Ppp4r1 (BC029761) Mouse Tagged ORF Clone

CAT#: MG211287

  • TrueORF®

Ppp4r1 (tGFP-tagged) - Mouse protein phosphatase 4, regulatory subunit 1 (cDNA clone MGC:28197 IMAGE:3989055)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,890.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Pp4r1, MGC31567
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211287 representing BC029761
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACCTCTCCCTGCTCCAGGAGGACCTGCCGGAGGACGCGGACGGACTTGATGTGGATGACTACA
GCTCAGAGTCTGATGTGATTATTATACCTTCAGCCCTGGACTTCGTCTCACAAGATGAAATGTTGACACC
CTTGGGGAGGCTGGACAAGTATGCTGCAAGTGAGAACGTATTTAACAGACAAATGGTGGCCCGGAGTTTG
CTGGATACTCTGAGGGAAGTCTGCGATGATGAGAGAGACTGCATTGCTGTCTTGGAAAGAATTAGCCGAT
TAGCTGATGACTCCGAACCAACTGTGAGAGCGGAGCTGATGGAACAAGTGCCGCACATTGCACTGTTTTG
TCAAGAGAACCGGCCTTCTATACCGTATGCTTTTTCCAAGTACTTACTACCAATTGTGGTCAGATACCTC
GCAGACCAGAATAACCAGGTGAGGAAAACCAGTCAGGCAGCTTTGCTGGCTCTGCTGGAGCAGGAACTGA
TTGAGCGATTTGACGTGGAGACCAAGGTGTGCCCTGTCCTCATAGACCTGACTGCCCCAGACAGCAATGA
TGATGTGAAGACAGAGGCTGTGGCTATAATGTGCAAGATGGCCCCCATGGTTGGGAAGGACATTACGGAG
CGCCTCATCCTCCCCAGGTTCTGCGAGATGTGCTGTGACTGTCGAATGTTTCATGTCCGGAAGGTCTGCG
CTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAACAAGCAACAGAAGAAATGTTGCTGCCCAGGTT
CTTCCAGCTGTGCTCTGATAACGTGTGGGGCGTCCGAAAGGCTTGTGCTGAGTGCTTCATGGCTGTCTCC
TGTGCAACATGTCAAGAAATCCGACGGACAAAGTTATCAGCACTATTTATTAACTTGATCAGTGATCCTT
CACGTTGGGTTCGCCAAGCAGCCTTTCAGTCTCTGGGGCCTTTCATATCCACGTTTGCTAATCCATCAAG
CTCGGGCCAGTGCTTTAAAGATGAGAGCAAGAGCTCGGAAGACTCCTCGGCAGAAGACAAAGACAGGATG
AGAGACAGTGATGTCGTAGAAGAAGAGCATCGCAGGCCAGAGGACGCTCCTTCAGACCTCAGTGCCCCTC
ACCCCAGCGCCAGGCTGGAGAGCCTCGAAGGCTGTGCTGCCAAGACACCTGGGCACTCTGCAGGTGACGT
GCCTGCTCCAGTGGACAGCTCCTTACTCTGTACTTTGTCCTCAGAGTCTCCTCAGGAAGCAGCTAGCAAT
GATGAGAATGGTAGAAAGCCTGACACCAACAGCAAGTCTGCGTCCCGGCCAGACGCTGGCACCAGCTCCC
CAGAGGCCACTCCCTTAGACCAGGACATGTTCAACTCCTTCCATTTCTGGAGGACTCCTCTACCTAAAAT
AGATCTTGATAAAGAGCTCCAGCAGGACCCCGAGGAGAGACTCAGCCCAGAGAGAACAGGAGATGTACCT
GCAGCCCCTCTACCAGGCCCTCCCAATATCACCATGGCTACCCGGAAGGAACTAGAAGAAATGATAGAAA
ACCTTGAGCCACACATGGATGACCCGGATGTGAAAGCACAGGTGGAAGTGCTGTCGGCTGCCCTGCGAGC
TTCCAGCCTGGATGCTCACGAAGAGACCGGTGGTGTAGAGCAGCGGAGTGAGCTACAAGATGAAGTGTGT
GTCAGTGAGCTTCCAGACTGTAACATAAGTCACGACACTTGTGTGCCTTTGATCAGCGCTGCTGAGGAGA
ATGTGGAGGCCACTCCTGATTATGTCCATGGAGGTGCAGACGTAAGCCCTGGTGATGGCTTCAGCCCGGA
TGAAGATAGGAGACCCAAAGTCCAGGATGTTGTACCACAAGCGTTACTGGATCAGTACTTGTCAATGACC
GACCCTTCTCGAGCACAGACAGTTGACACTGAGATCGCTAAGCACTGTGCATACAGTCTGCCGGGTGTGG
CCCTGACTCTTGGCAGACAGAACTGGCACTGTTTGAGGGAGACTTATGAGACCCTAGCGTCGGACATGCA
GTGGAAAGTTCGAAGGACTCTAGCCTTCTCCATCCATGAGCTTGCAGTGATCCTTGGGGACCAGCTGACA
GCTGCAGACCTGGTTCCAATCTTCAATGGATTTTTAAAAGACCTCGATGAAGTCAGGATAGGTGTTCTTA
AACACTTGCATGACTTTCTGAAGCTTCTTCACATTGATAAAAGAAGAGAGTACCTTTATCAGCTCCAGGA
GTTTTTGGTCACAGACAACAGTAGAAATTGGCGCTTTCGAGCTGAACTGGCAGAACAGCTGATTTTACTT
CTGGAGTTATACAGTCCCAGAGATGTTTATGATTACCTACGACCCATCGCTCTGAATCTGTGTGCAGACA
AAGTTTCTTCAGTCCGCTGGATTTCCTACAAGTTGGTCAGTGAAATGGTGAAGAAACTCCACACAGCAAC
ACCACCAACCTTCGGAGTAGATCTCATCAATGAGCTGGTAGAGAATTTTGGCAGGTGTCCAAAATGGTCT
GGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAAGACGACTGCCTCCCCATGGACCAGTTTG
CTGTGCACTTGATGCCACATTTGCTGACCTTGGCAAATGACAGGGTTCCCAATGTTAGAGTGCTGCTTGC
GAAAACCCTGCGACAGACTCTGCTGGAGAAAGAGTACTTTTTAGCCTCTGCCAGCTGTCACCAGGAGGCC
GTGGAGCAGACAATCATGGCCCTGCAGATGGACCGAGACAGTGATGTCAAGTACTTTGCAAGCATCCACC
CCTCCAGTACCAAACTCTCTGAAGATGCAATGAGCACAGCTTCCTCCACCTAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211287 representing BC029761
Red=Cloning site Green=Tags(s)

MADLSLLQEDLPEDADGLDVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENVFNRQMVARSL
LDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKYLLPIVVRYL
ADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIDLTAPDSNDDVKTEAVAIMCKMAPMVGKDITE
RLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVS
CATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQCFKDESKSSEDSSAEDKDRM
RDSDVVEEEHRRPEDAPSDLSAPHPSARLESLEGCAAKTPGHSAGDVPAPVDSSLLCTLSSESPQEAASN
DENGRKPDTNSKSASRPDAGTSSPEATPLDQDMFNSFHFWRTPLPKIDLDKELQQDPEERLSPERTGDVP
AAPLPGPPNITMATRKELEEMIENLEPHMDDPDVKAQVEVLSAALRASSLDAHEETGGVEQRSELQDEVC
VSELPDCNISHDTCVPLISAAEENVEATPDYVHGGADVSPGDGFSPDEDRRPKVQDVVPQALLDQYLSMT
DPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLT
AADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILL
LELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHTATPPTFGVDLINELVENFGRCPKWS
GRQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKEYFLASASCHQEA
VEQTIMALQMDRDSDVKYFASIHPSSTKLSEDAMSTASSTY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC029761
ORF Size 2855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC029761, AAH29761
RefSeq Size 3739 bp
RefSeq ORF 2855 bp
Locus ID 70351
Gene Summary Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...