Akr1d1 (NM_145364) Mouse Tagged ORF Clone
CAT#: MR204723
- TrueORF®
Akr1d1 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member D1 (Akr1d1)
ORF Plasmid: tGFP
"NM_145364" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC25814 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204723 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCAGCGCTGCACACCACCAAATATCCCTAAGTGATGGGAACAACATTCCACTCATTGGGCTTG GAACCTACTCAGATCCTAGACCGGTCCCTGGGAAGACCTATGTGGCAGTGAAGACAGCTATTGATGAGGG GTACCGGCACATTGATGGCGCCTATGTTTACCACAATGAGCATGAAGTCGGTGAGGCCATCAGAGAAAAG ATAGCAGAAGGGAAGGTAAAGAGGGAAGAGATCTTCTACTGCGGAAAGTTATGGAATACAGAGCATGTCC CATCAATGGTCCTCCCAGCCCTGGAAAGGACGCTAAAGGCCCTCAAACTAGATTACATAGACCTTTATAT TATCGAACTGCCCATGGCCTTTAAGCCTGGAAAGGAAATTTACCCTAGAGATGAAAATGGCCGAATTATA TATGACAAAACAAATCTGTGTGCCACGTGGGAGGCATTGGAAGCTTGCAAAGATGCTGGCTTGGTGAAAT CCCTTGGGGTGTCTAATTTTAACCGCAGGCAGCTGGAGCTCATCTTGAACAAGCCAGGACTCAAGTACAA ACCAGTCACTAACCAGGTGGAGTGCCACCCGTATTTCACCCAGACAAAACTCTTGAAATTCTGCCAGCAG CATGACATCGTCATTGTCGCACATAGCCCCTTAGGGACCTGCCGCAACCCATCATGGGTGAACGTTTCTT CTCCACCCTTGTTAAATGACGAACTCCTAACCTCACTGGGAAAAAAGTACAATAAGACACAAGCTCAAAT TGTGTTGCGTTTCAACATCCAGCGAGGGATAGTTGTCATTCCTAAAAGCTTTACCCCCGAAAGGATCAAA GAAAACTTTCAGATCTTTGACTTTTCTCTCACGGAAGAAGAAATGAAGGACATTGATGCCTTGAATAAAA ATGTCCGCTATGTGGAGTTGCTCATGTGGAGTGACCATCCTGAATACCCATTTCATGACGAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204723 protein sequence
Red=Cloning site Green=Tags(s) MNLSAAHHQISLSDGNNIPLIGLGTYSDPRPVPGKTYVAVKTAIDEGYRHIDGAYVYHNEHEVGEAIREK IAEGKVKREEIFYCGKLWNTEHVPSMVLPALERTLKALKLDYIDLYIIELPMAFKPGKEIYPRDENGRII YDKTNLCATWEALEACKDAGLVKSLGVSNFNRRQLELILNKPGLKYKPVTNQVECHPYFTQTKLLKFCQQ HDIVIVAHSPLGTCRNPSWVNVSSPPLLNDELLTSLGKKYNKTQAQIVLRFNIQRGIVVIPKSFTPERIK ENFQIFDFSLTEEEMKDIDALNKNVRYVELLMWSDHPEYPFHDEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_145364 |
ORF Size | 978 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145364.1, NP_663339.1 |
RefSeq Size | 2713 bp |
RefSeq ORF | 978 bp |
Locus ID | 208665 |
UniProt ID | Q8VCX1 |
MW | 37.3 kDa |
Gene Summary | Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG204723 | Akr1d1 (tGFP-tagged) - Mouse aldo-keto reductase family 1, member D1 (Akr1d1) |
CNY 2,850.00 |
|
MR204723L3 | Lenti ORF clone of Akr1d1 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member D1 (Akr1d1) |
CNY 4,750.00 |
|
MR204723L4 | Lenti ORF clone of Akr1d1 (mGFP-tagged) - Mouse aldo-keto reductase family 1, member D1 (Akr1d1) |
CNY 4,750.00 |