Ube2q1 (BC082275) Mouse Tagged ORF Clone

CAT#: MG203239

  • TrueORF®

Ube2q1 (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2Q (putative) 1 (cDNA clone MGC:90946 IMAGE:6310111)

ORF Plasmid: DDK tGFP



  "BC082275" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms NICE-5, PRO3094, Ube2q
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203239 representing BC082275
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGATCAGCCCTTGCCAGCAGAGCAGTGCACGCAGGAAGAAGTGTCTTCAGAAGATGAAGATGAAG
AGATGCCTGAGGATACAGAAGACCTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCCGAGGGCAAGAA
GTCTGAAGATGATGGCATCGGAAAAGAGAACCTGGCCATCCTAGAGAAAATTAAAAAGAACCAGAGGCAA
GATTACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATA
TATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTCTCTATGACTG
GAATGTCAAACTCCTCAAAGTTGACCAGGATAGCGCTTTGCACAATGATCTTCAGATCCTGAAGGAGAAG
GAAGGAGCAGACTTCATCCTACTTAACTTCTCCTTTAAAGATAACTTCCCCTTTGACCCACCGTTCGTCA
GGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGTGGAGGTGCCATCTGCATGGAACTTCTCAC
CAAGCAGGGCTGGAGCAGTGCCTACTCCATAGAGTCCGTGATCATGCAGATCAGCGCCACGCTGGTGAAG
GGGAAAGCACGGGTGCAGTTTGGAGCCAACAAATCTCAGTATAGCCTGACGAGAGCACAGCAGTCCTACA
AGTCCTTGGTGCAGATCCATGAAAAAAACGGCTGGTACACACCCCCAAAGGAAGATGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203239 representing BC082275
Red=Cloning site Green=Tags(s)

MLDQPLPAEQCTQEEVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKSEDDGIGKENLAILEKIKKNQRQ
DYLNGAVSGSVQATDRLMKELRDIYRSQSFKGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEK
EGADFILLNFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIESVIMQISATLVK
GKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPKEDG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC082275
ORF Size 761 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC082275, AAH82275
RefSeq Size 1686 bp
RefSeq ORF 761 bp
Locus ID 70093
Gene Summary Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Involved in female fertility and embryo implantation (PubMed:23108111). May be involved in hormonal homeostasis in females (PubMed:23108111). Involved in regulation of B4GALT1 cell surface expression, B4GALT1-mediated cell adhesion to laminin and embryoid body formation (PubMed:18511602).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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