Cnot4 (BC048243) Mouse Tagged ORF Clone

CAT#: MG209679

  • TrueORF®

Cnot4 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 4 (cDNA clone MGC:54574 IMAGE:6493673)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,030.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Not4h, Not4hp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209679 representing BC048243
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGCAGTCCTGATGCAAAGGAAGACCCTGTGGAATGCCCTCTTTGCATGGAGCCCTTGGAAATAG
ATGATATTAACTTTTTCCCTTGCACCTGTGGCTACCAGATATGCCGATTTTGTTGGCATCGAATTCGCAC
TGATGAGAATGGGCTTTGTCCTGCATGTAGAAAACCATATCCAGAAGATCCAGCAGTTTACAAACCACTT
TCCCAGGAAGAATTACAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAAAGAAAACAGAAAATCT
CAGAAAATAGAAAACATTTGGCTAGTGTACGTGTTGTACAAAAAAACCTTGTGTTTGTTGTAGGTCTGTC
ACAGCGCCTAGCAGACCCAGAAGTTTTAAAACGACCAGAGTATTTTGGGAAGTTTGGTAAAATACATAAA
GTTGTCATCAATAATAGCACATCCTATGCAGGCTCACAGGGTCCGAGTGCCAGTGCTTATGTAACGTATA
TCCGGTCAGAAGATGCTCTCAGAGCAATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGGACACTAAA
GGCATCTTTAGGTACAACAAAGTACTGCAGTTATTTCTTAAAGAACATGCAGTGCCCAAAGCCTGACTGC
ATGTATCTTCATGAATTGGGAGATGAGGCAGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAGCACC
AAGAATATGAACAGAAGCTCCTCCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG
TTCAGTTGATAAGAATAAGAACAAAGTGACACCATTACAAAGCCCCATTGACAAACCTTCAGATTCTCTC
AGTATAGGGAATGGTGATAATTCCCAACAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTAT
CAAAATCCAACCCTGTCATCCCCATCAGTTCATCCAATCACAGTGCGAGGTCTCCTTTTGAAGGGGCAGT
AACAGAGTCACAGTCATTATTCTCAGACAATTTTCGCCACCCCAACCCTATACCAAGTGGGCTTCCTCCT
TTTCCCAGCTCTCCACAGACACCCAGTGATTGGCCTACAGCTCCAGAACCACAGAGCCTCTTCACATCAG
AAACAATCCCAGTTTCATCATCTACAGACTGGCAAGCAGCATTCGGCTTTGGTTCTTCTAAACAACCAGA
GGATGACTTGGGGTTTGACCCCTTTGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTA
TCCGTCCAAGATCAACCTTCCCTTTCCCCCACATCTCTTCAGAACGCCTCCTCACACACTACGACCGCCA
AAGGGCCAGGCTCTGGATTCCTGCACTCTGCTGCACCTACAAATGCCAACTCTCTCAATAGTACCTTTTC
AGTCTTGCCACAGAGGTTCCCTCAATTTCAGCAGCACCGAGCAGTTTATAATTCGTTCGGTTTTCCAGGC
CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC
CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC
CATAGCAGGTATTCCAGCATCTTCAGGAAACACTTTAGACTCTATTCAAGACGACAATCCTCCACACTGG
CTAAAATCCCTACAGGCCCTCACAGAGATGGACGGCCCCAGCGCTGCCTCGTCACAACCCCATCACAGCG
CCCCCTTCAGCACACAGATCCCTCTGCACAGAGCCAGTTGGAACCCCTACCCACCTCCTTCAAATCCCTC
CAGCTTCCACTCTCCACCCCCAGGCTTCCAGACAGCCTTCAGACCCCCCAGCAAAACCCCCACAGATCTG
CTACAGAGTTCCACACTGGACCGCCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209679 representing BC048243
Red=Cloning site Green=Tags(s)

MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL
SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK
VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC
MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQSPIDKPSDSL
SIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPP
FPSSPQTPSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKEL
SVQDQPSLSPTSLQNASSHTTTAKGPGSGFLHSAAPTNANSLNSTFSVLPQRFPQFQQHRAVYNSFGFPG
QAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPIAGIPASSGNTLDSIQDDNPPHW
LKSLQALTEMDGPSAASSQPHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPGFQTAFRPPSKTPTDL
LQSSTLDRH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC048243
ORF Size 1919 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC048243, AAH48243
RefSeq Size 3259 bp
RefSeq ORF 1919 bp
Locus ID 53621
Gene Summary Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...