Sytl4 (BC026819) Mouse Tagged ORF Clone

CAT#: MR208061

  • TrueORF®

Sytl4 (Myc-DDK-tagged) - Mouse synaptotagmin-like 4 (cDNA clone MGC:25236 IMAGE:4488012)

ORF Plasmid: DDK tGFP



  "BC026819" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,736.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Gph, Slp4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208061 representing BC026819
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGAGATACTAGACCTCTCTTTTCTGTCCGAGATGGAAAGGGATTTGATCCTCGGTGTTCTGCAGA
GAGATGAAGAACTCAGAAAAGCAGATGAGAAAAGGATTCGGCGACTGAAGAATGAGCTCCTGGAGATCAA
AAGAAAAGGGGCCAAAAGGGGCAGCCAACACTATAGTGATCGAACCTGTGCCAGGTGCCAGGAGGGCCTG
GGCCGCTTAATTCCCAAAAGCAGCACTTGTGTGGGCTGCAATCATTTAGTGTGCCGAGAATGCCGAGTTC
TGGAAAGCAATGGCTCTTGGAGGTGCAAGGTGTGCTCGAAGGAGATAGAGCTGAAGAAAGCAACTGGAGA
TTGGTTTTATGACCAGAAAGTAAATCGCTTTGATTACCGAACAGGCAGCGAGATAATCAGGATGTCCCTG
CGCCAGAAGCCTGCAGTGAATAAAAGAGAGACAGCGGGGCAGTCCCTCCTTCAGCAGACACAGATGGGTG
ATATCTGGCCAGGAAGAAGGATCATTCAAGAACAACAACAGAGGGAACAAAGTGTGCTGTTTGAAGTGCC
AAAGACCAGAAGTGGAAAGAGTGCACTGGAGGCTGAGAGCGAGAGTCTGGATAGCTACACTGCTGACTCA
GACAGCACCTCCAGGAGAGATTCTCTGGATAAATCTGGACTCTTCCCAGAATGGAAGAAGATGTCCGCTC
CCAAATCTCAAGTTGAAAAGGAAATTCCACCAGGGAACCAAAATGCAGTCTGTGGTGATGAGGGTGACAT
GGTATTCAAGAAGAACACCAAAAAAGTCCTGAGGCCTTCAGAATACACTAAGTCTGTGATAGACCTTCGC
CCAGAAGATGTGGCCCAAGAAAGTGGCATCTTGGGAGACAGAAGCAAATCTGTACCAGGCCTCAGTGTGG
ATATGGAAGAAGAGGAGGAGGAGGAAGAGGACATTGACCACCTGGTGAAGTTGCACCGCCAGAAACTGGC
CAGAGGCAGCATGCAGAGTGGCTCCTCCATGAGTACCCTTGGCAGCATTATGAGTATCTATAGCGAAGCT
GGTGATTTTGGGAACATCTCTGTGACGGGCAAGATTGCCTTTTCACTGAAGTTTGAGCAGAAAACACAGA
CTTTGGTCATCCATGTCAAGGAGTGCCACCAGCTGGCCTATGCTGATGAAGCCAAGAAGCGTTCTAACCC
ATATGTGAAGACTTATCTTCTGCCTGACAAGTCCCGCCAAGGAAAAAGAAAAACCAGCATCAAGCGGGAC
ACCATCAATCCACTATATGATGAGACCTTTCGGTACGAGATTTCGGAATCTCTTCTGGCTCAGAGGACTT
TGCAGTTTTCCGTTTGGCATCATGGTCGTTTTGGCAGAAACACTTTCCTTGGAGAGGCGGAGGTCCACAT
GGACTCTTGGAAGCTGGATAAGAAGCTGGATCACTGCCTCCCTTTACATGGAAAGGTAGTCTGCTTTAAA
GGTCGTTCACAGCTTGCTTCCCAGGTGAATTGTCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208061 representing BC026819
Red=Cloning site Green=Tags(s)

MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGL
GRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSL
RQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADS
DSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLR
PEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEA
GDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRD
TINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKVVCFK
GRSQLASQVNCL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC026819
ORF Size 1506 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC026819.1
RefSeq Size 2997 bp
RefSeq ORF 1508 bp
Locus ID 27359
MW 109.9 kDa
Gene Summary Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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