Brd4 (NM_001286630) Mouse Untagged Clone

CAT#: MC229579

Brd4 (untagged) - Mouse bromodomain containing 4 (Brd4), transcript variant 3



  "NM_001286630" in other vectors (1)

CNY 13,490.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Brd5; HUNK1; MCAP; WI-11513
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229579 representing NM_001286630
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACCT
CCCAAATGTCTACAACGCAGGCCCAGGCCCAACCCCAGCCAGCAAATGCAGCCAGCACCAATCCTCCACC
CCCAGAGACCTCCAACCCTAACAAGCCCAAGAGACAGACAAACCAACTGCAATATCTGCTCAGAGTGGTG
CTCAAGACACTATGGAAACACCAGTTTGCGTGGCCTTTCCAGCAGCCCGTGGATGCCGTCAAGCTGAACC
TCCCTGATTACTATAAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAA
CTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTATAAC
AAGCCTGGAGATGACATCGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAATGAAC
TGCCTACAGAAGAAACTGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGAGGGAGGAAAGAAACAGG
TAGGGCAGCAAAGCCTGGTGTATCCACGGTACCAAACACAACTCAAGCATCAACTTCTCCGCAGACCCAG
ACGCCTCAGCAGAACCCTCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAGACC
TCATTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCTCAGCCACTTCAGACTCCTTCACCGGTACCCCC
CCAGCCACCACCCCCACCTGCTCCAGTTCCACAGCCTGTGCAGAGTCACCCGCCCATCATTGCGACCACC
CCCCAGCCTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCTACCACCATCGACC
CCATTCATGAGCCACCCTCACTGGCCCCAGAGCCCAAGACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAG
CAGACCTGTGAAGCCTCCAAAGAAGGATGTACCGGACTCACAGCAGCACCCAGGGCCAGAGAAGAGCAGC
AAGATCTCTGAGCAGCTAAAGTGCTGCAGTGGCATCCTCAAGGAGATGTTTGCCAAGAAACATGCTGCCT
ATGCCTGGCCTTTCTACAAGCCTGTGGATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATCAA
ACATCCCATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGAGAGTACAGAGATGCCCAGGAATTT
GGTGCTGATGTCCGATTGATGTTCTCCAACTGCTACAAGTACAACCCCCCTGACCATGAAGTGGTAGCCA
TGGCTCGAAAACTCCAGGATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCCAGT
TGTTACAGTGTCCTCTCCTGCAGTGCCACCCCCTACAAAGGTGGTAGCCCCACCCTCATCTAGTGACAGC
AGCAGCGACAGTTCTTCCGACAGCGACAGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGG
CTGAACTCCAGGAACAGCTCAAGGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGCCCCAGCAGAACAA
ACCAAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAAAAGGAAAAGCACAAAAAGAAAGAAGAAGTGGAG
GAAAATAAAAAAAGCAAAACCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACAGCA
ATGTGAGCAAGAAGGAACCAGTACCCACGAAGACCAAGCCGCCTCCCACATATGAATCAGAAGAGGAGGA
TAAGTGTAAGCCCATGTCTTATGAGGAGAAGCGGCAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAG
AAGCTAGGCCGTGTAGTACACATAATTCAGTCAAGGGAACCATCACTTAAAAACTCCAACCCCGATGAGA
TTGAGATTGACTTTGAGACCCTGAAGCCATCTACACTACGAGAGTTGGAGCGATATGTCACCTCCTGTTT
GCGGAAGAAAAGGAAACCTCAAGCTGAAAAAGTTGACGTGATTGCTGGTTCTTCCAAGATGAAGGGATTC
TCATCCTCTGAGTCGGAGAGCACCAGCGAATCCAGCTCCTCTGACAGTGAAGACTCTGAAACAGAGATGG
CTCCCAAGTCAAAAAAGAAGGGGCACACTGGGAGGGACCAGAAAAAGCATCATCACCATCACCATCCACA
GATGCAGCCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCCCCACCTCCACAGCAGCCTCCTCCACCCCCA
CCTCCGCAGCAGCAGCAGCAGCAACCTCCACCCCCACCACCTCCGCCCTCCATGCCACAGCAGACTGCCC
CAGCGATGAAGTCCTCGCCCCCACCCTTCATCACTGCCCAGGTCCCCGTCCTGGAACCACAGCTGCCAGG
CAGTGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACCTGCCGCAGCCGGAGCTGCCTCCT
CACCTGCCCCAGCCACCTGAGCACAGCACTCCACCCCATCTCAACCAGCATGCTGTGGTCTCTCCTCCAG
CTTTGCACAATGCGCTGCCCCAACAGCCATCTCGGCCCAGTAACCGAGCTGCTGCTCTGCCCCCAAAGCC
TACCCGACCCCCAGCTGTGTCCCCTGCCCTGGCCCAGCCCCCCCTGCTCCCACAACCACCAATGGCTCAG
CCCCCCCAAGTGCTGCTGGAGGATGAAGAGCCACCTGCCCCACCCCTCACCTCCATGCAGATGCAGTTGT
ACCTACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCTTCCGTGAAGGTGCAGTCCCAACC
CCCACCGCCTTTGCCGCCTCCACCCCACCCTTCTGTGCAGCAGCAGCAGCTCCAGCCACAGCCACCGCCA
CCCCCACCTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCTCCACCACGACCAGTTCACTTGC
CATCCATGCCCTTTTCTGCTCATATTCAGCAGCCCCCACCACCCCCAGGACAGCAACCTACTCACCCACC
CCCAGGACAGCAGCCCCCACCACCACAGCCTGCCAAGCCCCAGCAAGTCATCCAGCATCACCCTTCCCCC
CGGCACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAGGCTCCCTCTCCGCTTATGATACATT
CCCCTCAGATGCCACAGTTCCAGAGCCTGACCCATCAGTCTCCTCCCCAGCAAAACGTCCAGCCTAAGAA
GCAGGTAAAGGGCAGGGCTGAGCCACAGCCACCAGGGCCAGTCATGGGCCAAGGCCAGGGATGCCCACCT
GCCTCACCGGCTGCCGTGCCTATGCTGTCCCAGGAGCTGCGACCACCTTCAGTCGTCCAGCCCCAGCCCC
TGGTGGTAGTAAAGGAGGAGAAGATTCACTCACCAATCATTCGCAGCGAGCCTTTCAGCACCTCACTTCG
ACCAGAGCCCCCCAAGCACCCAGAGAACATCAAGGCCCCAGTCCACCTGCCCCAGCGGCCTGAGATGAAG
CCTGTAGACATAGGGAGGCCTGTGATCCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGGCCCCTGACA
AGGACAAACAGAAGCAGGAGCCAAAAACACCAGTGGCACCCAAAAAGGACCTGAAAATTAAGAACATGGG
CTCCTGGGCCAGCCTGGTACAGAAGCATCCGACCACCCCATCCTCCACAGCCAAGTCCTCAAGTGACAGC
TTTGAGCATTTCCGCCGTGCTGCTCGGGAGAAGGAGGAGAGGGAAAAGGCCCTGAAGGCTCAGGCTGAGC
ATGCAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAGCCGAGAGGATGAAGATGCGCTGGA
ACAGGCACGTCGAGCCCATGAGGAGGCACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGAGA
CAGGAGCAGCAGCAGCAGCAGCAGCAGGCAGCTGCTGTGGCCGCTGCCTCTGCCCCCCAGGCCCAGAGCT
CCCAGCCCCAGTCTATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAACGAGAGCAGGAGCGGAGGCGCAG
GGAGGCAATGGCAGCTACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAAAATCTT
TTTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001286630
Insert Size 4206 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286630.1, NP_001273559.1
RefSeq Size 5995 bp
RefSeq ORF 4206 bp
Locus ID 57261
Gene Summary This gene was temporarily named bromodomain-containing 5 (Brd5) and was renamed bromodomain-containing 4 (Brd4). [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) encodes the longest isoform (3).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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