Cdc5l (BC003893) Mouse Tagged ORF Clone

CAT#: MR206566

  • TrueORF®

Cdc5l (Myc-DDK-tagged) - Mouse cell division cycle 5-like (S. pombe) (cDNA clone MGC:6754 IMAGE:3593535)

ORF Plasmid: DDK tGFP



  "BC003893" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1200002I02Rik; AA408004; PCDC5RP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206566 representing BC003893
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCGGATTATGATCAAAGGGGGAGTGTGGAGGAACACGGAGGATGAAATTCTGAAAGCAGCGGTAA
TGAAATATGGGAAAAACCAGTGGTCTAGGATTGCCTCATTGCTGCATAGGAAATCGGCAAAGCAGTGCAA
AGCCAGATGGTACGAATGGCTGGATCCAAGTATTAAAAAAACTGAATGGTCCAGAGAAGAGGAAGAAAAA
CTCTTGCACCTGGCCAAGCTGATGCCAACGCAGTGGAGGACCATTGCTCCGATCATAGGAAGAACAGCAG
CCCAGTGCTTGGAACACTATGAGTTCCTCCTGGACAAAACTGCCCAAAGGGACAATGAAGAAGAAACAAC
AGATGACCCCCGAAAGCTTAAGCCTGGAGAGATAGATCCAAACCCAGAGACCAAGCCAGCCCGGCCTGAC
CCGATAGACATGGATGAGGATGAACTTGAGATGCTCTCCGAGGCTCGAGCCCGTCTGGCCAATACTCAAG
GAAAGAAGGCTAAAAGGAAAGCAAGAGAAAAGCAGTTGGAAGAAGCCAGGCGCCTTGCTGCCCTCCAGAA
AAGACGAGAGCTTCGAGCAGCTGGCATAGAGATTCAGAAGAAAAGGAAAAAGAAGAGAGGCGTTGATTAT
AATGCTGAAATCCCATTTGAAAAAAAGCCTGCTCTTGGTTTTTATGATACTTCTGAGGAGAATTACCAGG
CCCTTGATGCAGATTTCAGGAAACTGCGGCAGCAGGATCTTGATGGAGAGCTAAGATCTGAAAAAGAAGG
AAGAGATAGGAAAAAAGACAAACAGCATTTAAAAAGGAAAAAGGAGTCTGATTTACCATCAGCTATCCTC
CAAACCAGTGGTGTCTCTGAGTTCACTAAAAAGAGAAGCAAACTTGTGTTGCCTGCGCCTCAGATTTCAG
ATGCTGAACTCCAGGAAGTTGTCAAGGTAGGCCAGGCCAGTGAAGTCGCACGGCAGACGGCGGAGGAGTC
AGGAATAACAAACTCTGCTTCTAGCACTCTCCTGTCTGAGTACAATGTCACAAACAACAGCATCGCTCTG
AGAACACCACGGACACCAGCCTCTCAAGACAGGATTCTACAGGAAGCTCAGAACCTCATGGCCTTGACCA
ATGTGGACACTCCATTGAAAGGTGGGCTTAATACCCCACTGCATGAGAGTGACTTCTCTGGTGTGACCCC
ACAGCGGCAAGTTGTGCAGACTCCAAATACAGTTCTGTCTACCCCTTTCAGATTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206566 representing BC003893
Red=Cloning site Green=Tags(s)

MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEK
LLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKTAQRDNEEETTDDPRKLKPGEIDPNPETKPARPD
PIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKKKRGVDY
NAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAIL
QTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEVARQTAEESGITNSASSTLLSEYNVTNNSIAL
RTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC003893
ORF Size 1245 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC003893.1
RefSeq Size 2013 bp
RefSeq ORF 1247 bp
Locus ID 71702
MW 73.8 kDa
Gene Summary DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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