Chst2 (BC051963) Mouse Tagged ORF Clone

CAT#: MG207740

  • TrueORF®

Chst2 (tGFP-tagged) - Mouse carbohydrate sulfotransferase 2 (cDNA clone MGC:62233 IMAGE:5707543)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,130.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Chst2 Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Chts2, GlcNAc6ST, Gn6st, GST-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207740 representing BC051963
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTATTTCGCAGGAAGGCGCTGGTGCTGTGCGCGGGCTATGCACTGCTACTGGTGCTCACGATGC
TCAACCTCTTGGACTACAAGTGGCATAAAGAGCCGCTGCAGCAGTGCAACCCCGACGGGCCTCTGGGTGC
CGCGGTAGGGGCGGCCGGGGCCGGCTGGGGACGGCCGGGGTCGCCTCCTGCAGCGCCACCCCGCGCTCAC
TCTCGCATGGACCCCCGCACCCCGTACCGCCCTCCTGCCGCGGGCGTGGGGGCAGTTCCCGCAGCCGCGG
CTGGGAGTGCAGGAGCTGCGGCCTCTCTGGGCAATGCTACTCGAGGCACCAGGGGTGGAGGGGACAAGCG
GCAGTTGGTGTATGTGTTCACCACGTGGCGCTCGGGCTCGTCCTTCTTCGGTGAGCTCTTCAACCAGAAC
CCTGAGGTGTTCTTCCTCTATGAGCCTGTGTGGCACGTGTGGCAAAAACTGTACCCCGGGGACGCCGTTT
CCCTGCAGGGGGCAGCGCGGGACATGCTGAGCGCTCTCTACCGCTGCGATCTTTCGGTTTTCCAGCTGTA
TAGCCCCGCAGGCAGTGGGGGGCGCAACCTCACCACTCTGGGCATCTTTGGGGCAGCCACTAACAAGGTG
GTATGCTCCTCGCCACTCTGTCCTGCCTACCGCAAGGAGGTCGTCGGACTGGTGGACGACCGCGTGTGCA
AAAAGTGCCCACCTCAACGCCTGGCACGCTTCGAGGAGGAGTGTCGCAAGTACCGCACGCTGGTTATCAA
GGGCGTGCGGGTCTTCGATGTGGCTGTGTTGGCGCCGCTGCTTAAAGATCCAGCCTTGGACCTCAAGGTC
ATCCACCTAGTACGTGATCCTCGTGCTGTTGCCAGCTCCCGCATCCGCTCGCGTCACGGCCTCATCCGGG
AAAGCCTACAGGTGGTGCGAAGCCGGGATCCAAGAGCCCACCGCATGCCCTTCCTGGAGGCTGCTGGCCA
CAAGCTTGGTGCCAAGAAGGAGGGTATGGGTGGCCCAGCAGACTACCACGCTCTGGGTGCAATGGAGGTC
ATCTGCAACAGTATGGCCAAGACGCTGCAAACAGCCCTGCAGCCTCCTGACTGGCTGCAGGGACACTACT
TGGTGGTGAGGTACGAGGATCTGGTGGGAGACCCCGTTAAGACCCTACGGAGGGTATATGACTTTGTGGG
GCTGCTGGTGAGTCCCGAAATGGAGCAGTTTGCCCTGAACATGACCAGTGGTTCGGGCTCCTCCTCCAAG
CCTTTCGTGGTGTCAGCTCGCAATGCCACTCAGGCCGCCAATGCCTGGCGGACCGCGCTCACCTTCCAGC
AGATCAAACAGGTGGAGGAGTTTTGCTACCAGCCCATGGCCGTGCTGGGCTATGAGCGGGTTAACAGTCC
TGAGGAGGTCAAAGACCTCAGCAAGACCTTGCTCAGGAAGCCCCGGCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207740 representing BC051963
Red=Cloning site Green=Tags(s)

MKVFRRKALVLCAGYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAVGAAGAGWGRPGSPPAAPPRAH
SRMDPRTPYRPPAAGVGAVPAAAAGSAGAAASLGNATRGTRGGGDKRQLVYVFTTWRSGSSFFGELFNQN
PEVFFLYEPVWHVWQKLYPGDAVSLQGAARDMLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKV
VCSSPLCPAYRKEVVGLVDDRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLKDPALDLKV
IHLVRDPRAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGMGGPADYHALGAMEV
ICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSK
PFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC051963
ORF Size 1451 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC051963, AAH51963
RefSeq Size 2501 bp
RefSeq ORF 1451 bp
Locus ID 54371
Gene Summary Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...