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Cep57 (BC050785) Mouse Tagged ORF Clone
CAT＃: MG205263
- TrueORF®
Cep57 (tGFP-tagged) - Mouse centrosomal protein 57 (cDNA clone MGC:58550 IMAGE:6703205)
ORF Plasmid: DDK
"BC050785" in other vectors (4)
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CNY 3,900.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Tsp57, mKIAA0092 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205263 representing BC050785
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAAAGCATGCAGAAATGGAGAGGACATCTGTGTTAGAAAAGCAGGTTTCTCTAGAAAGAGAACGGC AGCATGATCAAACCCATGTTCAGAGCCAGCTGGAAAAACTGGATCTTCTTGAACAGGAATACAACAAACT CACCGCCATGCAGGCCCTTGCAGAAAAAAAGATGCAGGAGTTGGAGTCCAAACTCCGTGAAGAAGAGCAG GAAAGGAAGCGGATGCAGGCCCGGGCAGCAGAGTTACAGAGTGGTCTAGAAGCAAATAGACTTATTTTTG AAGATAAGACTACTTCATGTGTTTCAACCAGCACTAGAAAAATAAAAAAAAAGAAGTCAAAACCACCAGA AAAGAAAGGTTCTAGGACCTATTTTGGTGCACAGCCACATTACAGATTGTGCTTAGGTGATATGCCTTTT GTTGCTGGAACGTCCACCAGTCCCAGCCATGCCGTGGTAGCCAATGTTCAGCATGTCTTGCATCTGATGA AACACCACAGTAAAGCCCTGTGCAATGATCGAGTTGTTAACAGTGTTCCCTTGGCAAAGCAAGCTTGCTC ACGAGTTAGTAAGAGTAAGAAGTCAGTGGTGCCTCCTTCCAGCAGTGTTAATGAAGAACTTTCGGATGTC TTGCAGACTTTACAGGATGAATTTGGGCAAATGAGCTTTGATCATCAGCAGCTTACTAAACTCATACAGG AATCACCAACTGTGGAACTAAAAGACAACCTGGAGTGTGAATTGGAGGCATTAGTGGGAAGAATGGAAGC GAAGGCCAATCAGATAACTAAAGTTCGAAAATACCAAGCCCAGCTGGAGAAACAAAACATAGATAAGCAG AAGAAGGAATTAAAAGCTAACAAAAAGACTCTTGATGAAGAAGGGAACAGCAGCGGTCGTTCATCTGGAG TCCCAAGGACCGCAAGTAAGAAGGATCTGGCCAAACAGAGACCTGGAGAAAAAAGCAGGAAAAATCTTCA GTTACTGAAGGACATGCAGACCATACAGAATTCACTGCAGAGCAGTAATCTGTGTTGGGATTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205263 representing BC050785
Red=Cloning site Green=Tags(s) MIKHAEMERTSVLEKQVSLERERQHDQTHVQSQLEKLDLLEQEYNKLTAMQALAEKKMQELESKLREEEQ ERKRMQARAAELQSGLEANRLIFEDKTTSCVSTSTRKIKKKKSKPPEKKGSRTYFGAQPHYRLCLGDMPF VAGTSTSPSHAVVANVQHVLHLMKHHSKALCNDRVVNSVPLAKQACSRVSKSKKSVVPPSSSVNEELSDV LQTLQDEFGQMSFDHQQLTKLIQESPTVELKDNLECELEALVGRMEAKANQITKVRKYQAQLEKQNIDKQ KKELKANKKTLDEEGNSSGRSSGVPRTASKKDLAKQRPGEKSRKNLQLLKDMQTIQNSLQSSNLCWDY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC050785 |
| ORF Size | 1044 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC050785.1 |
| RefSeq Size | 1676 bp |
| RefSeq ORF | 1046 bp |
| Locus ID | 74360 |
| Gene Summary | Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218472 | Cep57 (untagged) - Mouse centrosomal protein 57 (cDNA clone MGC:58550 IMAGE:6703205), (10ug) |
CNY 6,940.00 |
|
| MR205263 | Cep57 (Myc-DDK-tagged) - Mouse centrosomal protein 57 (cDNA clone MGC:58550 IMAGE:6703205) |
CNY 3,520.00 |
|
| MR205263L3 | Lenti ORF clone of Cep57 (Myc-DDK-tagged) - Mouse centrosomal protein 57 (cDNA clone MGC:58550 IMAGE:6703205) |
CNY 5,420.00 |
|
| MR205263L4 | Lenti ORF clone of Cep57 (mGFP-tagged) - Mouse centrosomal protein 57 (cDNA clone MGC:58550 IMAGE:6703205) |
CNY 5,420.00 |
