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D8Ertd354e (BC024084) Mouse Tagged ORF Clone
CAT＃: MR209530
- TrueORF®
D8Ertd354e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 354, expressed (cDNA clone MGC:37875 IMAGE:5101064)
ORF Plasmid: tGFP
"BC024084" in other vectors (4)
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CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9430010M12Rik; AW208455; D8Ertd354e; Tmem76 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209530 representing BC024084
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCGGGCCCGGCGCTGGCAGCGCTGCTGCTGGCCGGGTCGGTGCTGAGCGCCACGCTGCTGGCCC CCGGCCGCCGGGCGGAGCCAGATTTAGATGAAAAAAGGAACGTGGAGCTGAAGATGGATCAGGCTTTGCT GCTTATTCACAATGAACTCTTGGGGACGAGCCTGACTGTGTACTGGAAATCTGACGACTGTTACCAATGC ACCTTCCAGCCACTGGCGAACGTCTCTCATGGTGGAAAGCCAGCAAAGCCAAGCGTGGCACCTGTCTCTG TGAGCACCCAGCACGGGTCCATCCTGCAGGTTAACTCCACCTCGGAGGAGAGAGCAGCTTGCAGGCTGGA ATACAAATTTGGAGAATTTGGAAACTATTCCCTTTTGGTGCAGCATGCTTCCAGCGGGGCCAATAAAATT GCCTGTGACATCATCGTCAATGAGAATCCAGTGGACAGTAACCTTCCTGTGAGCATCGCATTCCTTGTTG GTCTAGCCCTCATCGTCGCAGTATCCCTCCTGAGGCTCTTGTTGAGTTTGGATGATGTCAATAACTGGAT ATCTAAGGCAATAGCTTCTCGAGAAACTGACCGTCTCATCAATTCTGAGCTAGGGTCTCCCAGCAGAGCA GACCCTCTCAGCGCTGATTACCAACCAGAAACTCGGCGTTCTTCTGCGAACCGTCTCCGATGTGTGGACA CATTTAGGGGGTTAGCTCTCGTCCTCATGGTCTTTGTTAACTATGGAGGAGGAAAGTATTGGTACTTCAA ACATTCAAGTTGGAACGGACTGACTGTGGCCGACCTCGTCTTCCCATGGTTTGTATTTATTATGGGAACT TCAATTTTCCTATCAATGACTTCTATCCTGCAACGAGGATGTTCAAAACTCAAACTGCTGGGGAAGATTG TGTGGAGAAGCTTCCTGCTAATCTGTATAGGAGTTATCATTGTCAACCCCAATTATTGCCTTGGTCCATT GTCTTGGGATAAAGTGCGGATCCCTGGTGTCCTGCAGCGGTTGGGAGTGACATACTTCGTGGTCGCTGTG TTGGAGTTCTTTTTCTGGAAGCCTGTGCCTGACAGTTGTACCTTGGAAAGCAGCTGCTTCTCTCTCCGAG ACATCACGTCCAGCTGGCCCCAGTGGCTCACCATTCTGACGCTGGAAAGCATTTGGCTGGCTTTGACATT CTTTCTGCCTGTCCCTGGGTGCCCTACTGGCTACCTTGGCCCTGGCGGTATAGGGGACTTGGGGAAGTAT CCTCATTGTACTGGAGGAGCAGCTGGCTACATCGACCGCCTGCTCCTGGGAGACAATCACCTGTACCAGC ACCCATCCTCCACCGTGCTGTATCACACAGAGGTGGCCTACGACCCTGAAGGAGTCTTAGGGACTATCAA CTCAATTGTGATGGCGTTTTTAGGAGTTCAGGCAGGAAAAATACTGGTGTATTACAAGGATCAGACGAAA GCCATCCTGACCCGATTTGCCGCCTGGTGCTGTATCCTTGGGCTTATTTCTATTGTGTTGACAAAAGTGT CTGCAAATGAAGGTTTTATTCCAATAAACAAGAATCTCTGGTCCATCTCGTACGTCACCACACTGAGCTG CTTTGCCTTCTTCATCCTCCTCATTCTCTACCCAGTTGTGGATGTGAAGGGCCTGTGGACGGGAACCCCC TTCTTCTATCCAGGGATGAACTCCATCCTGGTGTATGTTGGCCACGAGGTGTTGGAGAACTACTTCCCCT TCCAGTGGAAGCTGGCGGATGAGCAGTCACACAAAGAGCATCTCATTCAGAACATAGTCGCCACGGCTCT CTGGGTGCTCATCGCCTACGTGCTCTATAAAAAGAAGCTGTTTTGGAAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209530 representing BC024084
Red=Cloning site Green=Tags(s) MGAGPALAALLLAGSVLSATLLAPGRRAEPDLDEKRNVELKMDQALLLIHNELLGTSLTVYWKSDDCYQC TFQPLANVSHGGKPAKPSVAPVSVSTQHGSILQVNSTSEERAACRLEYKFGEFGNYSLLVQHASSGANKI ACDIIVNENPVDSNLPVSIAFLVGLALIVAVSLLRLLLSLDDVNNWISKAIASRETDRLINSELGSPSRA DPLSADYQPETRRSSANRLRCVDTFRGLALVLMVFVNYGGGKYWYFKHSSWNGLTVADLVFPWFVFIMGT SIFLSMTSILQRGCSKLKLLGKIVWRSFLLICIGVIIVNPNYCLGPLSWDKVRIPGVLQRLGVTYFVVAV LEFFFWKPVPDSCTLESSCFSLRDITSSWPQWLTILTLESIWLALTFFLPVPGCPTGYLGPGGIGDLGKY PHCTGGAAGYIDRLLLGDNHLYQHPSSTVLYHTEVAYDPEGVLGTINSIVMAFLGVQAGKILVYYKDQTK AILTRFAAWCCILGLISIVLTKVSANEGFIPINKNLWSISYVTTLSCFAFFILLILYPVVDVKGLWTGTP FFYPGMNSILVYVGHEVLENYFPFQWKLADEQSHKEHLIQNIVATALWVLIAYVLYKKKLFWKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC024084 |
| ORF Size | 1872 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC024084.1 |
| RefSeq Size | 2417 bp |
| RefSeq ORF | 1874 bp |
| Locus ID | 52120 |
| MW | 88.6 kDa |
| Gene Summary | Lysosomal acetyltransferase that acetylates the non-reducing terminal alpha-glucosamine residue of intralysosomal heparin or heparan sulfate, converting it into a substrate for luminal alpha-N-acetyl glucosaminidase.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218305 | D8Ertd354e (untagged) - Mouse DNA segment, Chr 8, ERATO Doi 354, expressed (cDNA clone MGC:37875 IMAGE:5101064), (10ug) |
CNY 6,940.00 |
|
| MG209530 | D8Ertd354e (tGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 354, expressed (cDNA clone MGC:37875 IMAGE:5101064) |
CNY 6,940.00 |
|
| MR209530L3 | Lenti ORF clone of D8Ertd354e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 354, expressed (cDNA clone MGC:37875 IMAGE:5101064) |
CNY 8,170.00 |
|
| MR209530L4 | Lenti ORF clone of D8Ertd354e (mGFP-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 354, expressed (cDNA clone MGC:37875 IMAGE:5101064) |
CNY 8,170.00 |
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