Fbln2 (BC005443) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730577E14Rik; FIBL-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211752 representing BC005443 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTCCAGGAATCTGCAGGAGTGTGGCTGGCCTTGGCTCTGGTCACAGCTCTGACTCCGAGCCCAA GCATGGCCGTCCCGTGGCAGGATTGCACAGGTGCTGAGTGTCCCCTGCTGGAGAACTGCATCGAGGAGGC GTTGGAGCCAGGAGCCTGCTGTGCTACCTGTGTGCAGCAGGGCTGTGCCTGCGAAGGCTACCAGTACTAC GACTGCGTACAAGGAGGGTTTGTGGATGGCCGTGTACCAGCCGGCCAGTCCTACTTTGTGGACTTTGGCA GCACCGAGTGCTCCTGCCCACCCGGTGGTGGCAAGATCAGCTGCCAGTTCATGCTGTGCCCTGAGCTACC ACCCAACTGCATTGAAGCTGTAGTAGTAGCCGACAGCTGCCCGCAGTGTGGCCAGGTGGGCTGTGTCCAC AGTGGCCGTAAGTATGCTGCTGGCCATACCGTCCACCTCTCATCCTGCCGGGCTTGCCACTGTCCTGACG CTGGTGGTGAGCTCATTTGCTACCAGCTTCCTGGTTGCCATGGGAACTTCTCGGATGCTGAGGAGGGTGA CTCTGAGAGGCAGTATGAAGACCCCTACAGCTATGACCAGGAGGTGGCAGAGGCAGAAGCTACTACGGCC ATTGTGAACGAGGTCCAGGCAGGTGCAGAGGGCCCCCCAGCTGCTCTGGGAGGAGGGAATCTGCCACCGT CCTCCATCAGAGTAACACCCTGGCCAGTTGCCCTTCCCAGACCCACAGCAGCTGCTGCCCTGGGTCCCCC AGCACCTGTGCAGGCCAAAGCCAGGAGAGTGACATTTGACACTGAAGAGGATGAGGAGGAAGAAGAGGAG GAGACACTAGTCACTGAGCCACCAACAGCAGGCAGTCCTGGAAGGCTGGACAGCCTGCCCACAAGATCCC CAGCCAGACCTGGTTTCCCTGTCCAGGAGAAGGAGGCTGAAGCTAAGGCAGGGCCTGAGGAAAACCTTAT TCCTGATGCCCAGGTCACTCCTCGAAGTGTCATGCAGGAGGGTGCTGCACCTCTGCCAAGGTCAGGCCTG GCTGCTCTCAGTCCATCTCTGGCCACTGACAGCTCTTCAGAGGACCCAGTAAAGCCCAGCGGTCACCCCA CTCTATCTACACTGCCACCTGACCGAGCCCAGGTCTCCCCCTCCCCAGAGACACCTGAAGAAATACCCCA GCATCCACAGTTGCTACCTCGATTCCGGGCAGAAGAAGACATAGACCCCAACTCAGTACATTCTGTCCCC AGAGGTGATCTTGATGGCTCCACCAAGGACCTAATCGAGACCTGTTGTGCAGCGGGCCAGCAGTGGGCCA TCGACAATGATGAGTGCCAGGAGATCCCCGAGAATGGTGCCCAGAGTGACATCTGTAGGATAGCCCAGAG GCAATGCTGCATCTCCTACTTGAAAGAGAAGAGCTGTGTGGCTGGGGTCATGGGAGCCAAGGAGGGTGAG ACCTGTGGAGCAGAGGACAATGATACCTGCGGCGTCTCACTCTACAAGCAATGCTGTGACTGCTGTGGCC TGGGACTCCGAGTGCGGGCTGAGGGCCAGTCGTGTGAGTCCAACCCCAACCTTGGCTATCCCTGCAACCA TGTCATGCTCTCCTGCTGTGAAGGTGAAGAGCCCCTCATAGTGCCTGAGGTCCGCCGGCCACCAGAGCCC GAGGCTGCTCCTCGCAGAGTTTCAGAGACGGAGATGGCAAGCCGGGAGGCCCTGTCACTGGGCACAGAGG CTGAACTGCCCAATAGCCTGCCGGGAGATGACCAGGATGAGTGCCTGATGCTCCCTGGGGAGCTTTGCCA GCATCTTTGCATCAACACCGTCGGCTCCTACCGCTGTGCCTGCTTCCCTGGATTCGAGCTGCAGGGTGAT GGCCGTACCTGCCGCCCAGATAGAGGTGCCCCACAGTTGGACACTGCACGAGAGTCTGCACCGAGGTCTG AGTCTGCCCAGGTATCTCCCAACACCATCCCACTGCCGGTGCCACAGCCCAACACCTGCAAAGACAATGG GCCCTGCCGTCAGGTGTGTCGTGTTGTTGGGGACACAGCTATGTGCTCCTGTTTCCCTGGCTACGCCATC ATGGCAGATGGTGTGTCCTGTGAAGACATCAATGAGTGTGTGACAGACCTGCACACGTGCACCCGGGCCG AGCACTGTGTGAACACACCAGGTTCCTTCCAATGCTACAAGGCGCTCACCTGTGAGCCAGGCTATGTCCT CACAGATGGTGAATGCACAGACGTGGACGAATGTGTGACGGGCACACACAACTGCCAGGCAGGCTTCTCA TGCCAGAACACCAAGGGCTCCTTCTACTGCCAGGCCCGGCAACGCTGCATGGATGGCTTCTTGCAGGACC CCGAGGGCAACTGTGTGGACATCAACGAATGCACATCGTTACTGGAGCCTTGCCGCTCAGGGTTCAGCTG CATCAACACAGTGGGCTCCTACACCTGTCAGAGGAACCCACTGGTCTGCGGTCGCGGTTACCATGCTAAC GAGGAGGGCTCTGAATGTGTGGATGTGAATGAGTGTGAGACAGGTGTGCATCGCTGTGGCGAGGGCCAAC TGTGCTATAACCTCCCTGGATCCTACCGCTGTGACTGCAAGCCTGGCTTCCAGAGGGATGCATTCGGCAG GACTTGCATTGATGTGAACGAATGCTGGGTCTCGCCGGGCCGCCTGTGCCAGCACACATGTGAGAACACA CCGGGCTCCTACCGCTGCTCCTGCGCTGCTGGCTTCCTTTTGGCCGCAGATGGCAAACATTGTGAAGATG TGAACGAGTGCGAGACTCGGCGCTGCAGCCAGGAATGTGCCAACATCTATGGCTCCTATCAGTGCTACTG CCGTCAGGGCTACCAGCTGGCAGAGGATGGGCATACCTGCACAGACATCGATGAGTGTGCACAGGGCGCG GGCATTCTCTGTACCTTCCGCTGTGTCAACGTGCCTGGGAGCTACCAGTGTGCATGCCCAGAGCAGGGGT ATACAATGATGGCCAACGGGAGGTCCTGCAAGGACCTGGATGAGTGTGCACTGGGCACCCACAACTGCTC TGAGGCTGAGACCTGCCACAATATCCAGGGGAGTTTCCGCTGCCTGCGCTTTGATTGTCCACCCAACTAT GTCCGTGTCTCAGAAACGAAGTGCGAGCGCACCACATGCCAGGATATCACGGAATGTCAAACCTCACCAG CTCGCATCACGCACTACCAGCTCAATTTCCAGACAGGCCTACTGGTACCTGCACATATCTTCCGCATCGG CCCTGCTCCCGCCTTTGCTGGGGACACCATCTCCCTGACCATCACGAAGGGCAATGAGGAGGGCTACTTC GTCACACGCAGACTCAATGCCTACACTGGTGTGGTATCCCTGCAGCGGTCTGTTCTGGAGCCGCGGGACT TTGCCCTAGATGTGGAGATGAAGCTTTGGCGCCAGGGCTCTGTCACTACCTTCCTGGCCAAGATGTACAT CTTCTTCACCACTTTTGCCCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211752 representing BC005443 Red=Cloning site Green=Tags(s) MMLQESAGVWLALALVTALTPSPSMAVPWQDCTGAECPLLENCIEEALEPGACCATCVQQGCACEGYQYY DCVQGGFVDGRVPAGQSYFVDFGSTECSCPPGGGKISCQFMLCPELPPNCIEAVVVADSCPQCGQVGCVH SGRKYAAGHTVHLSSCRACHCPDAGGELICYQLPGCHGNFSDAEEGDSERQYEDPYSYDQEVAEAEATTA IVNEVQAGAEGPPAALGGGNLPPSSIRVTPWPVALPRPTAAAALGPPAPVQAKARRVTFDTEEDEEEEEE ETLVTEPPTAGSPGRLDSLPTRSPARPGFPVQEKEAEAKAGPEENLIPDAQVTPRSVMQEGAAPLPRSGL AALSPSLATDSSSEDPVKPSGHPTLSTLPPDRAQVSPSPETPEEIPQHPQLLPRFRAEEDIDPNSVHSVP RGDLDGSTKDLIETCCAAGQQWAIDNDECQEIPENGAQSDICRIAQRQCCISYLKEKSCVAGVMGAKEGE TCGAEDNDTCGVSLYKQCCDCCGLGLRVRAEGQSCESNPNLGYPCNHVMLSCCEGEEPLIVPEVRRPPEP EAAPRRVSETEMASREALSLGTEAELPNSLPGDDQDECLMLPGELCQHLCINTVGSYRCACFPGFELQGD GRTCRPDRGAPQLDTARESAPRSESAQVSPNTIPLPVPQPNTCKDNGPCRQVCRVVGDTAMCSCFPGYAI MADGVSCEDINECVTDLHTCTRAEHCVNTPGSFQCYKALTCEPGYVLTDGECTDVDECVTGTHNCQAGFS CQNTKGSFYCQARQRCMDGFLQDPEGNCVDINECTSLLEPCRSGFSCINTVGSYTCQRNPLVCGRGYHAN EEGSECVDVNECETGVHRCGEGQLCYNLPGSYRCDCKPGFQRDAFGRTCIDVNECWVSPGRLCQHTCENT PGSYRCSCAAGFLLAADGKHCEDVNECETRRCSQECANIYGSYQCYCRQGYQLAEDGHTCTDIDECAQGA GILCTFRCVNVPGSYQCACPEQGYTMMANGRSCKDLDECALGTHNCSEAETCHNIQGSFRCLRFDCPPNY VRVSETKCERTTCQDITECQTSPARITHYQLNFQTGLLVPAHIFRIGPAPAFAGDTISLTITKGNEEGYF VTRRLNAYTGVVSLQRSVLEPRDFALDVEMKLWRQGSVTTFLAKMYIFFTTFAP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC005443
ORF Size	3522 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005443, AAH05443
RefSeq Size	3969 bp
RefSeq ORF	3524 bp
Locus ID	14115
Gene Summary	Its binding to fibronectin and some other ligands is calcium dependent. May act as an adapter that mediates the interaction between FBN1 and ELN.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.