Syne4 (NM_153577) Mouse Tagged ORF Clone
CAT#: MG206061
- TrueORF®
Syne4 (tGFP-tagged) - Mouse expressed sequence AI428936 (AI428936)
ORF Plasmid: DDK
"NM_153577" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 0610012K07Rik; AI428936; KASH4; Nesp4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206061 representing NM_153577
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGTTCCACCTCTTGGCCGTGAATTCCCGCCAGAGCCTGTGAATTGTCCCCTGGCAGCTCCTA GAGAGCTGGATGTTGTCGGAGGGACCATCTGCCCTGCACCTGAGGAAGAGACAAGCAGGCCAGAGCAGGT CCAGGCCTCCTTGGGCCTGCCTGAGCATTGCATGGGTGAACTGAAGAGCACTGAGTCTGCCACCAGCCCC TCAAGACTCCCCCTGGCCTCTTCCCATGAGCATCAAGACGGGGGCAAGCCCTGTGAGCACTCTGACTCTG GTTTGGAAGTACTAGAAGCTGAACAGGACAGTCTACATCTTTGTCTGTTGAGGTTGAACTTCCGGCTGCA GGACCTGGAACGAGGCCTTGGGTCTTGGACGCTGGCCCACAACAGGATTGTCCAGATGCAGGCCCTGCAG GCAGAGCTACGAGGGGCCGCTGAGCGCGTGGATGCATTGCTTGCATTTGGTGAGGGCCTGGCAGAGAGGA GTGAGCCCAGGGCCTGGGCATCCCTGGAGCAGGTCCTGAGGGCCCTTGGAACCCACCGAGACACCATCTT CCAACGGCTCTGGCAGCTGCAGGCCCAGTTGATCAGCTATAGCCTGGTGCTTGAGAAGGCCAACCTCCTG GACCAGGACTTGGAAGTTGAGGGAGACTCAGACGGGCCAGCAGCTGGTGGAGTCTGGGGGCCCTGGGCAC CCAGTACCTTCCCCACTCCTGCAGAGTTGGAGTGGGACCCAGCAGGGGATGTTGGGGGTCTTGGGCCCTC AGGGCAAAAGATATCTCGGATACCAGGAGCTCCCTGTGAGCTGTGTGGCTACAGGGGACCCCAGAGCAGT GGACAGGGCCTTGAGGACCTGCTCTCCTTGGGGCTTGGCCACCGGAAACATTTAGCGGCTCACCATCGAA GGCGGCTCCGGAAGCCTCAGGACAGGAAGAGGCAAGTATCCCCCAGTCTGCCTGATGCAATGCTGGAAGT GGATCGCGGGGTCCCAGCTCCTGCATCCAAGCGGCCCCTGACCCTCTTCTTTCTCCTTCTCTTCCTCCTT CTGGTGGGTGCCACGCTGTTGCTGCCCTTGTCGGGGGTCTCCTGCTGTTCTCATGCCCGGCTGGCTAGGA CGCCCTACCTGGTGCTCAGTTATGTCAATGGTCTCCCTCCAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206061 representing NM_153577
Red=Cloning site Green=Tags(s) MALVPPLGREFPPEPVNCPLAAPRELDVVGGTICPAPEEETSRPEQVQASLGLPEHCMGELKSTESATSP SRLPLASSHEHQDGGKPCEHSDSGLEVLEAEQDSLHLCLLRLNFRLQDLERGLGSWTLAHNRIVQMQALQ AELRGAAERVDALLAFGEGLAERSEPRAWASLEQVLRALGTHRDTIFQRLWQLQAQLISYSLVLEKANLL DQDLEVEGDSDGPAAGGVWGPWAPSTFPTPAELEWDPAGDVGGLGPSGQKISRIPGAPCELCGYRGPQSS GQGLEDLLSLGLGHRKHLAAHHRRRLRKPQDRKRQVSPSLPDAMLEVDRGVPAPASKRPLTLFFLLLFLL LVGATLLLPLSGVSCCSHARLARTPYLVLSYVNGLPPI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153577 |
ORF Size | 1164 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153577.2, NP_705805.1 |
RefSeq Size | 1354 bp |
RefSeq ORF | 1167 bp |
Locus ID | 233066 |
UniProt ID | Q8CII8 |
Gene Summary | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning (By similarity). Behaves as a kinesin cargo, providing a functional binding site for kinesin-1 at the nuclear envelope. Hence may contribute to the establishment of secretory epithelial morphology, by promoting kinesin-dependent apical migration of the centrosome and Golgi apparatus and basal localization of the nucleus.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201741 | Syne4 (untagged) - Mouse expressed sequence AI428936 (AI428936), (10ug) |
CNY 3,656.00 |
|
MR206061 | Syne4 (Myc-DDK-tagged) - Mouse expressed sequence AI428936 (AI428936) |
CNY 3,656.00 |
|
MR206061L3 | Lenti ORF clone of Syne4 (Myc-DDK-tagged) - Mouse expressed sequence AI428936 (AI428936) |
CNY 5,230.00 |
|
MR206061L4 | Lenti ORF clone of Syne4 (mGFP-tagged) - Mouse expressed sequence AI428936 (AI428936) |
CNY 5,230.00 |