Stk10 (NM_009288) Mouse Tagged ORF Clone
CAT#: MG220805
- TrueORF®
Stk10 (tGFP-tagged) - Mouse serine/threonine kinase 10 (Stk10), (10ug)
ORF Plasmid: DDK
"NM_009288" in other vectors (4)
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CNY 7,890.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Gek1; L; Lok; mKIAA4026 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG220805 representing NM_009288
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCGCCAATTTCCGCCGCATCTTGCGGTTATCCACCTTCGAGAAGAGAAAGTCCCGTGAATATG AGCACGTCCGCCGGGACCTGGACCCCAACGATGTATGGGAGATCGTGGGCGAGCTGGGAGACGGCGCCTT CGGCAAAGTCTACAAGGCCAAAAACAAGGAGACCGGTGCCCTGGCAGCAGCCAAAGTCATAGAGACCAAG AGCGAGGAGGAGCTGGAGGACTACATCGTGGAGATAGAGATCCTGGCCACCTGTGACCACCCGTATATCG TGAAGCTCCTGGGGGCCTATTACTATGATGGGAAGCTCTGGATCATGATTGAGTTCTGCCCTGGGGGAGC TGTGGATGCCATCATGCTGGAGCTGGATCGAGGCCTCACTGAGCCCCAGATTCAGGTCGTATGCCGCCAG ATGTTGGAGGCTCTCAACTTCCTGCACGGCAAGAGGATCATCCACCGAGACCTGAAAGCTGGCAACGTGC TTATGACCCTTGAGGGGGACATCAGGCTGGCTGATTTTGGTGTGTCTGCCAAGAATCTGAAAACTCTGCA AAAGCGAGACTCCTTCATAGGGACACCTTACTGGATGGCCCCTGAGGTGGTGCTGTGTGAAACCATGAAG GACGCCCCCTATGACTACAAGGCTGACATCTGGTCCTTGGGCATCACGCTGATCGAGATGGCGCAGATCG AGCCCCCACACCACGAGCTCAACCCCATGCGGGTCCTGCTCAAGATTGCCAAGTCGGACCCTCCTACCCT GCTTACACCCTCCAAGTGGTCTGTGGAGTTCCGAGACTTTCTGAAGATAGCCTTGGATAAGAACCCAGAA ACCCGGCCCAGTGCTGCACAGCTGCTGCAGCATCCCTTCGTCAGCAGAGTCACCAGCAATAAGGCTCTTC GGGAGCTGGTGGCCGAGGCAAAGGCGGAGGTAATGGAGGAGATTGAGGATGGCAGGGAGGACGGAGAAGA GGAGGATGCTGTGGATGCTGTTCCGCCCCTGGTCAACCACACTCAGGACTCTGCCAATGTAACTCAGCCA AGCCTCGACTCCAACAAGCTTCTCCAGGATTCTTCCACCCCCCTGCCTCCCAGCCAGCCTCAGGAGCCTG TGAACGGGCCCTGTAGCCAACCCTCTGGGGATGGACCCCTCCAAACCACCAGCCCTGCAGATGGGCTCTC CAAGAATGACAATGACTTAAAGGTGCCTGTTCCCCTCCGGAAGTCCCGGCCACTGTCCATGGATGCCAGA ATTCAGATGGATGAAGAGAAACAAATCCCTGACCAGGATGAGAACCCGAGTCCTGCAGCCAGCAAGTCCC AAAAGGCCAACCAGAGCCGGCCTAACAGCAGTGCCCTGGAGACTTTGGGTGGTGAGGCTCTGACCAACGG TGGCCTGGAACTCCCCAGCTCTGTCACTCCAAGCCATTCTAAGAGGGCCTCGGACTGTAGCAATCTGTCT ACCTCAGAGAGCATGGACTATGGCACCTCCCTGTCTGCTGACCTGTCACTGAACAAAGAAACGGGCTCAT TGTCTCTCAAGGGCTCAAAACTGCACAATAAGACCCTGAAACGGACCCGCCGGTTTGTGGTGGATGGTGT GGAGGTGAGCATCACCACCTCCAAGATCATCAGCGAGGATGAGAAGAAAGACGAGGAGATGAGATTTCTC AGGCGCCAGGAGCTCCGAGAGCTTCGGCTTCTGCAGAAAGAAGAACATCGGAACCAGACGCAGCTGAGCA GCAAGCACGAGCTGCAGCTGGAGCAGATGCACAAACGTTTTGAACAAGAAATCAACGCCAAGAAGAAATT CTATGACGTGGAGCTAGAAAACCTGGAGCGGCAGCAGAAGCAGCAGGTAGAGAAGATGGAGCAGGACCAC AGCGTGCGCCGCAAAGAGGAGGCCAAGCGGATCCGCCTGGAGCAGGATCGAGACTACGCCAAGTTCCAAG AGCAACTCAAGCAAATGAAGAAGGAGGTGAAGAGTGAGGTTGAGAAACTGCCCCGGCAGCAGCGGAAGGA GAGCATGAAGCAGAAGATGGAGGAACACTCTCAGAAGAAACAGCGGCTGGACCGGGACTTTGTAGCCAAG CAGAAGGAGGACCTGGAGCTGGCCATGAGGAAGCTCACCACGGAAAACAGGCGCGAGATCTGTGACAAGG AGCGAGATTGCCTTAGCAAGAAGCAGGAGCTCCTCCGAGACCGTGAGGCGGCCCTGTGGGAGATGGAGGA GCACCAGTTGCAGGAGCGACATCAGCTGGTGAAGCAGCAACTGAAGGACCAGTACTTCCTGCAGCGGCAT GACCTGCTGCGTAAGCATGAGAAGGAGCGGGAACAGATGCAGCGCTACAACCAGCGCATGATGGAGCAGC TGAAGGTCAGACAGCAACAGGAGAAGGCGCGGCTACCCAAGATCCAGAGGAGCGACGGCAAGACGCGCAT GGCCATGTACAAGAAGAGCCTGCACATCAACGGTGCGGGCAGTGCCTCTGAGCAGCGCGAGAAGATCAAG CAGTTCTCCCAGCAGGAGGAGAAGAGGCAGAAGGCGGAGAGGCTGCAGCAGCAGCAGAAACACGAGAACC AGATGCGAGACATGGTGGCGCAGTGTGAAAGCAACATGAGCGAGCTACAGCAGCTGCAGAATGAAAAGTG TCATCTGTTGGTAGAGCATGAAACCCAGAAGCTGAAGGCCCTGGATGAGAGCCACAACCAGAGCCTGAAG GAATGGCGAGACAAGCTTCGGCCACGCAAAAAGGCCCTGGAAGAGGATTTGAACCAGAAGAAGCGGGAAC AGGAAATGTTCTTCAAATTAAGCGAGGAGGCAGAGCCCAGACCCACCACACCCAGCAAAGCCAGCAACTT CTTCCCCTACAGCTCTGGGGATGCTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220805 representing NM_009288
Red=Cloning site Green=Tags(s) MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNDVWEIVGELGDGAFGKVYKAKNKETGALAAAKVIETK SEEELEDYIVEIEILATCDHPYIVKLLGAYYYDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQ MLEALNFLHGKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVLCETMK DAPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPE TRPSAAQLLQHPFVSRVTSNKALRELVAEAKAEVMEEIEDGREDGEEEDAVDAVPPLVNHTQDSANVTQP SLDSNKLLQDSSTPLPPSQPQEPVNGPCSQPSGDGPLQTTSPADGLSKNDNDLKVPVPLRKSRPLSMDAR IQMDEEKQIPDQDENPSPAASKSQKANQSRPNSSALETLGGEALTNGGLELPSSVTPSHSKRASDCSNLS TSESMDYGTSLSADLSLNKETGSLSLKGSKLHNKTLKRTRRFVVDGVEVSITTSKIISEDEKKDEEMRFL RRQELRELRLLQKEEHRNQTQLSSKHELQLEQMHKRFEQEINAKKKFYDVELENLERQQKQQVEKMEQDH SVRRKEEAKRIRLEQDRDYAKFQEQLKQMKKEVKSEVEKLPRQQRKESMKQKMEEHSQKKQRLDRDFVAK QKEDLELAMRKLTTENRREICDKERDCLSKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRH DLLRKHEKEREQMQRYNQRMMEQLKVRQQQEKARLPKIQRSDGKTRMAMYKKSLHINGAGSASEQREKIK QFSQQEEKRQKAERLQQQQKHENQMRDMVAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQSLK EWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAEPRPTTPSKASNFFPYSSGDAS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009288 |
ORF Size | 2898 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009288.2, NP_033314.2 |
RefSeq Size | 5082 bp |
RefSeq ORF | 2901 bp |
Locus ID | 20868 |
UniProt ID | O55098 |
Gene Summary | This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222869 | Stk10 (untagged) - Mouse serine/threonine kinase 10 (Stk10), (10ug) |
CNY 12,350.00 |
|
MR220805 | Stk10 (Myc-DDK-tagged) - Mouse serine/threonine kinase 10 (Stk10) |
CNY 7,080.00 |
|
MR220805L3 | Lenti ORF clone of Stk10 (Myc-DDK-tagged) - Mouse serine/threonine kinase 10 (Stk10) |
CNY 9,030.00 |
|
MR220805L4 | Lenti ORF clone of Stk10 (mGFP-tagged) - Mouse serine/threonine kinase 10 (Stk10) |
CNY 9,030.00 |