Map6 (NM_001048167) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2810411E12Rik; Map-6; Mtap6; STOP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR208950 representing NM_001048167, codon optimized. Due to the complexity of NM_001048167, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGGCCATGCATCACTCGGGCCTGCTGCATTGCCCGGTTTTGGAACCAGTTGGACAAGGCAGACA TTGCGGTTCCACTCGTGTTCACTAAGTATAGCGAGGCGACTGAGCACCCCGGCGCTCCACCTCAGCCACC TGCCCCACTGCAGCCTGCTCTCGCCCCTCCTTCCCGGGCTGTGGCGATTGAAACACAACCCGCGCAAGGA GAGTCCGACGCCGTCGCTCGGGCGACAGGACCAGCCCCAGGACCATCCGTGGACCGCGAGACGGTGGCCG CACCAGGACGGTCTGGGCTGGGGCTGGGAGCCGCTAGCGCCTCTACCAGCGGCAGTGGCCCGGCAGACTC CGTTATGAGACAAGACTACAGAGCTTGGAAGGTACAGAGGCCCGAACCTAGCTGCAGACCACGCTCAGAG TATCAGCCCAGTGACGCCCCTTTCGAGCGAGAAACTCAGTATCAGAAGGATTTCCGGGCCTGGCCCCTGC CGAGGAGGGGGGATCACCCTTGGATCCCCAAGCCAGTGCAGATCCCAGCAACATCACAGCCTTCTCAGCC CGTACTGGGAGTGCCGAAACGCAGACCTCAGTCACAGGAACGAGGTCCCATGCAGCTCAGCGCTGATGCC CGCGATCCTGAAGGGGCCGGGGGGGCAGGTGTCCTTGCCGCTGGCAAAGCTTCAGGAGTGGATCAGAGAG ATACGAGACGGAAAGCCGGACCCGCCTGGATGGTCACACGCAATGAAGGACACGAGGAGAAACCGCTGCC ACCGGCTCAATCCCAAACCCAGGAGGGAGGGCCTGCTGCTGGGAAGGCCTCAGGCGCTGATCAGAGAGAT ACCCGGAGGAAGGCCGGCCCAGCGTGGATGGTGACACGGAGCGAAGGTCATGAGGAGAAGCCACTCCCCC CAGCACAGTCTCAAACCCAGGAGGGTGGACCTGCAGCCGGTAAAGCATCCGGTGCCGACCAGCGAGACAC CCGCCGGAAAGCCGGGCCAGCATGGATGGTGACTAGGACTGAAGGCCATGAGGAGACACCTCTCCCGCCA GCCCAATCTCAGACACAGGAGGGGGGGCCTGCCGCTGGAAAGGCGTCCGGAGCTGACGAGCGGGATACAA GACGGAAGGCAGGACCGGCGTGGATGGTTCGGCGGAGCGAGGGCCATGAGCAAACCCCTGCTGCACATGC ACAGGGCACCGGGCCTGAAGGGGGTAAAGGGCGGGCTGTGGCCGACGCCCTGAACCGCCAGATCCGCGAG GAGGTGGCATCTACAGTCTCATCAAGTTATCGCAACGAATTCCGGGCTTGGACCGACATCAAGCCAGTGA AACCTATTAAAGCTAAGCCACAATATAAGCCTCCTGACGATAAGATGGTGCACGAGACAAGTTACAGCGC TCAGTTCAAAGGAGAAGCAAATAAGCCTAGCGCCGCTGATAATAAAGCCATGGATAGACGGAGAATTCGG TCCCTCTATAGTGAGCCCTTCAAGGAGTGTCCCAAGGTTGAGAAGCCCAGCGTCCAGAGTAGTAAGCCTA AGAAAACGAGTACCTCCCATAAGCCCCCAAGGAAGGCTAAAGACAAGCAGGTGGTTTCCGGGCAAGCCGC GAAGAAAAAGACCACAGAGGGACCTTCCGCCACGAAACCTGATGATAAGGAGCAGTCCAAAGAAATGAAC AATAAGCTCGCCGAGGCCAAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR208950 representing NM_001048167 Red=Cloning site Green=Tags(s) MAWPCITRACCIARFWNQLDKADIAVPLVFTKYSEATEHPGAPPQPPAPLQPALAPPSRAVAIETQPAQG ESDAVARATGPAPGPSVDRETVAAPGRSGLGLGAASASTSGSGPADSVMRQDYRAWKVQRPEPSCRPRSE YQPSDAPFERETQYQKDFRAWPLPRRGDHPWIPKPVQIPATSQPSQPVLGVPKRRPQSQERGPMQLSADA RDPEGAGGAGVLAAGKASGVDQRDTRRKAGPAWMVTRNEGHEEKPLPPAQSQTQEGGPAAGKASGADQRD TRRKAGPAWMVTRSEGHEEKPLPPAQSQTQEGGPAAGKASGADQRDTRRKAGPAWMVTRTEGHEETPLPP AQSQTQEGGPAAGKASGADERDTRRKAGPAWMVRRSEGHEQTPAAHAQGTGPEGGKGRAVADALNRQIRE EVASTVSSSYRNEFRAWTDIKPVKPIKAKPQYKPPDDKMVHETSYSAQFKGEANKPSAADNKAMDRRRIR SLYSEPFKECPKVEKPSVQSSKPKKTSTSHKPPRKAKDKQVVSGQAAKKKTTEGPSATKPDDKEQSKEMN NKLAEAKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001048167
ORF Size	1704 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001048167.1, NP_001041632.1
RefSeq Size	4962 bp
RefSeq ORF	1707 bp
Locus ID	17760
UniProt ID	Q7TSJ2
MW	61.1 kDa
Gene Summary	Involved in microtubule stabilization in many cell types, including neuronal cells (PubMed:9600916). Specifically has microtubule cold stabilizing activity (PubMed:9600916). Involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking via its interaction with TMEM106B (By similarity). Regulates KIF5A-mediated axonal cargo transport (By similarity). Regulates axonal growth during neuron polarization (PubMed:28521134).[UniProtKB/Swiss-Prot Function]

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