Ppt2 (NM_019441) Mouse Tagged ORF Clone
CAT#: MR204224
- TrueORF®
Ppt2 (Myc-DDK-tagged) - Mouse palmitoyl-protein thioesterase 2 (Ppt2)
ORF Plasmid: tGFP
"NM_019441" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 0610007M19Rik; AA672937 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204224 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGCTATGGAGGCAGAGGCTTCCTTCGGCTTGGGCTTTGCTTCTCCTGCCGTTCCTGCCGCTGC TGATGCCCGCAGCCCCCGCAGCCCACCGCGGGTCCTACAAGCCCGTGATCGTGGTGCACGGGCTCTTTGA CAGTTCATACAGCTTCCGCCACCTGCTGGACTATATCAATGAGACACACACCGGGACTGTGGTGACAGTG CTTGATCTCTTCGATGGCAGAGAGAGTTTGCGGCCCCTGTGGGAACAGGTACAAGGGTTCCGAGAGGCTG TGGTCCCCATCATGGAAAAGGCCCCTGAAGGAGTGCACCTCATCTGCTACTCCCAGGGGGGCCTGGTGTG CCGTGCTTTGCTGTCTGTCATGGATAACCACAATGTGGACTCTTTCATCTCCCTCTCCTCCCCACAGATG GGCCAGTATGGAGACACGGACTATTTGAAATGGCTCTTCCCCACGTCCATGCGGTCTAACCTCTATCGGG TCTGCTATAGTCCTTGGGGCCAGGAATTTTCCATTTGCAACTACTGGCACGATCCTCACCACGATGACTT GTACCTCAATGCCAGCAGCTTTCTGGCCCTCATCAATGGGGAAAGAGACCATCCCAATGCCACTGCATGG CGGAAGAACTTCCTTCGCGTGGGCCGTCTGGTGCTGATTGGGGGTCCTGATGATGGCGTTATCACTCCCT GGCAATCTAGCTTCTTTGGTTTCTATGATGCCAATGAGACAGTTCTGGAGATGGAGGAGCAGCCGGTGTA TCTTCGAGATTCTTTTGGGTTGAAGACTCTCCTGGCCCGGGGGGCCATAGTGAGGTGTCCCATGGCTGGC ATCTCTCACACCACGTGGCACTCTAACCGTACGCTCTACGATACTTGCATTGAGCCGTGGCTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204224 protein sequence
Red=Cloning site Green=Tags(s) MPGLWRQRLPSAWALLLLPFLPLLMPAAPAAHRGSYKPVIVVHGLFDSSYSFRHLLDYINETHTGTVVTV LDLFDGRESLRPLWEQVQGFREAVVPIMEKAPEGVHLICYSQGGLVCRALLSVMDNHNVDSFISLSSPQM GQYGDTDYLKWLFPTSMRSNLYRVCYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATAW RKNFLRVGRLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQPVYLRDSFGLKTLLARGAIVRCPMAG ISHTTWHSNRTLYDTCIEPWLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019441 |
ORF Size | 909 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019441.5 |
RefSeq Size | 1701 bp |
RefSeq ORF | 909 bp |
Locus ID | 54397 |
UniProt ID | O35448 |
MW | 34.4 kDa |
Gene Summary | Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201178 | Ppt2 (untagged) - Mouse palmitoyl-protein thioesterase 2 (Ppt2), (10ug) |
CNY 2,400.00 |
|
MG204224 | Ppt2 (tGFP-tagged) - Mouse palmitoyl-protein thioesterase 2 (Ppt2) |
CNY 2,850.00 |
|
MR204224L3 | Lenti ORF clone of Ppt2 (Myc-DDK-tagged) - Mouse palmitoyl-protein thioesterase 2 (Ppt2) |
CNY 4,750.00 |
|
MR204224L4 | Lenti ORF clone of Ppt2 (mGFP-tagged) - Mouse palmitoyl-protein thioesterase 2 (Ppt2) |
CNY 4,750.00 |