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Phactr1 (NM_198419) Mouse Tagged ORF Clone
CAT＃: MG213383
- TrueORF®
Phactr1 (tGFP-tagged) - Mouse phosphatase and actin regulator 1 (Phactr1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_198419" in other vectors (4)
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CNY 6,432.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9630030F18Rik; Rpel1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG213383 representing NM_198419
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTATCCCAAAATGGATTATTTTCTGGACGTTGAGTCTGCTCACAGACTCTTGGATGTGGAGTCGG CGCAGAGGTTCTTCTACAGTCAAGGAGCTCAAGCTCGCCGGGCGACCCTGCTCCTGCCTCCCACATTAAT GGCGGCTTCTTCGGAGGATGACATAGACCGGCGGCCCATCAGAAGAGTCCGCTCCAAGAGCGACACGCCA TATCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCCGAGGAAGTGGAGAGGCTGGCAGCCATGC GTTCCGACTCGCTGGTCCCAGGTACGCACACCCCGCCCATCCGGAGGAGGAGTAAGTTTGCCAACCTGGG GAGGATTTTCAAACCCTGGAAATGGAGGAAGAAGAAAAGTGAAAAGTTCAAGCACACGTCAGCAGCTCTG GAAAGGAAGATTTCGATGAGGCAGAGCAGAGAGGAGCTGATCAAGAGAGGGGTCTTGAAGGAGATCTACG ATAAAGATGGAGAACTCTCTATATCAAATGAAGATGACTCCCTGGAGAACGGACAGTCCCTGAGCTCCAG CCAACTCTCTTTGCCTGCTCTGTCGGAAATGGAGCCTGTCCCAATGCCCAGGGACCCATGCTCATATGAG GTGCTCCAAGCTTCAGACATTATGGATGGACCAGTGTCTGAAGAGAGTCCCTCTGCCAGTGAGTCTGGAG TCCTCCTGTCCCAAGATCCTTCAGCCAAACCAGTTCTGTTCCTGCCCCCCAAAAAATCTGCTGCTTTCCC TGGAGACCATGAGGAGACCCCAGTGAAACAGTTGTCCCTTCACAAGCAGCCCCCGGCCCTGCCTCCCAAA CCCACTGCCCGGATTGCCAACCACTTAACAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCAGTGA AACTGTCGCCTCCGCTACCTCCAAAGAAAGTCCTGATCTGTATGCCTGTAGGGGGGCCAGAGCTCACTCT GGCATCCTATGCAGCCCAGAAGAGCAGCCAGCAGGCCGTGGCCCAGCACCACCACACCGTCCTACCATCC CAGATGCAGCACCAGCTGCAGTATGGCAGTCACGGCCAGCATCTCCCATCCTCCACCGGCACCTTGCCCA TGCACCCCTCAGGCTGCAGGATGATCGACGAGCTGAACAAGACACTCGCCATGACGATGCAGAGGCTGGA AAGCTCCGAGCAACGAGTCCCCTGTTCCACGTCTTACCACAGTTCTGGTTTGCACTCAAGCGACGGCATC ACAAAAGCAGGACCCATGGGCCTTCCGGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGATGACA ATAAGGAAAATGTGCCTCATGAACCCGACTATGAAGACTCGCCCTGCCTGTATGGCCGGGAGGAGGAGGA AGAGGAGGAAGATGAAGATGATGACGCCTCTTTGTACACCAGCTCACTGGCCATGAAGGTGTGCAGGAAG GACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCTAAGCGAGAGCTAGAAGAAAAGAACATCCTCCCCA GACAGACGGATGAGGAACGGCTGGAGCTCAGACAGCAGATTGGCACCAAGCTCACCAGGCGGCTGAGCCA GAGACCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAGCCTCGGAATGAACAAGAAGAACAGGAG GAGAAGCGGGAAATCAAGAGGAGGCTGACTCGCAAGCTCAGCCAAAGGCCCACAGTGGAAGAACTCCGGG AGAGGAAGATCCTTATCCGCTTCAGTGACTACGTGGAAGTGGCAGATGCCCAAGACTATGACCGAAGGGC AGACAAGCCATGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATTCGGAAGGAACTCAATGAGTTTAAA AGTACCGAGATGGAAGTCCACGAGTTAAGTAGACACTTAACCAGGTTTCATCGACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG213383 representing NM_198419
Red=Cloning site Green=Tags(s) MDYPKMDYFLDVESAHRLLDVESAQRFFYSQGAQARRATLLLPPTLMAASSEDDIDRRPIRRVRSKSDTP YLAEARISFNLGAAEEVERLAAMRSDSLVPGTHTPPIRRRSKFANLGRIFKPWKWRKKKSEKFKHTSAAL ERKISMRQSREELIKRGVLKEIYDKDGELSISNEDDSLENGQSLSSSQLSLPALSEMEPVPMPRDPCSYE VLQASDIMDGPVSEESPSASESGVLLSQDPSAKPVLFLPPKKSAAFPGDHEETPVKQLSLHKQPPALPPK PTARIANHLTDPGAPVKLPCLPVKLSPPLPPKKVLICMPVGGPELTLASYAAQKSSQQAVAQHHHTVLPS QMQHQLQYGSHGQHLPSSTGTLPMHPSGCRMIDELNKTLAMTMQRLESSEQRVPCSTSYHSSGLHSSDGI TKAGPMGLPEIRQVPTVVIECDDNKENVPHEPDYEDSPCLYGREEEEEEEDEDDDASLYTSSLAMKVCRK DSLAIKLSNRPSKRELEEKNILPRQTDEERLELRQQIGTKLTRRLSQRPTAEELEQRNILKPRNEQEEQE EKREIKRRLTRKLSQRPTVEELRERKILIRFSDYVEVADAQDYDRRADKPWTRLTAADKAAIRKELNEFK STEMEVHELSRHLTRFHRP TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198419 |
| ORF Size | 1947 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198419.3, NP_940811.2 |
| RefSeq Size | 5682 bp |
| RefSeq ORF | 1950 bp |
| Locus ID | 218194 |
| Gene Summary | Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization (PubMed:30256902).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220117 | Phactr1 (untagged) - Mouse phosphatase and actin regulator 1 (Phactr1), transcript variant 1, (10ug) |
CNY 8,360.00 |
|
| MR213383 | Phactr1 (Myc-DDK-tagged) - Mouse phosphatase and actin regulator 1 (Phactr1), transcript variant 1 |
CNY 4,832.00 |
|
| MR213383L3 | Lenti ORF clone of Phactr1 (Myc-DDK-tagged) - Mouse phosphatase and actin regulator 1 (Phactr1), transcript variant 1 |
CNY 5,610.00 |
|
| MR213383L4 | Lenti ORF clone of Phactr1 (mGFP-tagged) - Mouse phosphatase and actin regulator 1 (Phactr1), transcript variant 1 |
CNY 5,610.00 |
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