Asap1 (NM_001276462) Mouse Untagged Clone

CAT#: MC229391

Asap1 (untagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 3



  "NM_001276462" in other vectors (1)

CNY 10,740.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal ASAP1 phospho Y782 antibody (Phospho-specific)
    • 100 ug

CNY 8,025.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229391 representing NM_001276462
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGACCAGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGT
CCAGCTTCACCACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGA
TAGAACAGCCCTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAA
AATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACC
TTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCA
GGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGA
GATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGA
AAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAAT
GGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACC
AAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATG
GCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCA
AACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGAT
CCGAAAGAAGTAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTGACTCTCAGAGCCGGCAAGGTGGGT
ACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAG
TGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCAC
GCAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAAGTGAAACCGAATGCTGAGGACA
AGAAGTCTTTTGACCTGATATCACATAATCGAACATATCACTTTCAAGCAGAAGATGAGCAGGATTATAT
AGCGTGGATATCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGC
ACAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCCATCATCGAGGATGTACAGCGGCTCCCTGGGAATG
ACATCTGCTGTGACTGCGGCTCATCAGAACCCACGTGGCTTTCAACCAACTTGGGTATTTTGACCTGTAT
AGAATGTTCCGGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAA
TTAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAA
ATTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACCGTGCGTAAAGAGTACATCACTGC
AAAGTATGTCGACCACCGCTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTT
GAGGCCATCAAGTCCAGGGACTTACTTGCATTAATCCAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGC
CACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAGCCCTTCATCTTGCCGTCAGGACCGCAGACCAGAC
ATCTCTCCATCTGGTTGATTTCCTTGTACAAAACTGTGGGAACCTGGATAAGCAAACGTCTGTGGGGAAT
ACAGTTCTGCACTATTGCAGCATGTACGGCAAGCCGGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCA
CCGTGGACATCGTTAACCAGAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACCCAGTG
TGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAACCCTCATGTCCACGTAGAGTATGAGTGGAAT
CTTCGACAGGACGAGATGGATGAGAGCGATGACGATCTGGATGACAAGCCAAGCCCGATCAAAAAGGAAC
GCTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCAGCATCTCGCCACAGGACAAGCTGGCACTGCCAGG
GTTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGCCTCTACG
AGCACAGATTTGCCCACATCACCCACCAGTGAGGCTCCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTC
CAACTGGCCCACCTTCAACACTCCCTCTAGGCACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAA
GAGGCCTCCTCCCCCACCACCAGGACACAAGAGAACCCTGTCTGACCCTCCCAGCCCACTACCTCACGGG
CCCCCAAACAAAGGCGCAATTCCTTGGGGTAATGATGTGGGCCCATTATCTTCAAGTAAGACGGCCAACA
AGTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCC
TAAACTACCTCAGAAAGTGGCACTAAGGAAGACGGAGACCAGCCATCATCTCTCCCTCGACAGAACCAAC
ATCCCACCTGAGACTTTTCAGAAATCATCACAGTTGACAGAGTTACCCCAAAAGCCACCACTTGGAGAGC
TGCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCCAAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCT
ACCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAG
ATGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGATCTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAG
CCAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGTCACACACCGGGGATCTGTCTCCAAATGTACAGTC
CAGAGATGCCATCCAGAAGCAAGCATCTGAAGACTCCAACGACCTCACACCCACGCTGCCAGAGACACCC
GTACCACTGCCGAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGGGTGAAGACCATTTATGACTGCC
AGGCAGATAATGACGACGAACTCACATTTATTGAGGGGGAAGTGATCATTGTCACCGGGGAAGAGGACCA
GGAGTGGTGGATCGGGCATATCGAAGGACAGCCTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCAC
ATCCTGTCTGACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001276462
Insert Size 3375 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001276462.1, NP_001263391.1
RefSeq Size 6181 bp
RefSeq ORF 3375 bp
Locus ID 13196
UniProt ID Q9QWY8
Gene Summary May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR, uses a downstream start codon, and lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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