Syn2 (BC085129) Mouse Tagged ORF Clone

CAT#: MG209143

  • TrueORF®

Syn2 (tGFP-tagged) - Mouse synapsin II (cDNA clone MGC:113746 IMAGE:5701861)

ORF Plasmid: DDK tGFP



  "BC085129" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2900074L19Rik; AI836018; AI841723
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209143 representing BC085129
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTCCTGAGGCGCCGGCTGTCGGACAGCAGCTTCATCGCCAACCTGCCCAATGGCTACATGACTG
ACCTTCAGCGCCCGGAGCCGCAGCAGCCCCCTCCTGCCCCTGGCCCCGGTGCCGCTACGGCGTCGGCGGC
CACCTCAGCTGCCTCGCCCGGTCCCGAGCGCAGGCCGCCACCCGCCCAGGCACCCGCGCCGCAGCCGGCG
CCGCAGCCCGCGCCGACGCCGTCGGTGGGCAGCAGCTTCTTCAGCTCGCTGTCGCAAGCGGTGAAGCAGA
CGGCCGCCTCCGCCGGCCTGGTGGACGCGCCCGCACCTTCGGCCGCTTCCAGGAAGGCCAAGGTGCTGCT
GGTGGTCGACGAGCCGCACACCGACTGGGCCAAATGCTTTCGGGGCAAAAAAATCCTTGGAGATTATGAC
ATCAAGGTGGAACAGGCAGAATTCTCAGAGCTTAACCTGGTGGCCCATGCAGATGGAACCTACGCCGTGG
ACATGCAGGTACTTCGGAATGGCACAAAGGTTGTCCGGTCCTTCAGACCAGACTTTGTGCTCATCCGACA
GCATGCATTTGGCATGGCAGAGAATGAAGACTTCCGCCACCTGGTCATTGGCATGCAGTACGCAGGCCTC
CCCAGCATCAACTCTCTGGAGTCTATTTACAACTTCTGTGACAAGCCGTGGGTGTTTGCTCAGATGGTGG
CCATCTTCAAGACACTGGGAGGGGAGAAATTCCCACTCATTGAGCAGACATACTACCCCAATCACCGAGA
GATGCTTACGCTTCCTACGTTCCCTGTGGTGGTGAAGATTGGCCATGCTCACTCAGGCATGGGCAAGGTC
AAAGTGGAAAATCACTACGACTTCCAGGACATTGCCAGTGTGGTGGCCCTCACCCAAACTTATGCCACGG
CAGAACCCTTCATCGACGCCAAGTATGACATCCGAGTTCAGAAGATTGGCAACAACTACAAGGCCTACAT
GAGGACATCCATCTCAGGGAACTGGAAGACAAACACTGGCTCGGCGATGCTGGAGCAAATTGCCATGTCG
GACCGGTACAAGCTCTGGGTAGATGCCTGCTCTGAAATGTTTGGTGGCCTGGACATCTGTGCAGTCAAAG
CCGTGCATGGCAAAGATGGCAAAGACTACATTTTTGAGGTCATGGACTGCAGCATGCCATTGATTGGGGA
ACACCAAGTGGAGGACAGACAACTCATCACTGATCTAGTCATCAGCAAGATGAACCAGCTATTGTCCAGG
ACTCCTGCCCTGTCTCCACAGAGACCTTTAACCACCCAACAGCCACAGAGTGGAACACTTAAGGAACCGG
ACTCCAGCAAGACCCCTCCTCAGCGGCCACCTCCCCAAGGGGGCCCTGGGCAACCCCAAGGAATGCAGCC
CCCAGGCAAGGTGCTGCCTCCACGCCGGCTCCCCTCGGGACCATCACTGCCATCTTCTTCCTCTTCCTCC
TCCTCTTCCTCCTCTTCTTCCTCGGCTCCTCAGAGGCCGGGCGGCCCCACCACCACCCACGGTGATGCAT
CCTCCAGCAGCAACTCCCTGGCAGAGGCCCAGGCACCTCAGGCTGCTCCAGCACAGAAGCCCCAGCCTCA
CCCACAGCTCAACAAGTCGCAGTCCCTGACAAATGCGTTCAGCTTCTCTGAGTCCTCCTTCTTCCGGTCT
TCAGCCAATGAAGATGAAGCCAAAGCGGAGACCATCCGGAGCTTGAGGAAGTCCTTTGCCAGCCTCTTTT
CAGAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209143 representing BC085129
Red=Cloning site Green=Tags(s)

MNFLRRRLSDSSFIANLPNGYMTDLQRPEPQQPPPAPGPGAATASAATSAASPGPERRPPPAQAPAPQPA
PQPAPTPSVGSSFFSSLSQAVKQTAASAGLVDAPAPSAASRKAKVLLVVDEPHTDWAKCFRGKKILGDYD
IKVEQAEFSELNLVAHADGTYAVDMQVLRNGTKVVRSFRPDFVLIRQHAFGMAENEDFRHLVIGMQYAGL
PSINSLESIYNFCDKPWVFAQMVAIFKTLGGEKFPLIEQTYYPNHREMLTLPTFPVVVKIGHAHSGMGKV
KVENHYDFQDIASVVALTQTYATAEPFIDAKYDIRVQKIGNNYKAYMRTSISGNWKTNTGSAMLEQIAMS
DRYKLWVDACSEMFGGLDICAVKAVHGKDGKDYIFEVMDCSMPLIGEHQVEDRQLITDLVISKMNQLLSR
TPALSPQRPLTTQQPQSGTLKEPDSSKTPPQRPPPQGGPGQPQGMQPPGKVLPPRRLPSGPSLPSSSSSS
SSSSSSSSAPQRPGGPTTTHGDASSSSNSLAEAQAPQAAPAQKPQPHPQLNKSQSLTNAFSFSESSFFRS
SANEDEAKAETIRSLRKSFASLFSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC085129
ORF Size 1755 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC085129.1
RefSeq Size 2563 bp
RefSeq ORF 1757 bp
Locus ID 20965
Gene Summary Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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