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Timm50 (BC010303) Mouse Tagged ORF Clone
CAT＃: MR202741
- TrueORF®
Timm50 (Myc-DDK-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676)
ORF Plasmid: tGFP
"BC010303" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810403L02Rik; AU015082; TIM50L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202741 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCATCGAGCCCACCAGTCCCTGCCTTCTCCCAGACCCTCTGCGGGAGCCCTACTACCAGCCACCTT ATACACTGGTCCTGGAGCTTACCGGGGTCCTTCTGCACCCAGAGTGGTCGCTGGCCACTGGCTGGAGGTT TAAGAAGCGTCCAGGCATCGAGACCTTGTTCCAGCAGCTTGCCCCTCTGTATGAAATAGTCATCTTCACA TCAGAGACTGGCATGACTGCATTTCCACTCATTGATAGTGTGGACCCTCACGGTTTCATCTCCTACCGTC TGTTCCGGGACGCCACCAGATATATGGAGGGACATCATGTGAAGGACATCTCCTGTCTGAATCGGGACCC AGCCCGAGTAGTAGTCGTGGACTGCAAGAAGGAAGCTTTCCGCCTCCAGCCCTTCAACGGTGTTGCCCTG CGGCCCTGGGATGGCAACTCAGATGACCGGGTCTTGCTGGACCTGTCTGCCTTCCTCAAGACCATTGCAC TGAACCAAGTGGAGGACGTCCGGACGGTGCTGGAGCACTATGCTCTAGAGGATGACCCACTGGAGGCATT CAAACAGCGGCAGAGCCGGCTTGAGCAGGAGGAGCAGCAGCGCCTGGCCGAGCTCTCCAAGTCCAACAGG CAGGGCCTCTCCTTTGGCTCACTCGCCAGCCGACTGTGGCCTCGCTCCAAGCAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202741 protein sequence
Red=Cloning site Green=Tags(s) MIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFT SETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVAL RPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNR QGLSFGSLASRLWPRSKQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010303 |
| ORF Size | 687 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010303, AAH10303 |
| RefSeq Size | 1487 bp |
| RefSeq ORF | 689 bp |
| Locus ID | 66525 |
| MW | 26.4 kDa |
| Gene Summary | Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200682 | Timm50 (untagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676), (10ug) |
CNY 2,400.00 |
|
| MG202741 | Timm50 (tGFP-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676) |
CNY 2,850.00 |
|
| MR202741L3 | Lenti ORF clone of Timm50 (Myc-DDK-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676) |
CNY 4,750.00 |
|
| MR202741L4 | Lenti ORF clone of Timm50 (mGFP-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676) |
CNY 4,750.00 |
