Timm50 (BC010303) Mouse Tagged ORF Clone

CAT#: MR202741

  • TrueORF®

Timm50 (Myc-DDK-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676)

ORF Plasmid: DDK tGFP



  "BC010303" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 6,365.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2810403L02Rik; AU015082; TIM50L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202741 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCATCGAGCCCACCAGTCCCTGCCTTCTCCCAGACCCTCTGCGGGAGCCCTACTACCAGCCACCTT
ATACACTGGTCCTGGAGCTTACCGGGGTCCTTCTGCACCCAGAGTGGTCGCTGGCCACTGGCTGGAGGTT
TAAGAAGCGTCCAGGCATCGAGACCTTGTTCCAGCAGCTTGCCCCTCTGTATGAAATAGTCATCTTCACA
TCAGAGACTGGCATGACTGCATTTCCACTCATTGATAGTGTGGACCCTCACGGTTTCATCTCCTACCGTC
TGTTCCGGGACGCCACCAGATATATGGAGGGACATCATGTGAAGGACATCTCCTGTCTGAATCGGGACCC
AGCCCGAGTAGTAGTCGTGGACTGCAAGAAGGAAGCTTTCCGCCTCCAGCCCTTCAACGGTGTTGCCCTG
CGGCCCTGGGATGGCAACTCAGATGACCGGGTCTTGCTGGACCTGTCTGCCTTCCTCAAGACCATTGCAC
TGAACCAAGTGGAGGACGTCCGGACGGTGCTGGAGCACTATGCTCTAGAGGATGACCCACTGGAGGCATT
CAAACAGCGGCAGAGCCGGCTTGAGCAGGAGGAGCAGCAGCGCCTGGCCGAGCTCTCCAAGTCCAACAGG
CAGGGCCTCTCCTTTGGCTCACTCGCCAGCCGACTGTGGCCTCGCTCCAAGCAGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202741 protein sequence
Red=Cloning site Green=Tags(s)

MIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFT
SETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVAL
RPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNR
QGLSFGSLASRLWPRSKQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC010303
ORF Size 687 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010303, AAH10303
RefSeq Size 1487 bp
RefSeq ORF 689 bp
Locus ID 66525
MW 26.4 kDa
Gene Summary Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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