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Cdc5l (BC003893) Mouse Tagged ORF Clone
CAT＃: MR206566
- TrueORF®
Cdc5l (Myc-DDK-tagged) - Mouse cell division cycle 5-like (S. pombe) (cDNA clone MGC:6754 IMAGE:3593535)
ORF Plasmid: tGFP
"BC003893" in other vectors (3)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200002I02Rik; AA408004; PCDC5RP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206566 representing BC003893
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGGATTATGATCAAAGGGGGAGTGTGGAGGAACACGGAGGATGAAATTCTGAAAGCAGCGGTAA TGAAATATGGGAAAAACCAGTGGTCTAGGATTGCCTCATTGCTGCATAGGAAATCGGCAAAGCAGTGCAA AGCCAGATGGTACGAATGGCTGGATCCAAGTATTAAAAAAACTGAATGGTCCAGAGAAGAGGAAGAAAAA CTCTTGCACCTGGCCAAGCTGATGCCAACGCAGTGGAGGACCATTGCTCCGATCATAGGAAGAACAGCAG CCCAGTGCTTGGAACACTATGAGTTCCTCCTGGACAAAACTGCCCAAAGGGACAATGAAGAAGAAACAAC AGATGACCCCCGAAAGCTTAAGCCTGGAGAGATAGATCCAAACCCAGAGACCAAGCCAGCCCGGCCTGAC CCGATAGACATGGATGAGGATGAACTTGAGATGCTCTCCGAGGCTCGAGCCCGTCTGGCCAATACTCAAG GAAAGAAGGCTAAAAGGAAAGCAAGAGAAAAGCAGTTGGAAGAAGCCAGGCGCCTTGCTGCCCTCCAGAA AAGACGAGAGCTTCGAGCAGCTGGCATAGAGATTCAGAAGAAAAGGAAAAAGAAGAGAGGCGTTGATTAT AATGCTGAAATCCCATTTGAAAAAAAGCCTGCTCTTGGTTTTTATGATACTTCTGAGGAGAATTACCAGG CCCTTGATGCAGATTTCAGGAAACTGCGGCAGCAGGATCTTGATGGAGAGCTAAGATCTGAAAAAGAAGG AAGAGATAGGAAAAAAGACAAACAGCATTTAAAAAGGAAAAAGGAGTCTGATTTACCATCAGCTATCCTC CAAACCAGTGGTGTCTCTGAGTTCACTAAAAAGAGAAGCAAACTTGTGTTGCCTGCGCCTCAGATTTCAG ATGCTGAACTCCAGGAAGTTGTCAAGGTAGGCCAGGCCAGTGAAGTCGCACGGCAGACGGCGGAGGAGTC AGGAATAACAAACTCTGCTTCTAGCACTCTCCTGTCTGAGTACAATGTCACAAACAACAGCATCGCTCTG AGAACACCACGGACACCAGCCTCTCAAGACAGGATTCTACAGGAAGCTCAGAACCTCATGGCCTTGACCA ATGTGGACACTCCATTGAAAGGTGGGCTTAATACCCCACTGCATGAGAGTGACTTCTCTGGTGTGACCCC ACAGCGGCAAGTTGTGCAGACTCCAAATACAGTTCTGTCTACCCCTTTCAGATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206566 representing BC003893
Red=Cloning site Green=Tags(s) MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEK LLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKTAQRDNEEETTDDPRKLKPGEIDPNPETKPARPD PIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKKKRGVDY NAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAIL QTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEVARQTAEESGITNSASSTLLSEYNVTNNSIAL RTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC003893 |
| ORF Size | 1245 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003893.1 |
| RefSeq Size | 2013 bp |
| RefSeq ORF | 1247 bp |
| Locus ID | 71702 |
| MW | 73.8 kDa |
| Gene Summary | DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206566 | Cdc5l (tGFP-tagged) - Mouse cell division cycle 5-like (S. pombe) (cDNA clone MGC:6754 IMAGE:3593535) |
CNY 7,088.00 |
|
| MR206566L3 | Lenti ORF clone of Cdc5l (Myc-DDK-tagged) - Mouse cell division cycle 5-like (S. pombe) (cDNA clone MGC:6754 IMAGE:3593535) |
CNY 4,280.00 |
|
| MR206566L4 | Lenti ORF clone of Cdc5l (mGFP-tagged) - Mouse cell division cycle 5-like (S. pombe) (cDNA clone MGC:6754 IMAGE:3593535) |
CNY 4,280.00 |
