Gorasp1 (BC012251) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	P65, GOLPH5, GRASP65
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207123 representing BC012251 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTAGGGGCAAGCAGCGAGCAGCCGGCGGGCGGCGAGGGCTTCCATCTGCACGGGGTACAAGAGA ACTCGCCGGCCCAGCAGGCAGGCCTGGAGCCCTACTTCGACTTCATCATCACCATCGGGCACTCGAGGCT GAACAAGGAGAACGACACGCTGAAGGCATTGCTGAAGGCCAATGTGGAGAAGCCGGTGAAGCTGGAGGTA TTCAACATGAAGACCATGAAGGTGCGCGAGGTAGAGGTGGTGCCCAGCAACATGTGGGGCGGCCAGGGCC TCCTGGGAGCCAGCGTGCGCTTCTGTAGCTTCCGCAGGGCCAGCGAACACGTGTGGCATGTGCTGGATGT GGAGCCCTCTTCACCTGCTGCCTTGGCCGGCCTGTGCCCTTACACAGACTACATAGTTGGCTCTGACCAG ATTCTCCAGGAGTCAGAAGACTTCTTTACTCTCATTGAGTCCCATGAGGGGAAGCCTCTGAAGCTGATGG TGTATAACTCGGAGTCCGACTCCTGCCGGGAGGTGACTGTGACTCCCAATGCAGCCTGGGGTGGAGAGGG CAGTCTGGGGTGTGGTATTGGTTATGGGTACCTGCATCGGATCCCAACGCAACCCTCCAGCCAGCACAAG AAGCCACCCGGTGCCACACCACCTGGCACTCCAGCTACGACCTCACAACTTACTGCCTTTCCTCTTGGTG CCCCACCACCTTGGCCTATCCCTCAGGACTCTTCTGGCCCAGAGTTGGGTTCCAGGCAGAGTGACTTCAT GGAGGCCCTACCACAAGTCCCTGGTAGCTTTATGGAGGGACAGCTCCTTGGGCCTGGGAGTCCCAGCCAT GGCGCTGCTGACTGTGGGGGATGCCTGCGTGCTATGGAGATCCCGCTTCAGCCTCCACCTCCAGTGCAGC GGGTCATGGACCCAGGCTTCCTGGATGTATCAGGCATGTCCCTCCTGGACAGCAGCAACATAAGCGTGTG CCCCAGCCTGTCATCTTCCACAGTGCTGACCTCCACAGCTGTTTCAGTCTCAGGACCAGAGGACATTGGT TCTAGCAGCAGTTCTCACGAGCGGGGTGGTGAAGCCACGTGGTCAGGGTCAGAGTTTGAGATCTCCTTCC CAGACAGTCCAGGTGCCCAGGCCCAGGCGGACCACCTGCCCCGGCTGACTCTCCCCGATGGCCTCACATC TGCGGCCTCACCTGAAGAAGGGCTGTCTGCAGAGCTGCTGGAAGCACAGACTGAGGAGCCGGCAGACACA GCCAGCCTGGATTGCAGGGCAGAGACTGAGGGGAGAGCCAGCCAAGCGCAGGCCACCCCAGATCCAGAGC CTGGGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207123 representing BC012251 Red=Cloning site Green=Tags(s) MGLGASSEQPAGGEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEV FNMKTMKVREVEVVPSNMWGGQGLLGASVRFCSFRRASEHVWHVLDVEPSSPAALAGLCPYTDYIVGSDQ ILQESEDFFTLIESHEGKPLKLMVYNSESDSCREVTVTPNAAWGGEGSLGCGIGYGYLHRIPTQPSSQHK KPPGATPPGTPATTSQLTAFPLGAPPPWPIPQDSSGPELGSRQSDFMEALPQVPGSFMEGQLLGPGSPSH GAADCGGCLRAMEIPLQPPPPVQRVMDPGFLDVSGMSLLDSSNISVCPSLSSSTVLTSTAVSVSGPEDIG SSSSSHERGGEATWSGSEFEISFPDSPGAQAQADHLPRLTLPDGLTSAASPEEGLSAELLEAQTEEPADT ASLDCRAETEGRASQAQATPDPEPGL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC012251
ORF Size	1340 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC012251, AAH12251
RefSeq Size	3495 bp
RefSeq ORF	1340 bp
Locus ID	74498
Gene Summary	Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]

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