Myh14 (NM_001271540) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2400004E04Rik; II-C; NHMCII; NMHC II-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229692 representing NM_001271540 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGTGACCATGTCCGTGTCTGGGAGGAAGGTAGCCTCCAGGCCAGGCCCGGTGCCTGAGGCAG CCCAATCGTTCCTCTACGCGCCCCGGACGCCAAATGTAGGTGGCCCTGGAGGGCCACAGGTGGAGTGGAC AGCCCGGCGCATGGTGTGGGTGCCCTCGGAACTGCATGGGTTCGAGGCAGCAGCCCTGCGGGATGAAGGG GAGGAGGAGGCAGAAGTGGAGCTGGCGGAGAGTGGGCGCCGCCTGCGGCTGCCCAGGGACCAGATCCAGC GCATGAACCCACCCAAGTTCAGCAAGGCAGAAGATATGGCTGAGCTCACCTGCCTCAACGAGGCCTCGGT CCTGCACAACCTGCGAGAACGCTACTACTCCGGGCTCATTTATACCTACTCTGGCCTCTTCTGTGTGGTC ATTAACCCATACAAGCAGCTGCCCATCTACACGGAGGCCATTGTTGAAATGTACCGGGGCAAGAAGCGCC ATGAGGTGCCACCTCACGTGTATGCTGTGACGGAGGGCGCGTACCGCAGCATGCTTCAGGATCGTGAGGA TCAATCCATTCTCTGCACGGGAGAGTCTGGCGCTGGGAAGACGGAGAACACCAAGAAGGTCATCCAGTAC CTGGCCCATGTGGCATCATCTCCAAAGGGCAGGAAGGAGCCTGGTGTCCCTGCCTCCGTCAGCACCATGT CTTATGGGGAGCTAGAGCGTCAGCTTCTTCAAGCCAACCCCATCCTAGAAGCCTTTGGCAATGCCAAGAC GGTGAAGAACGACAACTCTTCCCGATTTGGCAAATTCATCCGCATCAACTTTGATATTGCTGGCTACATC GTGGGAGCAAACATCGAGACCTATCTGTTGGAGAAGTCCCGGGCCATCAGACAGGCCAAGGATGAATGCA GCTTCCATATCTTCTACCAGCTGCTAGGGGGCGCTGGGGAGCAGCTAAAAGCTGACCTCCTTCTGGAGCC CTGTTCCCATTATCGCTTCCTGACCAATGGGCCCTCATCGTCCCCGGGCCAGGAGCGTGAGTTATTCCAG GAGACCCTGGAGTCCCTGCGTGTGCTGGGCCTCCTCCCAGAAGAGATCACTGCCATGCTGCGCACTGTCT CTGCTGTCCTCCAGTTTGGCAACATTGTCCTGAAGAAAGAGCGCAATACGGACCAAGCCACCATGCCTGA CAACACAGCTGCCCAGAAGCTTTGCCGCCTCTTGGGACTCGGAGTGACCGACTTCTCCAGAGCCCTTCTC ACACCCCGCATCAAAGTGGGCCGAGATTATGTTCAGAAAGCACAAACCAAGGAGCAGGCTGACTTTGCGC TGGAGGCTCTGGCCAAAGCTACCTATGAGCGCCTGTTCCGCTGGCTGGTTCTGCGGCTCAACCGTGCCCT GGACAGAAGCCCGCGGCAGGGTGCCTCCTTCCTGGGCATCCTGGACATCGCGGGCTTTGAGATCTTCCAG CTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTACAGCAGCTATTCAACCACACCA TGTTCGTGCTGGAGCAGGAGGAGTACCAGCGAGAGGGCATCCCCTGGACCTTCCTAGACTTCGGGTTGGA CCTGCAACCTTGCATCGACCTCATTGAGCGTCCGGCCAACCCTCCAGGTCTCCTGGCCCTGCTGGACGAG GAGTGCTGGTTCCCCAAGGCCACGGACAAGTCTTTTGTGGAGAAGGTTGCCCAGGAGCAGGGCAGCCACC CCAAGTTCCAGCGCCCCAGGAACCTGCGAGATCAGGCCGACTTCAGCGTCCTGCACTATGCCGGCAAGGT TGACTACAAAGCCAGTGAGTGGCTGATGAAGAACATGGACCCACTGAATGACAATGTGGCCGCCTTGCTT CACCAGAGCACGGATCGTCTCACAGCTGAGATCTGGAAGGATGTGGAGGGCATCGTGGGGCTGGAGCAAG TAAGCAGCCTTGGAGATGGCCCACCGGGAGGCCGCCCCCGCCGTGGAATGTTCCGGACTGTGGGGCAGCT CTACAAAGAATCCCTGAGCCGCCTCATGGCCACGCTCAGCAACACCAACCCTAGTTTTGTCCGCTGCATC GTTCCCAATCATGAGAAGAGGGCTGGAAAGCTGGAGCCGCGCCTGGTGCTGGACCAACTCCGTTGTAACG GGGTCCTCGAGGGTATCCGCATCTGTCGCCAAGGCTTCCCCAACCGCATCCTCTTCCAGGAGTTCCGACA GCGCTATGAAATCCTCACCCCGAACGCTATTCCCAAGGGCTTCATGGACGGCAAACAGGCCTGTGAGAAG ATGATCCAGGCCCTGGAGCTAGACCCCAACCTGTACCGTGTTGGCCAAAGCAAGATCTTCTTCCGGGCAG GGGTCCTGGCCCAGCTGGAGGAGGAGCGGGACCTGAAAGTCACCGACATCATAGTGTCTTTCCAGGCAGC GGCACGGGGCTACCTGGCCCGTAGGGCTTTCCAGAGACGGCAGCAGCAGCAGAGTGCTCTGAGGGTGATG CAGAGAAACTGTGCTGCCTACCTCAAGCTCAGGAACTGGCAGTGGTGGAGGCTGTTCATCAAGGTGAAGC CCCTGCTGCAGGTGACACGGCAGGATGAGGTGCTGCAGGCGCGCGCCCAGGAGCTGCAGAAAGTTCAGGA GCTGCAGCAGCAGAGCGCTCGTGAAGTGGGGGAACTGCAGGGTCGAGTGGCACAGCTAGAGGAGGAGCGC ACGCGCCTGGCTGAGCAGCTTCGAGCAGAAGCCGAGCTCTGCTCTGAGGCCGAGGAGACGCGGGCGCGAC TGGCTGCCCGGAAGCAGGAGCTGGAGCTGGTGGTGACAGAGCTGGAGGCACGAGTGGGCGAGGAAGAAGA GTGCAGCCGGCAGCTGCAGAGTGAGAAGAAGAGGCTGCAGCAGCATATCCAGGAGCTAGAGAGCCACCTG GAAGCTGAGGAGGGTGCCCGGCAGAAGCTACAGCTGGAGAAGGTGACCACAGAGGCCAAGATGAAGAAAT TTGAGGAGGACCTGCTGCTCCTGGAGGACCAGAATTCCAAGCTGAGCAAGGAGCGGAGGCTGCTGGAGGA GCGGCTGGCTGAGTTCTCCTCACAGGCAGCAGAAGAGGAAGAGAAAGTCAAAAGTCTCAACAAGCTGAGG CTCAAATATGAAGCCACAATCTCAGACATGGAAGACCGGCTGAAGAAGGAGGAGAAGGGACGCCAGGAAC TAGAGAAGCTGAAGCGACGGCTGGACGGGGAGAGCTCAGAGCTTCAGGAGCAGATGGTGGAGCAGAAGCA GAGGGCAGAGGAACTGCTCGCACAGCTGGGCCGCAAGGAGGATGAGCTGCAGGCCGCCCTGCTCAGGGCA GAGGAAGAGGGTGGTGCCCGTGCCCAGTTGCTCAAGTCCCTGCGAGAGGCACAGGCTGGCCTTGCTGAGG CTCAGGAGGACCTGGAAGCTGAGCGGGTAGCCAGGGCCAAGGCGGAGAAGCAGCGCCGGGACCTGGGCGA GGAGTTGGAGGCCCTACGTGGGGAGCTCGAGGACACTCTGGATTCCACCAACGCCCAGCAGGAGCTGCGG TCCAAGAGGGAGCAGGAGGTGACAGAGCTGAAGAAAGCATTGGAAGAGGAGTCCCGTGCCCATGAGGTGT CCATGCAGGAGCTGAGACAGAGGCATAGCCAGGCACTGGTGGAGATGGCCGAGCAGTTGGAGCAAGCCCG GAGGGGCAAAGGTGTGTGGGAGAAGACTCGGCTATCCCTGGAGGCTGAGGTGTCCGAGCTGAAGGCCGAG CTGAGCAGCCTGCAGACCTCGAGACAGGAGGGTGAGCAGAAGAGGCGCCGCCTGGAGTCCCAGCTACAGG AGGTCCAGGGCCGATCCAGTGATTCGGAGCGGGCTCGGTCTGAGGCTGCTGAGAAGCTGCAGAGAGCCCA GGCGGAACTTGAGAGCGTGTCCACAGCCCTGAGTGAGGCGGAGTCCAAAGCCATCAGGCTGGGCAAGGAG CTGAGCAGTGCAGAGTCCCAGCTGCATGACACCCAGGAACTGCTTCAGGAGGAGACCAGGGCAAAGCTGG CCTTGGGGTCCCGTGTGCGTGCCCTAGAGGCCGAGGCGGCGGGGCTTCGGGAGCAGATGGAAGAGGAGGT GGTTGCCAGGGAACGGGCTGGCCGGGAGCTGCAGAGCACGCAGGCCCAGCTCTCTGAATGGCGGCGCCGC CAGGAAGAAGAGGCCGCGGTGCTGGAGGCTGGGGAGGAGGCTCGGCGCCGTGCAGCCCGGGAGGCAGAGA CCCTGACCCAGCGCCTGGCAGAAAAGACTGAGGCTGTAGAACGACTGGAGCGAGCCCGGCGCCGACTGCA GCAGGAGTTGGACGATGCCACTGTGGATCTGGGGCAGCAGAAGCAGCTCCTGAGCACACTGGAGAAGAAG CAGCGGAAATTTGACCAGCTCCTGGCAGAGGAGAAGGCTGCAGTTCTACGGGCTGTGGAAGACCGTGAAC GGATAGAGGCCGAAGGCCGGGAGCGAGAGGCCCGGGCCCTGTCGCTGACCCGGGCCCTGGAAGAGGAGCA GGAGGCCCGGGAGGAGCTGGAGAGGCAGAACCGTGCTCTGAGGGCTGAGCTGGAAGCACTGCTGAGCAGC AAGGATGACGTGGGCAAGAACGTGCACGAGCTGGAGCGAGCCCGTAAGGCGGCTGAACAGGCAGCCAGTG ACCTGCGGACACAGGTGACAGAATTGGAGGATGAGCTGACAGCCGCAGAGGATGCCAAGCTGCGCCTGGA GGTGACTGTGCAGGCTCTGAAGGCTCAACATGAACGCGACCTGCAGGGCCGCGATGATGCCGGTGAGGAG AGGCGGAGGCAGCTGGCCAAGCAGCTAAGAGACGCAGAGGTAGAGCGCGATGAGGAACGGAAGCAGAGGG CACTGGCTATGGCTGCCCGCAAGAAGCTGGAGCTGGAACTGGAGGAGTTGAAGGCGCAGACATCTGCTGC TGGGCAGGGCAAGGAAGAGGCAGTGAAGCAGCTGAAGAAGATGCAGGTCCAGATGAAGGAGCTGTGGCGG GAGGTAGAGGAGACGCGTAGCTCCCGCGACGAGATGTTTACCCTGAGCAGGGAAAATGAGAAGAAGCTCA AGGGGCTGGAAGCTGAGGTGCTGCGTCTGCAAGAGGAACTTGCTGCCTCAGACCGAGCCCGGAGGCAGGC CCAGCAAGACAGAGACGAGATGGCAGAGGAGGTGGCCAGTGGCAATCTTAGCAAGGCAGCCACCCTGGAG GAAAAACGGCAGCTGGAGGGGCGACTGAGCCAGTTGGAAGAGGAGCTGGAGGAAGAACAGAACAACTCGG AGCTGCTCAAGGACCATTACCGAAAGCTAGTGCTACAGGTCGAGTCCCTCACCACAGAACTGTCTGCCGA ACGAAGTTTCTCAGCCAAGGCCGAGAGTGGACGGCAGCAGCTGGAGCGGCAGATCCAGGAACTGCGGGCC CGCTTGGGTGAAGAGGATGCTGGAGCCCGAGCCAGGCAGAAAATGCTGATCGCTGCTCTGGAGTCTAAAC TGGCCCAGGCAGAGGAGCAGCTGGAGCAGGAGAGCAGGGAGCGCATCCTCTCTGGCAAGCTGGTACGCAG AGCTGAGAAGCGGCTGAAGGAGGTAGTTCTTCAGGTGGATGAAGAGCGCAGGGTGGCTGACCAGGTCCGG GACCAGCTGGAGAAAAGCAACCTCCGGCTGAAGCAGCTCAAGAGGCAGCTGGAGGAGGCAGAGGAGGAGG CATCTCGGGCACAGGCTGGTCGGAGGCGGCTGCAGCGGGAGCTGGAGGACGTCACTGAGTCTGCAGAATC CATGAACCGGGAGGTGACCACGCTGAGGAACAGGCTCCGGCGTGGCCCACTTACATTCACCACACGGACT GTGCGCCAGGTGTTCCGGCTGGAAGAGGGCGTGGCTTCTGACGAGGAAGAGGCTGAAGGAGCTGAACCTG GCTCTGCACCAGGCCAGGAGCCGGAGGCTCCGCCCCCTGCCACACCCCAATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271540
Insert Size	6003 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271540.1, NP_001258469.1
RefSeq Size	6457 bp
RefSeq ORF	6003 bp
Locus ID	71960
UniProt ID	Q6URW6
Gene Summary	Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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