Fer (NM_001286415) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV082135; C330004K01Rik; Fert; Fert2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228884 representing NM_001286415 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATTTGGGAGTGACCTGAAGAACTCACAGGAAGCTGTGTTAAAGTTGCAAGACTGGGAACTACGGT TGCTGGAGACAGTGAAGAAATTTATGGCTCTGAGAATAAAGAGTGATAAAGAATATGCATATACTCTACA GAATCTTTGTAATCAAGTTGATAAAGAAAGCACTGTGCAAGTGAATTATGTCAGCAACGTGTCCAAGGTC ACAAAGACAGAATTGGAGAAATTAAAATCCAGCTATCGACAATTAATAAAAGAAATGAATTCTGCCAAAG AGAAATATAAAGAAGCCTTAGCGAAAGGGAAGGAAACAGAAAAGGCCAAGGAGCGCTATGACAAAGCTAC AATGAAGCTTCATATGCTGCATAATCAGTATGTGTTGGCACTGAAGGGGGCACAGCTCCATCAGAGCCAG TACTATGATACCACACTTCCTCTGCTGCTGGACTCCGTGCAAAAGATGCAAGAAGAAATGATAAAAGCAC TAAAAGGTATATTTGATGACTATAGCCAGATAACCAGCCTTGTTACAGAGGAAATAGTGAATGTCCATAA AGAGATTCAAATGTCTGTTGAACAGATAGACCCTAGCACCGAATATAATAATTTCATAGATGTTCACAGA ACAACAGCTGCTAAAGAACAAGAAATCGAATTTGATACTTCCCTATTAGAAGAAAATGAAAATCTTCAGG CAAATGAGATCATGTGGAATAATTTAACAGCAGACAGTTTGCAAGTCATGTTGAAGACTTTAGCGGAAGA GCTCACACAGACCCAGCAGATGCTTTTACACAAGGAGGCGGCCGTCCTGGAGCTAGAGAAGAGGATTGAA GAATCTTTTGAGACCTGTGAAAAGAAGTCTGACATTGTGCTTCTTCTGGGCCAAAAACAGGCACTTGAAG AGTTGAAACAGTCAGTCCAGCAGTTGCGATGCACTGAGGCAAAGTGTGCAGCACAGAAAGCTTTGCTGGA GCAAAAAGTACAGGAAAATGATGGGAAAGAACCACCTCCTGTGGTGAATTACGAAGAAGACGCGCGGTCA GTCACATCCATGGAAAGAAAGGAGAGGCTATCCAAATTTGAGTCTATTCGTCATTCAATTGCTGGGATAA TTAAGTCTCCAAAGTCGGTACTCGGATCTTCAACACAGGTGTGTGATGTGATCTCTGTGGGTGAGAGGCC CCTGGCGGAGCATGACTGGTACCATGGTGCCATCCCCAGGATAGAGGCACAGGAACTGCTGAAGCAGCAA GGAGACTTCCTGGTGCGGGAGAGCCATGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGACGGAC AAAGGAGGCACTTTATCATACAATTTGTCGATAATCTGTATCGATTCGAGGGCACCGGGTTTTCAAACAT TCCCCAGCTTATAGATCACCACTTCAATACAAAGCAAGTCATCACCAAGAAGTCTGGGGTGGTTCTGCTC AACCCCATCCCAAAGGATAAGAAATGGGTTCTCAATCATGAAGATGTTTCATTGGGAGAATTACTGGGCA AGGGGAATTTTGGTGAAGTGTATAAGGGCACACTAAAGGATAAAACTCCTGTTGCCATTAAAACGTGCAA GGAAGACCTTCCTCAGGAATTAAAAATAAAGTTTCTACAGGAAGCCAAAATTCTGAAGCAATATGATCAT CCCAATATTGTCAAACTGATAGGCGTGTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTCC CAGGGGGTGATTTTCTGACCTTCCTGAGGAAGAGGAAGGACGAGCTGAAGCTGAAGCAGTTGGTGAGATT TTCCTTGGACGTTGCTGCTGGCATGTTGTATCTCGAGAGCAAGAACTGCATTCACAGGGACCTGGCTGCA CGGAACTGCCTGGTGGGTGAAAATAATACTCTGAAAATCAGTGACTTTGGAATGTCTCGGCAAGAAGACG GTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTCCTATTAAATGGACAGCACCGGAAGCTCTTAATTA TGGGAGATACAGTTCTGAAAGTGACGTGTGGAGCTTCGGCATCCTCCTCTGGGAGACCTTCAGCCTAGGA GTCTGCCCGTACCCTGGAATGACAAACCAGCAAGCGCGAGAGCAAGTGGAGAGAGGATACCGGATGTCAG CCCCACAGAACTGTCCAGAGGAGGTTTTTACAATCATGATGAAGTGTTGGGATTACAAGCCTGAAAACCG CCCTAAGTTCAACGACCTTCACAAAGAGCTCACTGTCATCAAGAAGATGATCACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286415
Insert Size	2298 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286415.1, NP_001273344.1
RefSeq Size	2948 bp
RefSeq ORF	2298 bp
Locus ID	14158
UniProt ID	P70451
Gene Summary	Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR exon, and lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (c) lacks an internal segment, compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.