Arhgap33 (NM_001289682) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW324378; NOMA-GAP; Snx26; TCGAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229550 representing NM_001289682 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCAGGCACAGAAACAGTCAGATCCCATCCTGCCTTGGGGAGCTTCATGGGCTGGCAGGGGACAGA CTCTGAGGGCCCGCAGCACTGACAGCCTGGATGGCCCAGGGGAGGGCTCAGTGCAGCCCGTACCCACTAC TGGGGGGCCGGGTACAAAGGGGAAGCCTGGGAAGAGGCTCTCAGCTCCTCGAGGCCCTTTCCCCCGGCTG GCTGACTGTGCCCATTTCCACTATGAGAATGTTGACTTTGGCCACATTCAGCTCCTGCTGTCTCCAGAGC GTGAGGGCCCCAGCCTCTCTGGAGAAAATGAGCTGGTCTTTGGGGTACAGGTGACCTGTCAGGGCCGCTC CTGGCCAGTCCTCCGGAGCTATGACGATTTCCGTTCTCTGGATGCCCATCTCCACCGGTGCATATTTGAC CGGAGATTTTCCTGCCTCCCAGAGCTCCCTCCACCTCCAGAGGGTACCAGGGCTGCCCAGATGCTGGTAC CGCTGCTGCTGCAGTACCTGGAGACCCTGTCAGGACTGGTGGACAGTAACCTCAACTGTGGACCTGTGCT CACTTGGATGGAGCTGGACAACCATGGCCGGCGCCTGCTCCTCAGTGAGGAAGCCTCCCTCAATATCCCT GCTGTGGCTGCTGCCCACGTGGTCAAGCGGTACACTGCCCAGGCGCCAGATGAGCTGTCCTTTGAGGTGG GAGACATTGTCTCAGTGATCGACATGCCACCTACAGAGGATCGGAGCTGGTGGCGGGGCAAACGGGGCTT CCAGGTTGGTTTCTTCCCCAGTGAGTGTGTGGAACTCTTCACGGAGAGGCCAGGGCCTGGCCTAAAGGCA GATGCTGACAGTCCCCTGTGTGGCATCCCAGCTCCCCAGGGCATCTCTTCTCTGACCTCAGCTGTGCCCC GGCCACGTGGGAAGCTGGCAGGACTCCTCCGCACCTTCATGCGCTCTCGTCCTTCTCGCCAGCGGCTGCG CCAACGGGGAATCCTGCGGCAGAGGGTGTTTGGTTGTGATCTTGGAGAGCACCTCAGCAACTCAGGCCAA GACGTGCCCCAAGTGCTGCGCTGCTGCTCTGAGTTTATTGAGGCCCATGGTGTGGTGGATGGAATCTACA GGCTCTCTGGCGTGTCCTCCAACATCCAGAGGCTTCGGCATGAGTTTGATAGTGAGAGGATCCCTGAGCT GTCTGGCCCCGCCTTCCTACAAGATATCCACAGTGTGTCCTCCCTCTGCAAGCTCTACTTCCGGGAACTG CCCAACCCCTTGCTCACCTACCAGCTCTATGGGAAGTTCAGTGAGGCTATGTCAGTGCCTGGGGAGGAAG AACGCCTAGTGCGAGTGCATGACGTCATCCAGCAGCTGCCTCCACCACACTACAGGACCCTGGAGTACCT GCTGAGGCACCTGGCCCGCATGGCAAGACACAGCGCTAACACCAGCATGCATGCCCGCAACCTGGCCATT GTCTGGGCACCCAACCTGCTACGGTCTATGGAACTGGAGTCGGTGGGGCTGGGTGGGGCAGCCGCCTTCC GGGAGGTCCGGGTGCAGTCGGTGGTGGTGGAGTTCCTGCTTACCCACGTGGAGGTTCTGTTCAGCGATAC GTTCACCTCTGCTGGCCTGGACCCTGCAGGTCGCTGCCTCCTTCCCAGACCCAAGTCCCTTGCGGGGAGC AGTCCCTCCACTCGCCTGCTAACACTGGAAGAAGCCCAGGCACGAACTCAGGGCCGTCTTGGAACACCTA CAGAGCCCACGACTCCTAAGACGCCAGCCTCACCAGTGGAAAGGAGGAAAAGAGAGAGGGCGGAGAAACA AAGGAAGCCAGGAGGTAGCAGCTGGAAGACATTCTTTGCTCTGGGGCGGGGCCCCAGCATACCCCGGAAG AAGCCGCTGCCATGGCTGGGTGGCAGCCGAGCCCCACCACAGCCTTCAGGCAGCAGACCTGACACTGTCA CGCTGAGATCTGCCAAAAGTGAGGAGTCTCTGTCATCCCAGGCCAGCGGGGCTGGCCTCCAGAGGCTGCA CCGGCTGCGACGACCCCACTCCAGCAGCGATGCTTTTCCCGTGGGCCCAGCACCTGCTGGCTCTTGCGAG AGCTTGTCCTCTTCTTCTTCCTCTTCTTCCTCTTCCTCCTCCTCCTCCTCCTCAGAGTCTTCAGCAGGTG GCCTGGGGCCACTCTCTGGCTCCCCCTCACACCGCACCTCAGCCTGGCTAGATGATGGCGATGATCTGGA TTTCAGTCCACCCCGCTGTCTGGAAGGACTTCGGGGACTTGACTTTGATCCCCTTACCTTTCGCTGCAGC AGCCCCACCCCAGGGGACCCTGCACCTCCTGCCAGCCCAGCACCTCCAGCCTCAGCCTCTGCCTTCCCAC CTCGGGCAACCCCACAGGCTCTGTCACCCCATGGACCCACTAAGCCTGCTTCACCCACTGCCCTGGACAT CTCAGAGCCCCTGGCTGTATCAGTACCACCTGCTGTCCTGGAACTACTGGGAGCTGGAGGAACGCCTGCC TCAGCCACCCCAACACCGGCTCTCAGCCCTCATCTCATCCCCCTGCTGCTGCGTGGAGCTGAGGCCCAGC TAAGTGACACCTGTCAGCAGGAGATCAGCAGCAAGCTAGCACCAACCCGGGGAGCTCCAGGCCAGCAAAG TCCTGGCGGCATGGATTCACCGTTATTGCCCCCACCCTTGCCTCTGCTGCGCCCTGGTGGGGCTCCGCCC CCTCCCCCCAAGAACCCAGCTCGTCTCATGGCCCTGGCCTTGGCTGAGCGAGCTCAGCAGGTAGCAGAGC AACAGAGCCAACAAGAGCAGGGGGGCACCCCACCTGCTCCCCACTCCCCCTTTCGACGCTCTCTGTCCCT AGAGGTGGGTGGGGAGCCTGTGGGGACTTCAGGGAGTGGGATACACCCTCCCTCCTTAGCTCACCCAGGT GCCTGGGCTCCAGGTCCCCCACCTTACCTCCCAAGGCAACAGAGTGATGGGAGCCTGGTAAGAAGCCAGC GGCCCTTGGGGACCTCAAGGAGAAGTCCCAGAGGGCCTTCCCAGGTCAGTGCCCATCTCAGGGCAAGCGG GGCTTACAGGGATGCTCCAGAGATGGCAGCCCAGTCACCATGTTCTGTCCCCTCACAGGGTTCTAACCCC AGCTTCTTCTCAACCCCCCGAGAGTGTTTGCCACCTTTCCTTGGCGTCCCCAAACAAGGCTTGTACTCTC TGGGACCCCCATCCTTCCCACCTAGCTCTCCAGCCCCAGTCTGGAGGAACTCTCTGGGTGCCCCCTCAGC ATTGGACAGGGGAGAGAATCTGTACTATGAAATTGGGGTAGGGGAGGGGACCTCCTACTCAGGCCCCAGC CGGTCCTGGAGTCCGTTTCGCTCCATGCCTCCCGACAGGCATAATGCCTCCTATGGTATGCTTGGCCAGT CACCACCGCTCCACAGGTCCCCTGATTTCCTGCTCAGCTACCCGCCTCCCCCCTCCTGCTTTCCCCCAGA GCACCTTACCCACTCAGTTTCACAGCGCCTTGCCCGGCGCCCTACCCGTCCTGAGCCCCTCTATGTCAAC CTAGCCCTAGGGCCCAGGGGCCCTTCACCTGCCTCTTCTTCCTCTTCCTCTCCTCCTGCCCACCCCAGGA GCCGGTCAGATCCTGGGCCTCCTGTTCCCCGCCTCCCTCAGAAACAGCGGGCTCCTTGGGGTCCACATAC TCCCCATCGGGTGCCTGGACCTTGGGGTTCCCCCGAACCTTTTCTGCTCTATAGGCCAGCTCCACCATCC TATGGGAGGGGAGGGGAGGTCCGAGGATCCTTGTACAGAAATGGAGGACATAGAGGAGAGGGGGCTGGTC CCCCTCCTCCTTACCCCACACCCAGCTGGTCCTTACATTCAGAAGGACAGACCAGAAGTTACTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289682
Insert Size	3918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289682.1, NP_001276611.1
RefSeq Size	4439 bp
RefSeq ORF	3918 bp
Locus ID	233071
UniProt ID	Q80YF9
Gene Summary	May be involved in several stages of intracellular trafficking (By similarity). Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode isoform a.

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