Cpsf3l (BC008240) Mouse Tagged ORF Clone

CAT#: MR204232

  • TrueORF®

Cpsf3l (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specific factor 3-like (cDNA clone MGC:11978 IMAGE:3601302)

ORF Plasmid: DDK tGFP



  "BC008240" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2410006F12Rik; AU044035
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204232 representing BC008240
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGAGTTTAAGCACATCAAAGCCTTTGACCGGACATTTGCTGACAACCCAGGTCCCATGGTTGTGT
TTGCCACACCTGGGATGCTGCATGCTGGCCAATCCCTGCAAATCTTCCGGAAGTGGGCAGGAAATGAGAA
GAACATGGTCATCATGCCTGGTTATTGTGTGCAAGGCACCGTGGGCCACAAGATCCTTAGTGGGCAACGT
AAACTGGAAATGGAAGGGCGCCAGATGCTGGAGGTAAAGATGCAAGTGGAATACATGTCCTTCAGCGCCC
ATGCTGATGCCAAGGGCATCATGCAGCTGGTAGGACAAGCGGAGCCCGAGAGTGTGCTGTTGGTGCATGG
CGAAGCCAAGAAGATGGAGTTCCTGAGGCAGAAGATTGAGCAGGAATTCCGGGTCAGCTGCTACATGCCG
GCCAATGGTGAGACAGTGACGCTGCCCACAAGCCCTAGCATCCCTGTGGGCATCTCCCTGGGATTGCTGA
AGCGGGAGATGGTGCAGGGGCTGTTGCCTGAAGCCAAAAAACCTAGGCTTTTACATGGCACCCTCATTAT
GAAAGACAGTAATTTCCGGCTGGTATCCTCAGAGCAAGCCCTGAAGGAGCTGGGCCTGGCAGAGCACCAA
CTTCGCTTCACCTGTCGTGTGCACCTGCAGGACACACGCAAAGAACAAGAAACAGCTTTACGTGTGTACA
GCCATCTCAAGAGCACCCTCAAGGACCACTGTGTACAGCATCTCCCCGATGGCTCTGTGACTGTGGAGTC
CATTCTAATCCAGGCTGCCGCCCATTCGGAGGACCCTGGTACCAAGGTGCTATTGGTCTCCTGGACTTAC
CAGGATGAAGAGCTGGGAAGCTTCCTCACAACATTGCTCAAAAATGGCCTTCCTCAGGCTCCCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204232 representing BC008240
Red=Cloning site Green=Tags(s)

MFEFKHIKAFDRTFADNPGPMVVFATPGMLHAGQSLQIFRKWAGNEKNMVIMPGYCVQGTVGHKILSGQR
KLEMEGRQMLEVKMQVEYMSFSAHADAKGIMQLVGQAEPESVLLVHGEAKKMEFLRQKIEQEFRVSCYMP
ANGETVTLPTSPSIPVGISLGLLKREMVQGLLPEAKKPRLLHGTLIMKDSNFRLVSSEQALKELGLAEHQ
LRFTCRVHLQDTRKEQETALRVYSHLKSTLKDHCVQHLPDGSVTVESILIQAAAHSEDPGTKVLLVSWTY
QDEELGSFLTTLLKNGLPQAPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC008240
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC008240.1
RefSeq Size 1222 bp
RefSeq ORF 908 bp
Locus ID 71957
MW 44.8 kDa
Gene Summary Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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