Bbs2 (NM_026116) Mouse Tagged ORF Clone
CAT#: MG210279
- TrueORF®
Bbs2 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 2 homolog (human) (Bbs2)
ORF Plasmid: DDK
"NM_026116" in other vectors (4)
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CNY 7,030.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2410125H22Rik; AI447581 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210279 representing NM_026116
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCCTGTGTTCACTCTGAAACTGCGCCACAAAATCAGCCCCCGCATGGTGGCCATAGGGCGCT ACGACGGGACTCACCCGTGCTTGGCTGCCGCCACCCAAGCGGGCAAGGTTTTCATTCATAACCCTCACAC GCGGAGCCAGCATTTCAGTGCTTCCAGAGTGTTCCAGAGCCCTCTGGAGTCTGATGTCTCTCTGCTCAAC ATTAACCAGACGGTCAGCTGCCTGGGTTCGGGAGTCTTGAACCCTGAGCTTGGCTATGATACTCTTTTAG TGGGGACACAGACCAGTCTTTTGGCTTACGACATCTACAATAATTCAGATTTGTTCTACAGAGAGGTCTC AGATGGGGCAAATGCCATTGTGCTGGGGACCCTGGGAGACATTGCCCCACCTCTTGCAATCATCGGTGGA AACTGTGCTCTGCAGGGTTTCGATCACGAAGGAAATGATCTCTTTTGGACGGTTACTGGAGACAATGTGC ATTCCTTGGCTCTGTGTGACTTTGACGGTGATGGGAAGACAGAGCTTCTTGTTGGATCTGAAGATTTTGA CATTCGAGTTTTTAAAGAAGATGAGATTGTGGCAGAAATGACAGAGACAGAGATAGTCACCTCTCTGTGT CCTATGTATGGCAGTCGGTTTGGTTACGCCCTTTCCAATGGCACAGTTGGAGTTTATGACAAAACAGCCC GGTACTGGAGGATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATTAATTCTGATGGAGT GTGTGAACTGATAACCGGGTGGTCTAATGGGAAGGTTGATGCTCGCAGTGACCGGACTGGAGAGGTCATA TTTAAAGACAACTTCTCTTCTGCTGTTGCTGGTGTGGTAGAGGGAGATTACCGGATGGATGGCCACGTAC AGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAA CCTCCTGGACACAAGTGTGGAGCAGGACCTGATCCGAGAGCTGAGCCAGAAAAAGCAGAACCTGTTGCTA GAACTTCGCAACTATGAGGAGAGCACCAAGGCAGAACTGAGCAGTCCCCTGAACGAGGCCGATGGGCAGA AAGGCATAATCCCAGCCAATACCAGGCTCCACACAGCGCTCTCAGTCAATATGGGGAATGACTTACAAGA TGCGCATGCGGAATTAGGCATTTCCACTTCCAATGATACTATCATCCGAGCAGTACTAATTTTTGCAGAG GGAATTTTTGTGGGTGAAAGCCATGTGGTCCACCCCAGCATTCACAACCTTTCCAGCTCCCTCCGGGTAC CAATTACACCTCCCAAAGATGTCCCTGTGGATCTGCACCTGAAAACCTTCGTGGGTTACAGAAGCAGCAC CCAGTTCCATGTGTTTGAACTGACAAGACAGCTGCCCCGATTCACCATGTATGCATTGACAAGCCCAGAT GCAGCTAGCGAGCCTGTCAGCTACGTGAACTTCAGTGTTGCCGAGCGCACACAGAGGATGGTCACGTGGC TCAACCAGAACTTTCTGTTGCCAGAAGACAGTAATGTTCAGAATTCCCCGTTTCACGTCTGTTTCACATC GTTACGCAATGGTGGCCAGCTCTACATAAAAATGAAACAAAGTGGTGAGATCACTGTGAATACTGACGAT ATTGATCTAGCTGGTGACATCATCCAGTCGATCGCGTCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAG CAGATTTCCCTGTGTACTTCGAGGAACTACGGAAAGTGCTGGTGAAGGTGGACGAGTATCATTCAGTGCA TCAAAAGCTCAGCGCCGACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCAGAGGAT GCTCGGCTGATGAGGGACATGAAAACAATGAAGAGTCGTTACATGGAGCTCTATGACCTTAATAAAGATT TGCTGAATGGATACAAGATTCGCTGTAACAACCACACAGAACTGCTAGGAAACCTGAAGGCGGTGAACCA AGCAATCCAAAGAGCAGGCCGTCTGCGCGTTGGAAAGCCAAAGAACCAGGTGATCAGTGCTTGTCGGGAT GCGATTCGAAGCAACAACATCAACACCCTCTTCAGAATCATGCGAGTGGGCACTGCTCCTTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210279 representing NM_026116
Red=Cloning site Green=Tags(s) MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHTRSQHFSASRVFQSPLESDVSLLN INQTVSCLGSGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVSDGANAIVLGTLGDIAPPLAIIGG NCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKTELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLC PMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVI FKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQDLIRELSQKKQNLLL ELRNYEESTKAELSSPLNEADGQKGIIPANTRLHTALSVNMGNDLQDAHAELGISTSNDTIIRAVLIFAE GIFVGESHVVHPSIHNLSSSLRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELTRQLPRFTMYALTSPD AASEPVSYVNFSVAERTQRMVTWLNQNFLLPEDSNVQNSPFHVCFTSLRNGGQLYIKMKQSGEITVNTDD IDLAGDIIQSIASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADNSNLIRSLLVRAED ARLMRDMKTMKSRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVISACRD AIRSNNINTLFRIMRVGTAPS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026116 |
ORF Size | 2163 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026116.2, NP_080392.1 |
RefSeq Size | 2972 bp |
RefSeq ORF | 2166 bp |
Locus ID | 67378 |
UniProt ID | Q9CWF6 |
Gene Summary | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204609 | Bbs2 (untagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2), (10ug) |
CNY 5,288.00 |
|
MR210279 | Bbs2 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2) |
CNY 5,280.00 |
|
MR210279L3 | Lenti ORF clone of Bbs2 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2) |
CNY 8,270.00 |
|
MR210279L4 | Lenti ORF clone of Bbs2 (mGFP-tagged) - Mouse Bardet-Biedl syndrome 2 (human) (Bbs2) |
CNY 8,270.00 |