Smpd4 (BC026767) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4122402O22Rik; mKIAA1418
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210696 representing BC026767 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCCCTCACCTACCACAGCCTAGTTTCCTTCTGGCTAGCCTGAAAGCTGACTCTATAAATAAGC CCTTTGCACAACGTTGCCAAGATTTGGTGAAAGTCATTGAGGATTTTCCAGCCAAGGAATTGCATGCCGT CTTCCCATGGCTGGTGGAAAGCATCTTTGGCAGCCTGGATGGTGTCCTTGTTGGTTGGAATCTCCGATGC CTACAGGGACGTGTGAACCCTGTGGAGTACAGCACAGCGATGGAATTTCTAGACCCAAGTGGTCCAATGA TGAAGTTGGTTTATAAACTTCAAGCTGAAGACTATAACTTTGATTTTCCTGTCTCCTGTCTTCCTGGCCC TGTGAAGGCATCCATTCAAGAGAATGTCCTCCCTGACAGTCCTCTGTACCACAACAAAGTCCAGTTTCCC CCGACCGGAGGCCTTGGCCTGAACCTGGCCCTCAATCCATTTGAATACTATATGTTCTACTTTGCTTTGA GCCTCATCTCTCAAAAGCCAATGTCTATGACCCTCCATGTCCGTACTTCGGACTGTGCCTATTTCACCCT GGTGGATAGGTACCTATCATGGGTCCTACCCACTGAAGGCAGCGTACCTCCACCACTCTGCTCCAGTCCA GGAGGCTCCAGCCCCTCACCAGCTCCCAGAACACCAGCCATGCCCTTTGCCTCCTATGGCCTCCACACTA GCCTCTTGAAGCGACACATCTCTCATCAGACATCTGTGAATGCAGACCCTGCATCGCATGAGATCTGGCG GTCAGAAACTCTCCTCCAGGTTTTTGTGGAAATGTGGCTTCATCATTACTCCCTGGAGATGTACCAAAAA ATGCAGTCCCCTCATGCCAAGGAGTCCTTCACGCCTACTGAAGAGCACGTGCTGGTGGTACGCCTGCTGC TGAAGCACCTGCATGCCTTTGCCAACAGCCTGAAGCCAGATCAGGCTTCGCCATCTGCACACTCCCATGC CACCAGTCCGCTGGAGGAATTCAAACGGGCCGCAGTGCCTCGCTTTGTCCAGCAGAAGCTGTACGTCTTC CTGCAGCACTGTTTTGGCCACTGGCCTCTGGATGCAACCTTCAGAGCTGTCTTGGAGATGTGGCTGAGCT ACCTGCAGCCATGGAGGTATGCCCCTGAGAAACAGGCTCAGGGCAGCGATCCCCAGCCACGGTGTGTGTC AGAGAAGTGGGCACCCTTCATCCAGGAAAACCTGCTGATGTACACCAAGCTGTTTGTGAGCTTTCTGAAC CGTGCCCTACGCACAGACCTTGTCAGCCCCAAGAATGCACTCATGGTCTTCCGAGTGGCTAAGGTCTTTG CCCAGCCCAACCTGGCTGAGATGATCCAGAAAGGTGAGCAGCTGTTCCTGGAGCCGGAGCTCATCATCCC CCACCGCCAACACCGGCTCTTCACAGTCACCACCAGCTTCCTGTCACCATGGCCCCCTGTTGTGACAGAT GCCTCCTTCAAGGTGAAGAGCCATGTCTATAGCCTAGAGGGCCAAGACTGCAAATATACCCCAATGTTTG GTCCTGAAATCCGAACCCTGGTCTTGCGCCTTGCTCAGCTCATCACACAGGCCAAGCAGACTGCCAAGTC CATCTCTGATCAGTATGTGGAAAGCCCAACTGGCCGCTCCTTCCTGTCATGGCTGACCTTTGGCCTCACA GACACAAATAGCTGCTACCCAGCCAATGACCTGGATGAAATAGGTCAGGACAGCATCCGCAAGACAGACG AGTACTTAGAAAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCCCAGCTGGCCCAGCT CACGCTTGCCCTGGGGAGTGCTCGGGACGAGAATGGGAAGCAGCAGCTCCCAGATTGCATTGTGGGGGAA GAAGGACTCATCCTTACACCCTTGGGCCGCTACCAGATCATTAATGGTCTGCGAAGGTTCGAGATTGAGT ACCAGGGAGACTTAGAGCTGCAGCCCATCCGGAGCTACGAAATCACCAGTCTGGTCCGTGCACTCTTCCG GCTGTCCTCTGCCATCAACCGTAGATTTGCAGGCCAGATGGCAGCTCTGTGCTCCCGGAATGACTTCCTT GGCAGCTTCTGTCGCTACCACCTCACTGAGCCTGCCTTGAGCAACAGACATCTGCTGAGCCCTGTAGGGC GGAGGCAGGTCACCAATCCTGCACGGGGCCCCAGGCTCAGCCTCCGTTTCCTGGGCAGTTACCGGACACT GCTCCTGCTCCTGATGGCCTTCTTTGTGGCTTCTCTGTTCTGCATTGGGCCCCTGTCCTGCTCCTTGCTC CTGGTCCTAGGATACGTCCTCTATGCCATAGCTATGACACTGCTCACTGAACGGGGGAAGCTGCACCAGC TC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210696 representing BC026767 Red=Cloning site Green=Tags(s) MAFPHLPQPSFLLASLKADSINKPFAQRCQDLVKVIEDFPAKELHAVFPWLVESIFGSLDGVLVGWNLRC LQGRVNPVEYSTAMEFLDPSGPMMKLVYKLQAEDYNFDFPVSCLPGPVKASIQENVLPDSPLYHNKVQFP PTGGLGLNLALNPFEYYMFYFALSLISQKPMSMTLHVRTSDCAYFTLVDRYLSWVLPTEGSVPPPLCSSP GGSSPSPAPRTPAMPFASYGLHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQK MQSPHAKESFTPTEEHVLVVRLLLKHLHAFANSLKPDQASPSAHSHATSPLEEFKRAAVPRFVQQKLYVF LQHCFGHWPLDATFRAVLEMWLSYLQPWRYAPEKQAQGSDPQPRCVSEKWAPFIQENLLMYTKLFVSFLN RALRTDLVSPKNALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELIIPHRQHRLFTVTTSFLSPWPPVVTD ASFKVKSHVYSLEGQDCKYTPMFGPEIRTLVLRLAQLITQAKQTAKSISDQYVESPTGRSFLSWLTFGLT DTNSCYPANDLDEIGQDSIRKTDEYLEKALEYLRQIFRLSEAQLAQLTLALGSARDENGKQQLPDCIVGE EGLILTPLGRYQIINGLRRFEIEYQGDLELQPIRSYEITSLVRALFRLSSAINRRFAGQMAALCSRNDFL GSFCRYHLTEPALSNRHLLSPVGRRQVTNPARGPRLSLRFLGSYRTLLLLLMAFFVASLFCIGPLSCSLL LVLGYVLYAIAMTLLTERGKLHQL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026767
ORF Size	2384 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026767, AAH26767
RefSeq Size	3046 bp
RefSeq ORF	2384 bp
Locus ID	77626
Gene Summary	Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. May sensitize cells to DNA damage-induced apoptosis.[UniProtKB/Swiss-Prot Function]

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