Dst (NM_010081) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310001O04Rik; A830042E19Rik; ah; athetoid; AW554249; BP230; Bpag; Bpag1; BPAG1-n; dt; Macf2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229757 representing NM_010081 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGTAGTAGCTACAGTTACCGTAGCAGTGACTCTGTGTTTAGTAACACCACCAGCACTCGGACTA GTCTGGATTCCAATGAGAATCTCCTGTCCGTTCATTGTGGTCCCACGCTGATCAACTCCTGCATTAGCTT CAGTAATGAGTCCTTGGATGGACACAGGTTGGATATGCTGCAGCAGATTGCCACCCGAGTTCAGAGGGAT AGTGTCAGCTGCGAAGACAAACTCATCCTCGCCCGAAATGCTCTCCAGTCTGATTCCAAAAGATTAGAGT CGGGAGTCCAGTTTCAGAATGAAGCAGAGATTGCCGGGTACATACTTGAATGTGAGAACCTTTTACGCCA GCATGTGATTGATGTACAGATTCTGATAGATGGGAAATACTACCAGGCCGATCAGCTGGTGCAGAGGGTT GCAAAGCTGCGTGATGAGATTATGGCCTTAAGGAACGAATGTTCTTCGGTGTACAGCAAAGGACGCATGC TGACCACGGAACAGACCAAGCTCATGATATCAGGAATCACTCAAAGCTTAAACTCAGGATTTGCACAAAC CTTACACCCCAGCCTGAACTCAGGGCTAACCCAGAGTTTAACCCCTTCCTTGACGTCTTCTAGCGTGACT TCTGGCTTGTCGTCCGGGATGACTTCCCGCCTGACACCATCAGTTACTCCGGTGTATGCACCTGGGTTCC CTTCAGTTGTAGCTCCAAACTTCAGTTTAGGGGTAGAGCCAAATTCATTACAGACTCTGAAGCTAATGCA GATCCGAAAGCCCCTTCTCAAGTCGTCTTTGCTGGATCAGAACTTGACAGAAGAGGAAGTCAATATGAAA TTTGTTCAAGATCTCTTGAACTGGGTTGATGAGATGCAGGTGCAGCTGGACCGCACTGAATGGGGCTCAG ATTTGCCAAGTGTTGAAAGCCATTTAGAAAACCATAAAAATGTTCACCGAGCCATTGAAGAATTTGAATC CAGCCTTAAGGAAGCTAAAATTAGTGAGATTCAGATGACAGCGCCTCTGAAACTCTCTTACACAGATAAG TTGCACAGATTAGAGAGTCAGTACGCTAAACTCTTGAATACATCCAGAAATCAGGAGCGACATCTCGATA CACTCCACAACTTTGTAACCCGCGCAACTAACGAGCTTATCTGGTTGAATGAAAAGGAGGAGTCGGAAGT TGCTTATGATTGGAGTGAGAGAAACAGCAGTGTCGCCCGGAAGAAAAGCTACCACGCGGAGCTGATGCGG GAGCTGGAGCAGAAGGAGGAGAGCATTAAAGCTGTTCAGGAGATCGCAGAGCAGCTGCTTCTGGAAAACC ACCCAGCCCGCTTGACCATCGAGGCGTACAGAGCGGCCATGCAGACACAGTGGAGCTGGATCCTACAGCT GTGCCAGTGCGTGGAGCAGCACATTCAGGAGAACTCTGCCTATTTCGAGTTTTTCAATGATGCCAAAGAA GCCACTGATTACCTAAGGAATCTGAAAGATGCCATCCAGCGCAAGTACAGCTGTGACAGGTCCAGCAGCA TTCACAAACTGGAAGACCTTGTCCAGGAGTCCATGGAAAAGGAGGAGCTCCTGCAGTACCGGAGTGTGGT GGCAGGGCTGATGGGGCGAGCGAAGACAGTGGTGCAGCTGAAGCCGAGGAATCCTGACAACCCACTCAAG ACGTCCATCCCCATCAAAGCCATCTGCGACTACAGACAGATTGAGATAACCATTTACAAAGACGATGAGT GTGTCTTGGCAAACAACTCTCATCGAGCTAAATGGAAGGTCATCAGTCCTACTGGGAACGAGGCCATGGT CCCGTCTGTGTGCTTTACTGTTCCCCCACCAAACAAAGAAGCAGTTGACTTTGCCAACAGAATTGAGCAA CAGTATCAGAGTGTACTCACTCTCTGGCATGAGTCTCACATAAACATGAAGAGTGTCGTCTCCTGGCACT ATCTAGTCAATGAAATCGACAGAATTCGAGCTAGTAATGTGGCTTCGATAAAGACGATGTTGCCTGGCGA GCACCAGCAGGTTCTCAGTAATCTCCAGTCCCGCTTGGAGGATTTCCTGGAGGATAGCCAGGAATCCCAG ATATTCTCAGGCTCGGATATAAGTCAACTAGAAAAGGAAGTGAGTGTGTGCAGGAAGTATTATCAAGAGC TCCTCAAGTCTGCAGAAAGAGAGGAGCAAGAGGAATCTGTGTATAATCTTTACATCTCGGAAGTGCGAAA CATTCGGCTCCGCCTGGAGAGCTGTGAAGACAGGTTGATTCGGCAGATCCGGACTCCCCTGGAAAGAGAT GATTTACATGAAAGCATGCTCAGGATCACGGAGCAGGAGAAACTGAAGAAAGAGCTGGATCGGCTTAAAG ATGACTTGGGAACGATCACAAATAAGTGTGAAGAGTTTTTCAGCCAAGCGGCTGACTCGCCGTCTGTCCC CGCTCTCCGATCAGAGCTCAGCGTGGTCATTCAGAGCTTGAGTCAGATCTACTCTATGTCTTCCACGTAC ATAGAGAAATTGAAGACTGTGAATTTGGTTTTAAAAAACACTCAAGCTGCAGAAGCCCTTGTCAAACTCT ACGAAACCAAACTGTGTGAAGAGGAAGCAGTTATAGCAGACAAGAATAACATTGAGAATTTAATGAGTAC TTTAAAGCAATGGCGATCAGAAGTAGATGAGAAGAGAGAGGTGTTCCATGCATTGGAAGACGAGTTGCAG AAAGCAAAAGCCATCAGCGATGAGATGTTCAAGACACACAAAGAACGGGACCTTGACTTTGACTGGCACA AAGAAAAGGCAGACCAGCTTGTCGAAAGGTGGCAGAGCGTTCACGTCCAGATCGACAACAGGCTCCGGGA CTTAGAAGGAATCGGGAAGTCCCTGAAGCACTACAGGGACTCCTACCACCCTCTCGATGACTGGATCCAG CACATTGAAACGACCCAGAGAAAAATTCAGGAAAATCAGCCAGAAAACAGCAAAGCCCTGGCCCTGCAGC TGAACCAACAGAAGATGCTGGTGTCTGAAATAGAGGTGAAGCAGAGCAAAATGGACGAGTGTCAGAAATA CTCAGAGCAGTACTCGGCTGCCGTCAAGGACTATGAGTTGCAAACAATGACCTACAGGGCTATGGTGGAG TCCCAGCAGAAGTCGCCAGTGAAGCGACGGCGGATACAGAGCTCAGCAGACCTCGTTATTCAAGAGTTCA TGGACCTACGGACACGGTACACTGCCCTGGTCACTCTTATGACGCAGTATATTAAATTTGCTGGTGATTC GTTGAAAAGGCTGGAAGAGGAGGAGATGAAAAGGTCTAAAGAGAATTCTGAGCACGGGGCGTATTCAGAT CTACTGCAGCGTCAGAGAGCAACCATGGTTGAGAATAGCAAACTTACCGGGAAGATCAGTGAACTGGAAA CAATGGTGGCTGAGCTAAAGAAACAGAAATCCCGGGTCGAGGAGGAGCTTCCGAAAGTTAAGGAGGCCGC CGAAAATGAACTGAGGAAGCAACAGAGGAACGTGGAAGACATCGCCCTGCAGAAGCTGAGGGCCGAGAGC GAGGCCAAGCAGTACCGCCGTGAGCTGGAGACGATCGTGAGGGAGAAGGAAGCTGCCGAGCGGGAGCTGG AGCGGGTGAGGCAGCTCACGGCGGAGGCGGAAGCTCGGCGAGCCGCGGTGGAGGAGAACCTCCGCAACTT CCGGAGCCAGCTGCAGGAAAACACCTTCACCAGGCAGACGCTGGAAGATCATCTGCGGAGGAAAGACTCG AGCCTCAGTGATCTGGAACAGCAGAAACGGGCCCTGGTGGAAGAACTGCAACGAAAGCGGGACCACGAGG AGGAGCTCCTGCGACTGGTGAAGCAGATGGAGCGGGACCTGGCCTTCCAGAAGCAGGTGGCCGAGAAGCA GCTGAAGGAAAAGCAGAAGGTCGAGTTGGAAGCCAGAAGGAAAATCACCGAAATTCAGTTTTCATGTAGA GAGAGCGCTGCGGTGGCACAGGCACGGCCCCAGAGGGAGCAGGGCAGACAGAAAGAGGAGGAGCTGAAGC AGCAGGTGGATGAGCTGACCTTGGCCAATAGGAAGGCAGAGAAAGAGATGAGGGAGCTGAAGTATGAACT CAGCGCGGTCCAGCTCGAAAAGGCCTCGTCTGAGGAAAAGGCCCGGCTGCTGAAGGATAAATTGGACGAG ACGAATAACACACTCAAGTGCCTCAAGGAAGATCTGGAAAGGAAGGATCAGGCCCAGGAACGCTATTCCC AGCAGCTCAGAGACCTTGGCAGGCAGCTGAACCAAACAACAGACAAAGCTGAAGAGGTCAGGCAGGAAGC AAACGATCTCAAGAAAATAAAGCACACCTATCAGTTGGAATTAGAGTCTCTCCATCAAGAGAAAGGAAAA CTCCAGAGGGAAGTTGACAGAGTCACCAGGGCGCACGCACTAGCCGAGAGGAACATTCAGTGTTTAAACT CCCAAGTTCACGCTTCGAGGGATGAGAAGGACCTCTCAGAAGAGAGGAGACGGCTCTGCCAGAGAAAATC AGACCATTTAAAAGAAGAGTTTGAGAGGAGCCATGCACAGTTGCTTCAGAATATTCAAGCCGAGAAAGAA AATAATGATAAAATCCAAAAGCTGAATAAAGAGCTGGAGAAAAGCAATGAGTGCGCAGAAACGCTCAAGC AGAAGGTGGATGAACTCACTAGGCAGAATAATGAAACCAAATTGATGATGCAGAGGATTCAGGCAGAGTC CAAGAACATAGTCCGCGAGAAGCAAGCCATCCAGCAGAGATGCGAAGTGCTGCGGATCCAGGCAGACGGT TTTAAGGATCAGTTACGGAACACCAACGAACACTTGCATAAGCAGACAAAAACAGAGCAGGATTTCCACA GAAAAATTAAAAGCCTCGAAGATGATCTAGCCCAAAGTCAAAACCTAGTAAGCGAGTTCAAGCAAAAGTG TGACCAGCAGAGCATGATCATCCAGAAAACGGAGAAGGAGGTGAGGAGCCTGAGCGCAGAACTGAGCGCG TCCAAAGAGGAGAAGCGGCGGGAAGAGCAGAAGGCTCAGCTGCAGCGAGCTCAGGTGCAGGAGCTGAATG ACAGGCTGAAGAGGGTGCAAGACGAGCTGCACCTGAAGACCATCGAAGAGCAGATGACCCACAGGAAGAT GATCCTGCTCCAGGAAGAGTCGGATAAGTTCAAACGCTCGGCAGATGAGTTTCGGAAGAAGATGGAGAAG CTAATGGAGTCCAAGGTCGTCACTGAAACCGATCTCTCAGGCATCAAGCACGACTTTGTGTCTCTCCAGA GAGAAAACTTCCGGGCGCAAGAGAACGCGAAGCTGTGGGAGACCAACATTCGAGAGCTTGAGCGGCAGCT TCAGTGCTACCGTGAGAAGATGCAGCAAGGCCCGCCCGTGGAAGCAAACCACTATCAGAAGTGCCGGAGA CTCGAGGAGGAGCTGTTGGCCCAGAGACGGGAAGTTGAAAACCTCAAACAAAAAATGGACCAGCAGATAA AGGAACATGAGCACCAGTTGCTTCGGCTCCAGTGTGAAATCCAAAAGAAGAGCACAACCCAAGACCACAC CTTCGCGTCGGCTTTTGATACGGCAGGGAGAGAGTGCCACCACCCTGCAGAGATCTCCCCTGGGAACTCT GGGCACCTTAACCTAAAGACCAGACTCCCACTGTCCAGGTGGACTCAGGAGCCACACCAGACAGAAGGAA AATGGCCGCACAGGGCTGCTGAACAACTTCCGAAGGAGGTTCAGTTCCGACAGCCAGGGGCTCCGCTGGA CAGGGAGAGCAGCCAGCCATGCTACTCGGAGTATTTTTCTCAGACAAGCACTGAACTGCAGATAACTTTT GATGATAAAAACCCAATCACGCGACTGTCTGAACTAGAGACGATGAGGGAGCAAGCCCTGCATCCCTCCA GACCGCCGGTGACGTATCAGGATGACAAACTTGAAAGGGAGTTGGTGAAGCTTTTGACACCCTTAGAGAT AGCTAAGAACAAACAGTGTGGCATGCATACAGAAGTCACGACCTTAAAACAAGAGAAGAGGCTGGGTTCC AGTGCTGGTGGATGGATGCTGGAAGGGTGCAGAACATCTGGTGGACTCAAGGGAGATTTCCTTAAGAAAA GCGTAGAGCCAGAGGCTTCCCCGAGCCTTGACCTTAACCAGGCGTGCTCTGTTAGGGATGAGGAGTTTCA GTTTCAAGGGCTCAGGCACACCGTGACTGGCAGGCAGCTGGTCGAAGCCAAGCTCCTGGACATGAGGACA GTTGAGCAGCTGCGGCTTGGTCTGAAGACTGTCGAAGAAGTTCAGAGAAGTCTTAGCAAGTTTCTGACCA AAGCTACCTCCATTGCAGGACTTTATCTAGAATCTTCCAAAGAAAAAATGTCGTTCACTTCGGCGGCCCA GAAAATCATAATAGACAAAATGATAGCTCTAGCCTTTTTAGAAGCTCAGGCTGCAACAGGTTTTATAATT GATCCGGTTTCTGGTCAGACTTACTGTGTTGAAGATGCAGTTCTTCATGGCATCGTTGATCCTGAGTTCA GGAGCAGGCTCCTGGAGGCAGAGAAGGCAGTTTTGGGATATTCACATGCTTCTAAGACGCTGTCAGTGTT CCAGGCAATGGAAAATAGAATGCTTGATAGGAAGAAAGGTAAACATATCTTGGAGGCACAAATTGCCAGT GGGGGCGTCATTGACCCTGTGAGAGGCGTCCGTGTCCCTCCAGAAATGGCTGTGCAGCAGGGCTTGCTGA ACAACGCCGTCCTACAGTTCCTGCATGAACCGTCCAGCAACACGAGAGTCTTTCCTAATCCCAACAACAA GCAGGCTCTGTATTATTCGGAGTTACTGCAGATCTGTGTGTTTGATGTAGATTGCCAGTGTTTTCTCTTG CCGTTTGGGGAGAGGGAAATATCCAATCTCAACATAGAGAAAACTCATAAAATTGCCGTGGTAGATACCA AAACTGGGGCGGAACTGACGGCATTCGAGGCTTTCCAGAGAAACCTGATTGACAAGGGTATTTATCTCGA ACTCTCAGGGCAGCAGTATCAGTGGAAGGAAGCTACATTTTTTGACTCCTACGGGCATCCTTCTCACATG CTGACTGATACTAAAACTGGTCTGCAGTTCAATATTAGTGAAGCTGTCGAGCAGGGAACGCTAGACAAAG CCTTGGTGCAAAAGTATCAGGAAGGCCTAACTACACTAACAGAACTGGCTGACTTTCTTCTGAGCAAGGT AGTTCCCAAGAAGGATTTGCACAGTCCCATTGCAGGCTATTGGCTGACCGCTAGTGGGGAGCGAATCTCC TTGCTGAAAGCCTCCCGTAGAAACTTGGTTGATAGGGTTACAGCCCTCAGATGCCTTGAAGCCCAAATCT GTACAGGAGGGATCATCGATCCCCTAACTGGGAAAAAGTACAGGGTGGCCGAGGCTTTGCATAGAGGGCT GGTGGACGAGGGCTTCGCCCAGCAGCTACGCCAGTGTGAATTAGTGATCACGGGGATCAGCCACCCCGTC AGCAATAAAATGATGTCAGTGGTGGAAGCCGTGAATGCAAATATCATCAGTAAAGAAATGGGCATGCGGT GCCTGGAATTTCAGTACCTGACAGGGGGCCTGATAGAGCCGAAGGTTTTCTCGAGGCTGACCATAGAAGA GGCCCTTCACGTCGGTATCATTGATGTCCTCATCGCCACCAGACTCAAAGATCAAAAGTCATATGTCAGG GATATAATGTGCCCCCAGACTAAGAGAAAATTGACATATAAAGAGGCCCTGGAGAAAGCTGATTTTGATT TCCACACAGGACTTAAGCTGTTAGAAGTGTCTGAACCCTTGGGGACAGGGATATCCAACCTCTACTATTC TTCCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010081
Insert Size	7920 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010081.2, NP_034211.2
RefSeq Size	8788 bp
RefSeq ORF	7920 bp
Locus ID	13518
UniProt ID	Q91ZU6
Gene Summary	Cytoskeletal linker protein. Acts as an integrator of intermediate filaments, actin and microtubule cytoskeleton networks. Required for anchoring either intermediate filaments to the actin cytoskeleton in neural and muscle cells or keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. The proteins may self-aggregate to form filaments or a two-dimensional mesh. Regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport. Mediates docking of the dynein/dynactin motor complex to vesicle cargos for retrograde axonal transport through its interaction with TMEM108 and DCTN1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4, also known as e) has multiple differences in the coding region and UTRs compared to variant 1. This variant encodes isoform 4 (also known as e), which has distinct N- and C-termini and is significantly shorter than isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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