATCACAAAAAAGGATGTTCTGAGA
CACATGGCCCAGATGGCAAACCAGGACCCTGAATCCATCATGTTTAATTAG


ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-NotI     
ACCN NM_001302387
Insert Size 2151 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001302387.1, NP_001289316.1
RefSeq Size 4231 bp
RefSeq ORF 2151 bp
Locus ID 12727
Gene Summary Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. It encodes isoform 3 which is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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