Sdccag8 (BC016244) Mouse Tagged ORF Clone
CAT#: MG205648
- TrueORF®
Sdccag8 (tGFP-tagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575)
ORF Plasmid: DDK
"BC016244" in other vectors (4)
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CNY 4,090.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2700048G21Rik; 5730470G24Rik; Cccap |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205648 representing BC016244
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGTCCCCAGGGAACTCTACCCTGGAGGACAGTCTGGGGCAATACCAACGGAGTCTCCGGGAAC GTGCCAATAGAAGCATTCACCAGTTGAAATGTGCCCTGAGAGAAGTGGATGTCACTGTAGAAGAAGATGC ACTGGATCCTTCCACCAGCATCAACGTAGAAAATGAGGACACAGGGGTGGCCTGGCATGAGCTGCAGCAC AGCCATGCTGTCAATCAACTGAAAGCTTTGTTGCGTCAACAAACAAATAAGGAAAATGAGACATCTCCAC CGCGAAGACGAAAGTTGTCCCCTTCGCGGCCGTCAGAATGTGACGATGGCAGCATGCCCACCATGCACAA CCTCGTTCCTATCATTAACGACCAGTCTCAATATATCCACCACTTAGAAGCAGAAGTCAAGTTCTGCAAG GATGAATTGTCTGGAATGAAAAATAGGGTGCAAGTAGTTGTACTTGAGAATGAAAGGCTCCAGCAAGAAC TGAAATCTCAAAGACCGGAGGAGACATTGAGGGAGCAAACATTCCTGGATGCATCTGGAAACATGCAGAA TTCTTGGATTATGACACGAGAAGATTCTAGGGTGGATGAAGCTGCAAAGAGACCATTTTCTCATGGTGAT GCTGAAACTGGCAAAACTGCATCCACTGGTGATGCCAATAAATGGAAGCTGGAGCTGGAGAGGCTGAAAC TCACATACGAGGCGAAGACTGACCTTCTGGAATCTCAGCTGATGCTTCTGAGGAAAGACTTAGCTGAATA CCAGAAAACCTGTGAGGATCTTAAGGAAAGATTGAAGCATAAAGAGTCTCTTCTGGCTGCGAGTGCATCC AGCCGTGTGGGTGGCCTCTGTTTGAAATGTGCTCAGCATGAAGCTGTTCTTTCCCAGACACACAGCAACG TGCACATCCAGACCATCGAGAGGCTGACTAAAGAGAGAGATGACTTAATGTCTGTGCTAGTCTCTGTGCG GAGCAGCTTGGCAGAAGCGCAGAAGAGAGAGACAAGTGCCTATGAGCAGGTGAAGCATGCTGTGCAGATG ACAGAGGAAGCCAACTTTGAGAAGACCAAGACTGTATCCCTTGCGTGTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205648 representing BC016244
Red=Cloning site Green=Tags(s) MAKSPGNSTLEDSLGQYQRSLRERANRSIHQLKCALREVDVTVEEDALDPSTSINVENEDTGVAWHELQH SHAVNQLKALLRQQTNKENETSPPRRRKLSPSRPSECDDGSMPTMHNLVPIINDQSQYIHHLEAEVKFCK DELSGMKNRVQVVVLENERLQQELKSQRPEETLREQTFLDASGNMQNSWIMTREDSRVDEAAKRPFSHGD AETGKTASTGDANKWKLELERLKLTYEAKTDLLESQLMLLRKDLAEYQKTCEDLKERLKHKESLLAASAS SRVGGLCLKCAQHEAVLSQTHSNVHIQTIERLTKERDDLMSVLVSVRSSLAEAQKRETSAYEQVKHAVQM TEEANFEKTKTVSLACV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC016244 |
ORF Size | 1101 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC016244.1 |
RefSeq Size | 2042 bp |
RefSeq ORF | 1103 bp |
Locus ID | 76816 |
Gene Summary | Plays a role in the establishment of cell polarity and epithelial lumen formation (PubMed:20835237). Plays also an essential role in ciliogenesis and subsequent Hedgehog signaling pathway that requires the presence of intact primary cilia for pathway activation. Mechanistically, interacts with and mediates RABEP2 centrosomal localization which is critical for ciliogenesis (PubMed:27224062).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218488 | Sdccag8 (untagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575), (10ug) |
CNY 6,940.00 |
|
MR205648 | Sdccag8 (Myc-DDK-tagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575) |
CNY 3,710.00 |
|
MR205648L3 | Lenti ORF clone of Sdccag8 (Myc-DDK-tagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575) |
CNY 5,610.00 |
|
MR205648L4 | Lenti ORF clone of Sdccag8 (mGFP-tagged) - Mouse serologically defined colon cancer antigen 8 (cDNA clone MGC:28854 IMAGE:4507575) |
CNY 5,610.00 |
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